Document Type: Original Article


1 MSc. Student, Department of biotechnology, Faculty of agriculture, Buali Sina University,Hamedan, Iran

2 Department of biotechnology, Faculty of agriculture, Buali Sina University, Hamedan, Iran


Objective: In order to use natural compounds in controlling plant pests and diseases, many researchers in recent years have studied the antifungal effects of essential oils and plant extracts.  The purpose of this study was to investigate the chemical composition and antifungal effects of Echium khuzestanium and marubbium anisodan extracts. Methods: After extraction and preparation of different concentrarion from extracts, antifungal effect on 4 plant fungi was stadied by disc diffusion method. chemical composition of plants was checked by GC/MS. Results: According to the results, 86 kinds of chemical compounds found in M.anisodan extract. Furfural, steroids, vitamin B and flavonoids are the main compounds of M.anisodan. 46 kinds of chemical compounds found in methanol extract of E.khuzestanium. there are mucilage, fatty acids, flavonoid and diterpenes in flower of E.khuzestanium.


Main Subjects

Alam, M.H., Murat, E., Siegfrie, N. and Hubert, K. (2007). Chemical composition and content of essential oil from the bud cultivated Turkish clove (Syzygium aromaticum L. Bio Resources, 2 (2): 265-269.
Azlan, G. J., Madzali, M and Johari, R. (2003). Accumulation of physalin in cell and tissues of physalis minimal. L. III WOCAMP Congress on Medicinal and
Aromatic Plant.
Cowan, M.M. (1999). Plant products as antimicrobial agents. Clinical Microbiology Reviews, 12(4):564-582.
Delorme, P., Jay, M. and Ferry, S. (1977). Iventaire phytochimique des borraginacees indigenes. Planta Med, 11:5ñ11.
Dixon, R. A. and Steele, C. L. (1999). Flavonoids and isoflavonoids - a gold mine for metabolic engineering. Trends in Plant Science, 4: 394-400.
Ebrahimzadeh, M.A., Pourmorad, F. and Bekhradnia, A.Z. (2008). Iron chelating activity, phenol and flavonoid content of some medicinal plants from Iran. African Journal of Biotechnology, 7 (18):3188-3192.
Ghorbani, A.B. (2004). Ethnobotanical Survey in Golestan Province and Adjacent Regions. (M.Sc. Thesis), Faculty of Science, Tehran University, Tehran.
Horrobin, DF. (1992). Nutritional and medical importance of gamma-linolenic acid. Progress in lipid research, 31: 163-194.
Javadzadeh, S.M. (1995). The effect of sowing methods on, nitrogen fertilizer and plant density on quality and quantity yield of Borago officinalis. L.M.S thesis Islamic Azad University of Jiroft, Iran.
Khatamsaz, M. (2002). Flora of Iran, no. 39: Boraginaceae. Research Institute of forests and rangelands, Tehran.
Meyre-silva, c. and Cechinel-Filho, V. (2010). a review of the chemical and pharmacological aspects of the genus Marrubium. Curr pharm des, 16(31): 3503- 18.
Muyima, N. Y. O., Nziweni, S. and Mabinya, L. V. (2004). Antimicrobial and antioxidant activities of Tagetes mimuta, Lippia javanica, and Foeniculum vulgare essential oils from eastern cape province of south Africa. JEOBP, 7: 68-78.
Narayanasamy, P.N. (2002). Microbial Plant Pathogens and Crop Disease Management. Science Publishers USA. Osborne, J.L. (1999). borage. Bee world, 80: 33. 

Papageorgiou, V.P., Assimopoulou, A.N. and Kyriacou, G.
(2002). Determination of naturally occurring hydroxynaphthoquinone polymers by size-exclusion chromatography. Chromatographia, 55:423ñ430.
Papageorgiou, V.P., Assimopoulou, A.N., Samanidou, V.F. and Papadoyannis, I.N. (2006b). Recent advances in chemistry, biology and biotechnology of alkannins and shikonins. Curr. Org. Chem, 10:2123ñ2142.
Papageorgiou, V.P., Assimopoulou, A.N., Samanidou, V.F. and Papadoyannis, I.N. (2006a). Analytical methods for the determination of alkannins and shikonins. Curr. Org. Chem, 10:583ñ622.
Papageorgiou, VP., Assimopoulou, AN., Couladouros, EA., Hepworth, D. and Nicolaou, K.C. (1999). The chemistry and biology of alkannin, shikonin and related naphthazarin natural products. Angew Chem. Int. Ed, 38:270ñ301.
Pitaroki, D., Tzakou,O., Loukis, A. and Harvala, C. (2003). Volatile metabolites from Salvia fruticosa as antifungal agents in soil-borne pathges. Jorma. Agri. Food Chem, 51: 3249-3301.
Rodriguez, D. J., Castillo, D. H., Garcia, R. R. and Sanchez, J. L. A. (2005). Antifungal activity of Aloe Vera pulp and liquid fraction against plant pathogenic fungi. Industrial Crops and Products, 21:81-87.
Sahpaz, S., Garbacki, N., Tits, M and Bailleul, F. (2002). Isolation and pharmacological activity of phenylpropanoid esters from Marrubium vulgare. J.
Ethnopharmacol. 79(3): 389-392.
Shimoni, M., Puteviesk,E., Ravid, V. and Revin, R. (1993). Antifungal activity of volatile fraction of essential oils from four aromatic wild plants in Israel. J. Chem. Ecol, 19: 1129-1133.
Weel, K.C.G., Venskutonis, P.R., Pukalskas, A., Gruzdiene, D., Linssen, J.P.H. (1999). Antioxidant activity of horehound (Marrubium vulgare) grown in Lithuania. Fett/Lipid, 101(10): 395-400.
Zied, Z., Adel, K., Ines, B.C., Riadh, B.M., Ahmed, B., Hafedh, M and NÈji, G. (2011). The in-vitro evaluation of antibacterial, antifungal and cytotoxic properties of Marrubium vulgare L. essential oil grown in Tunisia.
Lipids in Health and Disease, 10:161.