Document Type: Short communication

Authors

1 Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, Iran

2 Young Researcher Society. Department of Biotechnology, Faculty of Agricultural. Shahid Bahonar University of Kerman, Kerman, Iran

3 Zabol university, Zabol, Iran

4 Institute of Agricultural Biotechnology, University of Zabol, Zabol, Iran

Abstract

Objective: Development of resistance against many of the commonly used antibiotics is an impetus for further efforts to search for new antimicrobial agents. The aim of the study was determined as antibacterial activity of silver nanoparticles produced by Plantago ovata seed extract against Pseudomonas aeruginosa. Methods: All 30 strains of P. aeruginosa wereisolated from isolates of the urinary tract infection of Hospital and the minimum inhibitory concentrations were distinguished by microdulition method. Results: The silver nanoparticles revealed Gaussian distributions with average diameter of 13 nm with some deviations.The result of plant extraction showed that the most MIC value was 100 ppm concentration, and 9 strains of pseudomonas were inhibited. Conclusion: Ag nanoparticles prepared by the effective cost reduction method described here which is greatly promising as antimicrobial agents. Applications of Ag nanoparticles based on these findings may lead to valuable discoveries in various fields such as medical devices and antimicrobial systems.

Keywords

Main Subjects

Diekema DJ, Pfaller MA, Jones RN et al. 1997.Survey of bloodstream infections due to gram-negative bacilli: frequency of occurrence and antimicrobial susceptibility of isolates collected in the United States. Canada, and Latin America for the SENTRY Antimicrobial Surveillance Program. Clin Infect Dis 1999; 29: 595ñ607.
Bernardini J, Piraino B, Sorkin M. 1987.Analysis of continuous ambulatory peritoneal dialysis-related Pseudomonas aeruginosa infections. Am J Med. 83: 829ñ 832.
Shahverdi A R, Fakhimi A, Shahverdi HR and Minaian S. 2007. Synthesis and effect of silver nanoparticles on the antibacterial activity of different antibiotics against Staphylococcus aureus and Escherichia coli. NanomedNanotechnol.3: 168-171.
Silva Paula M M d, Franco CV, Cesar BM, Rodrigues L, Barichello T, Savi G D, Bellato L.F, Fiori MA, and Silva Ld. 2009. Synthesis, characterization and antibacterial activity studies of poly-{styrene-acrylic acid} with silver
nanoparticles. Mater. Sci. Eng. 29: 647-650.

Baron EJ, Sydney M. Diagnostic Microbiology fine gold.1990. 8th Ed. 398-399.
Kim JS, Kuk E, et al.2007. Antimicrobial effects of silver nanoparticles. Nanomedicine .3: 95-101.
Soo-Hwan K, Lee HS, Ryu DS, Choi SJ, and Lee DS. 2011. Antibacterial Activity of Silver-nanoparticles Against Staphylococcus aureus and Escherichia coli. Korean J. Microbiol. Biotechnol. 39(1): 77ñ85.
Shameli K, Ahmad MB, Jazayeri SD, Shabanzadeh P, Sangpour P, Jahangirian H and Gharayebi Y. 2012. Investigation of antibacterial properties silver
nanoparticles prepared via green method Chemistry Central Journal. 6:73
Maribel G. Guzmϝn, Jean Dille, Stephan Godet. 2009. Synthesis of silver nanoparticles by chemical reduction method and their antibacterial activity. International Journal of Chemical and Biomolecular Engineering 2:3
Feng QL, Wu J, Chen GQ, Cui FZ, Kim TM, Kim JO, BiomedJ.2000. Mater. Res. 52: 662.
Spadaro JA, Berger TJ, Barranco SD, Chapin SE, Becker RO. 1974. Microb. Agents Chemother. 6 : 637.