Document Type: Original Article


1 Department of oral biology, faculty of dentistry Trisakti University, Kyai Tapa, Grogol, Jakarta Barat 1140, Indonesia

2 Department of oral biology, faculty of dentistry Yarsi University, Letjend Suprapto, Central Jakarta City, 10510, Indonesia



Chitosan and Calcium are one of the natural polymeric materials obtained from the exoskeleton of Xylotrupes gideon through demineralization, deproteinization and deacetylation processes. Nano chitosan and calcium can inhibit the demineralization process, which is caused by the consumption of acetic acid. This study aims to determine the effect of nano chitosan pretreatment and nano calcium X. gideon on the application of acetic acid toward tooth enamel hardness.
The study used 36 upper teeth premolars divided into 4 groups; the first group was artificial saliva control, the second group was acetic acid application without pretreatment with nano chitosan and nano calcium, the third group was given pretreatment with nano chitosan and the fourth group was given pretreatment with nano chitosan and nano calcium (5:1).
The results showed that there were significant differences in all groups both without or with nano chitosan pretreatment and combination nano chitosan and nano calcium X.gideon on the application of acetic acid to hardness of tooth enamel (p


Main Subjects


Abou Neel, EA, Aljabo, A, Strange, A, Ibrahim, S, Coathup, M, Young, AM, Bozec, L, Mudera, V.

 (2016). Demineralization–remineralization dynamics in teeth and bone. Int. J. Nanomedicine, 6:4743–47.

Arnaud, TMS, De Barros Neto, B, Diniz, FB. (2010). Chitosan effect on dental enamel de-remineralization: An in vitro evaluation. J. dent., 38:848-52.

Budak, NH, Aykin, E, Seydim AC, Greene, KA, Guzel-sydim, ZB. (2014). Functional properties of vinegar. J. Food Sci., 79:757-64.

Changa, J, Chena, C, Ellis, AV, Tung, CH. (2012). Studies of chitosan at different ph’s in the removal of common chlorinated organics from wastewater. Int. J. Appl. Sci. Eng., 10:293-306.

Cortesia, C, Vilchèze, C, Bernut, A, Contreras, W, Gómez, K, De Waard, J, Jacobs, WR, Kremer, L, Takiffa, H. (2014). Acetic acid, the active component of vinegar, is an effective tuberculocidal disinfectant. Am. Soc. Microbiol., 5:1-4.

Ehlen, LA, Marshall, TA, Qian, F, Wefel, JS, Warren, JJ. (2008). ‘Acidic beverages increase the risk of in vitro tooth erosion. Nutr. Res., 28:299–303.

Ghosh, A, Pallavi, SK, Nagpal, B. (2016). Nutrition and oral health, Lambert Academic Publishing, Deutschland, Germany, pp 6-73.

Goldberg, M, Kulkarni, AB, Young, M, Boskey, A. (2012). Dentin: Structure, composition and mineralization: The role of dentin ECM in dentin formation and mineralization. Front Biosci, 3:711–35.

Guiglia, R, Musciotto, A, Compilato, D, Procaccini, M, Lo Russo, L, Ciavarella, D, Lo Muzio, L, Cannone, V, Pepe, I, D'Angelo, M, Campisi, G. (2010). Aging and oralhHealth: Effects in hard and soft tissues. Curr. Pharm. Des., 16:619-30.

Hegde, MN, Sajnani, AR. (2017). Salivary proteins—A Barrier on enamel demineralization: An in vitro study. Int. J. Clin. Pediatr Dent., 10:10-13.

Klaric, E, Rakic, M, Sever, I, Milat, O, Par, M, Tarle, Z. (2015). Enamel and dentin microhardness and chemical composition after experimental light-activated bleaching. Oper Dent., 40:132-41.

Komariah, A, Laksono, WA, Bustami, DA, Trenggono, BS. (2017). Effects Chitosan and Calcium Nanoparticles Mouthwash from Xylotrophes gideon In the Liver And Kidney Rat. Res. J. Pharm. Biol. Chem. Sci., 8:1-9.

Komariah, A, Tatara, RA, Bustami, DA. (2017). Efficacy of Rhinoceros Beetle (Xylotrupes gideon) nano chitosan and calcium mouthwash in reducing quantity oral cavity bacteria among elementary school age children. Int. J. Adv. Biol. Biomed. Res., 5: 41-7.

Lee, H, Tsai S, Kuo, C, Bassania, AW, Pepe-Mooney, B, Miksa, D, Masters, J, Sullivan, R, Composto, RJ. (2012). Chitosan adsorption on hydroxyapatite and its role in preventing acid erosion. J. Coll. Interface Sci., 385:235-43.

Mesquita-Guimarães, KS, Scatena, C, Borsatto, MC, Rodrigues-Júnior, AL, Serra, MC. (2015). Effect of foods and drinks on primary tooth enamel after erosive challenge with hydrochloric acid. Bra. Oral Res., 29:1-7.

Priyadarsini, S, Mukherjee, S, Mishra, M. (2017). Nanoparticles used in dentistry: A review. JOBCR.  Pp. 1-12.

Rahardjo, A, Nugraheni, DDT, Humaira, G, Adiatman, M, Maharani, DA. (2015). Efficacy of toothpaste containing nano calcium in dentin remineralization. Makara J. Health Res., 19:43-7.

Sosa-Puente, C, Solís-Soto, J, Cruz-Fierro, N, López-Villarreal, S, Nakagoshi-Cepeda, S. (2014) Dental erosion: causes, diagnostics and treatment. J. Oral Res., 3:257-61.

Visveswaraiah, PM, Prasad, D, Johnson, S. (2014). Chitosan A novel way to intervene in enamel demineralization; An in vitro study. Int. J. Curr. Microbiol. App. Sci., 3:617-67. 

Visveswaraiah, PM, Prasad, D. (2016). Effect of water soluble carboxymethyl chitosan and chitosan lactate on enamel demineralisation- an sem study. Res. J. Pharm. Biol. Chem. Sci., 7:427-33.

Wongkhantee, S, Patanapiradej, V, Maneenut, C, Tantbirojn D. (2006). Effect of acidic food and drinks on surface hardness of enamel, dentine, and tooth-coloured filling materials. J. dent., 34:214-20.