Document Type : Original Article
Department of Biological sciences and Biotechnology, Faculty of science, University of Kurdistan, Sanandaj, Iran
Objective: Alpha-glucosidase, as a carbohydratase catalyses the liberation of alpha-glucose from the nonreducing end of some carbohydrates from foods in digestive tract. Consequently, retard absorption of glucose by the inhibition of carbohydrate hydrolyzing enzymes is one of the therapeutic approaches to decrease the postprandial hyperglycemia. Recently, a great deal of attention has been paid to natural products like plants, as a reliable source for bioactive compounds.The aim of this study was to find new potent inhibitors for Alpha-glucosidase among plant extracts. Methods: Hexan extracts of 60 plants species were screened for their Alpha-glucosidase inhibitory activity at 0.001, 0.01, 0.1 and 1 mg/mL concentration. Acarbose, dissolved in buffer, was used as a positive control. Every experiment was done in triplicate.Descurania Sophia (L.) Webb & Berth, Fumaria vailantii Loisel, Ferula Haussknechti Wolf ex Rech,Haplophyllum acutifolium (DC.)G.Don, Isatis cappadociaca Desv., Eremostachys laevigata Bunge, Silene aucheriana Boiss exhibited more than 60% alpha-glucosidase inhibitory activity.. Results: Among them, Descurania Sophia, Fumaria vailantii and Ferula haussknechti exhibited significant alpha glucosidase inhibitory activity with IC50 values of 0.9 µg/ml, 24.55 µg/ml and 2.71 µg/ml, respectively. Because of its high inhibitory activity and low IC50, Descurania Sophia extract would be interesting for further studies
Ankita BJ, Mohamed SS and Kavitha T. (2011). Screening of fifteen indian ayurvedic plants for alpha-glucosidase inhibitory activity and enzyme kinetics. International Journal of Pharmacy and Pharmaceutical Science. 3:267-
Bougneres P, Valleron AJ. (2008) Causes of early-onset type 1 diabetes: toward data-driven environmental appro alpha-glucosidase s, J. Exp. Med. 205: 2953-2957.
Brindis F, Andrade MG, Trujano MEG, Soto ES, and Molina RV. (2014). Postprandial glycaemia and inhibition of α- glucosidase activity by aqueous extract from Coriandrum sativum. Natural product research, 28(22):2021-2025.
Chen HM,Yan XJ, Lin W. (2005) Progress in-glycosidase inhibitors. Mar. Sci. 29: 73–76.
Cooke DW, Plotnick L. (2008) Type 1 diabetes mellitus in pediatrics. Pediatr Rev. 29(11): 374-384. Domains,” The Journal of Biological Chemistry, 285 (23):17763-17770.
Eidi M, Eidi A, Saeidi A, Molanaei S, Sadeghipour A, Bahar M, and Bahar K. (2009). Effect of coriander seed (Coriandrum sativum L.) ethanol extract on insulin release from pancreatic beta cells in streptozotocin‐induced diabetic rats. Phytotherapy Research, 23(3): 404-406.
Gurudeeban S, Satyavani K, and Ramanathan T. (2012) Alpha glucosidase inhibitory effect and enzyme kinetics of coastal medicinal plants. Bangladesh Journal of Pharmacology 7: 186-191.
Heiner L. (2002) Acarbose- an update of its therapeutic use in diabetes treatment, Clin Drug Invest. 22: 141-156.
Ieyama T, Gunawan-Putteri MDPT, and Kawabata J. (2011). α-Glucosidase inhibitors from the bulb of Eleutherine Americana. Food chemistry, 128: 308-311.
Kumar S, Kumar V, and Prakash O. (2013). Enzymes inhibition and antidiabetic effect of isolated constituents from Dillenia indica. BioMed research international, Article ID 382063,7 pages.
Lawag IL, Aguinaldo AM, Naheed S, and Mosihuzzaman M. (2012). α-Glucosidase inhibitory activity of selected Philippine plants. Journal of Ethnopharmacology. 144, 217-219.
Lee YJ, Kim NS, Kim H, Yi JM, Oh SM, Bang OS , Lee J. (2013) Cytotoxic and anti-inflammatory constituents from the seeds of Descurainia sophia. Archives of pharmacal research. 36(5): 536-541.
Li J, Liu X, Dong F, Xu J, Zheng Y, Shan W. (2010) Determination of the volatile composition in essential oil of Descurainia sophia (L.) Webb ex Prantl (Flixweed) by gas chromatography/mass spectrometry (GC/MS). Molecules, 15(1): 233-240.
Li YQ, Zhou FC, Gao F, Bian JS and Shan F. (2009) Comparative evaluation of quercetin, isoquercetin and rutin as inhibitors of alpha‑glucosidase. J Agric Food Chem 57: 11463-11468.
Loizzo MR, Saab AM, Tundis R, Menichini F, Bonesi M, Piccolo V.(2008). In vitro inhibitory activities of plants used in Lebanon traditional medicine against angiotensin converting enzyme (ACE) and digestive enzymes related
to diabetes. Journal of ethnopharmacology, 119(1): 109-116.
Mary G, Ryan AR, Kenneth AR, Freedland RE, Patrick JL. (2001) Association of derepression and diabetes complication: ameba-analysis psychosis, Psychosom. Med. 63: 619-630.
Matsui T. (1996). Invitro survey of alpha-glucosidase inhibitory food components. Biosci. Biotech. Biochem, 60(12), 2019-2022
Pistia-Brueggeman G, Hollingsworth RI. (2003) The use of the o-nitrophenyl group as a protecting/activating group for 2-acetamido-2-deoxyglucose. Carbohydrate Research. 338:455-458.
Puls W, Keup U, Krause HP, Thomas G, Hoffmeister F. (1977) Glucosidase inhibition. A new approach to the treatment of diabetes, obesity, and
hyperlipoproteinaemia. Naturwissenschaften 64: 536-537.
Puls W, Keup U. (1973) Influence of an -amylase inhibitor (BAY d 7791) on blood glucose, serum insulin and NEFA in starch loading tests in rats, dogs and man. Diabetologia 9(2): 97101.
Seo EJ, Curtis‑Long MJ, Lee BW, Kim HY, Ryu YB, Jeong TS, Lee WS and Park KH. (2007) Xanthones from Cudrania tricuspidata displaying potent
alpha‑glucosidase inhibition. Bioorg Med Chem Lett 17:6421-6424.
Shai LJ, Masoko P, Mokgotho MP, Magano SR, Mogale AM, Boaduo N, and Eloff JN.(2010). Yeast alpha glucosidase inhibitory and antioxidant activities of six medicinal plants collected in Phalaborwa, South Africa. South African Journal of Botany, 76(3): 465-470.
Shim Y J, Doo HK, Ahn SY, Kim YS, Seong JK, Park IS, and Min BH.(2003). Inhibitory effect of aqueous extract from the gall of Rhus chinensis on alpha-glucosidase activity and postprandial blood glucose. Journal of
ethnopharmacology, 85(2): 283-287.
Sim L, Willesma C, Mohan S, Naim YH, Pinto BM and Rose DR. (2010) Structural Basis for Substrate Selectivity in Human Maltase-Glucoamylase and Sucrase-Isomaltase NTerminal
Stout RW. (1990) Insulin and atheroma. 20-yr perspective. Diabetes Care 13: 631–654.
Vural C. (2009) A new combination in Descurainia (Brassicaceae) from Turkey. Annales Botanici Fennici. 46(1): 65-66.