Document Type: Original Article


1 Department of Veterinary Medicine, University of Urmia, Urmia, Iran

2 Assistant Professor, Department of Anatomy and Embryology, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran



Spermatogenesis process during which the male germ cells are produced at each stage of the disorder, it can lead to infertility. The aim of this study was to evaluate the effect of fennel and yarrow on spermatogenesis in adult micereceiving chemotherapy is cyclosporine. For this study, 32 male Wistar rats with an average weight of 20 ± 200 g were randomly divided into four groups (control, cyclosporine A, cyclosporine and aqueous extracts of yarrow, cyclosporine and aqueous extract of fennel). Three groups of mice at a dose cyclosporine day / kg / mg40 dissolved in half ml sesame oil, received daily. The other two groups, water extracts of yarrow and fennel with a dose of day / kg / mg 150 received. After 21 and 45 days, all rats were euthanized, the testis and epididymis were separated in the next stage of sperm characteristics, morphological changes and morphological index were Trzya by spermatogenesis. Administration of cyclosporine A significant reduction in the number and motility of sperm, as well as a significant increase in the amount of dead and abnormal sperm has caused. If the plant extracts of yarrow and fennel is to improve these parameters. The results of this study show that cyclosporine A by oxidative stress can damage the testes provide, while the aqueous extract of yarrow and fennel with scavenging free radicals and oxidative processes can be reduced the adverse effects of this drug.


Main Subjects

Abed, M.J., Bermejo, P., Gonzales, E., 1999. Antiviral activity of Bolivian plant extracts. Gen. pharmacol., 32, 499-503.
Agarwal, A., Nallella, K.P., Allamaneni, S.S., Said, T.M., 2004. Role of antioxidants in treatment of male infertility: anoverview of the literature. Reprod. Biomed. Online., 8, 616-627.
Ahero. Irancoloredflora. Forests and Rangelands Research Institute, 1365. 8(886).
Aitken, R.J., West, K.M., 1990. Analysis of the relationship between reactive oxygen species production and leucocyte in filtration in fractions of human semen separated on percoll gradients. Int. J. Androl., 13, 433-451.
Benedek, B., Kopp, B., Melzig, M.F., 2007. Achilleamillefolium L. is the anti-inflammatoryactivity mediated by protease inhibition? J. Ethnophamacol., 113(2), 312-317.
Cavalcanti, A.M., Baggio, C.H., Freitas, C.S., 2006. Safety and antiulcer efficacy studies of Achelleamillefolium L after chronic treatment in Wistar rata. J. Ethnopharmacol., 107(2), 277-284.
Dalsenter, P.R., Cavalcanti, A.M., Andrade, A.J., 2004. Reproductive evaluation of aqueous crude extract of Achilleamille folium L(Asteracea)in wistar rates. Reprod. Toxicol., 18, 819-823.
De lamirande, E., Gagnon, C., 1992. Reactive oxygen species and human spermatozoa.Deplection of adenosine triphosphate plays an important role in the inhibition of sperm motility. J. Androl., 13, 379-386.
De Laszlo, H., Henshaw, P.S., 1954. Plant materials used by primitive people to effect fertility. Sci., 119(3097), 626-
Emami, A., Shams Ardakani, M.R., Mehregan, I., 2004. Encyclopedia of Medicinal Plants. Traditional Medicine and Materia Medica Research Center(TMRC), Shaheed Beheshti University of Medical Sciences. 449.
Garrido, N., Meseguer, M., Simon, C., Pellicer, A., Remohi, J., 2004. Pro-oxidative and anti-oxidative imbalance in human semen and its realtion with male fertility. Asia. J. Androl., 6, 59-65.
Granelli-Piperno, A., 1990. Lymphokine gene expression in vivo is inhibited by Cyclosporin A. J. Exp. Med., 171(2), 533-544.
Kastner, U., Sosa, S., Tubaro, A., Breuer, J., Rucker, G., Loggia, R., 1993. Anti- edematousactivity of sesquiterpene lactone from different taxa of the Achilleamillefoliumgroup. Plant. Med., 59, 669-675.
Masuda, H., Fujihira, S., Ueno, H., Kagawa, M., Katsuoka, Y., Mori, H., 2003. Ultrastructural study on cytotoxic effects of cyclosporine A in spermiogenesis in rates. Med. Electron. Microsc., 36, 193-91.
Saleh, R.A., Agarwal, A., 2002. Oxidative stress and male infertility from research bench to clinical practice. J.
Androl., 23(6), 737-752.

Sanchez de Rojas, V.R., Somoza, B., Ortega, T., 1996. Vadilatory effect in rat aorta of eriodicryol obtained from Saturejaobovata. Plant. Med., 62, 272-4.
Seethalakshmi, L., Flores, C., Khauli, R.B., Diamond, D.A., Menon, M., 1990. Evalation of the effect of experimental cyclosporine toxicity on male reproduction and renal function. Reversal by concomitant human chorionic
gonadotropin administration transplantation. 49, 9-17.
Seethalakshmi, L., Menon, M., Malhotra, R.K., Diamond, D.A., 1987. Effect of cyclosporine A on male reproduction in rats. J. Urol., 138, 991-5.
Sefidkon, F., Jamzad, Z., Barazandeh, M., 2005. Essential oil of Saturejabachtiarica Bunge potential source of carvacrol. Iran. J. Med. Aromat. Plant., 20(4), 425-39.
Sies, H., 1993. Strategies of antioxidant defence. Eur. J. Biochem., 215, 213-219.
Takzare Mortazavi, S.H., Hassanzadeh S., Hosseini, M., 1391. The effect ofalcoholic extractof yarrowon spermatogenesisin rats. Faculty of Tehran University of Medical Sciences. 70(11), 690-684.25.
Tozyo, T., Yoshimura, Y., Sakurai, K., 1994. Novel antitumor sesquiterpenoids in Achilleamillefolium. Chem. Pharm. Bull. (Tokyo)., 42(5), 1096-1100.
Vernet, P., Aitken, R.J., Drevet, J.R., 2004. Antioxidant strategies in the epididymis. Mol. Cell. Endocrinol., 216, 31-39.1516.
Zambrano, E., Rodriguez-Gonzalez, G.L., Guzman, C., Garca-Becerra, R., Boeck, L., Diaz, L., Menjivar, M., Larrea, F., Nathanielsz, P.W., 2005. A material low protein diet during pregnancy and lactation in the rat impairs male
reproductive development. J. Physiol., 563(1), 275-284.