Document Type: Original Article

Authors

1 1Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural resources, University of Mohaghegh Ardabili, Ardabil, Iran

2 Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural resources, University of Mohaghegh Ardabili, Ardabil, Iran

3 Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Iran

4 Plant Protection Research Department, Ardabil Agricultural and Natural Resources Research and Education Centre, AREEO, Ardabil, Iran

10.33945/SAMI/IJABBR.2020.2.7

Abstract

In order to investigate physiological and biochemical changes of wheat under stress residue of Imazethapyr herbicide (Imazethapyr), an experiment was conducted in a completely randomized design with four replications in greenhouse conditions at university of Mohaghegh Ardebili. The treatments consisted of five dose levels of herbicide Imazethapyr (0, 0.011, 0.022, 0.033 and 0.044 micrograms of herbicide) per kilogram of soil. The analysis of variance showed that the content of the main and auxiliary pigments of leaf, proline, sugar content, protein, activity of catalase enzymes, peroxidase, polyphenol oxidase, root and shoot dry weight were significantly affected by herbicide. The results showed the Imazethapyr herbicide stopped the production of valine leucine and isoleucine amino acids. As a result, the rapid reduction in the volume of these amino acids led to a reduction in protein synthesis in wheat plant. Therefore, the application of doses of 0.011, 0.022, 0.033 and 0.044 micrograms of herbicides decreased 14, 26, 44 and 47 percent of leaf protein content, respectively, compared with control treatment. Further, theapplication of these doses resulted in the reduction of 2.8, 5, 27 and 64% of activity of catalase enzyme, 3.5, 39, 49 and 52% peroxidase enzyme activity and 13, 24, 35 and 46% activity of polyphenol oxidase enzyme, compared with control treatment. The results revealed that Imazethapyr herbicide activates wheat antioxidant enzymes to reduce plant tolerance from damage caused by herbicide residues; therefore, it can be used as a marker or index of herbicide damage rate in physiological research.

Keywords

Abedi, T, Pakniyat, H. (2010). Antioxidant enzyme changes in response to drought stress in ten cultivars of oilseed rape (Brassica napusL.). Czech. J. Genet. Plant., 46:27-34.

Apel, K, Hirt, H. (2004). Reactive Oxygen Species: Metabolism, Oxidative Stress, and Signal Transduction. Annu. Rev. Plant Biol., 55:373–399.

Arora, A, Sairam, RK, Srivastava, GC. (2002). Oxidative stress and antioxidant system in plants. Plant Physiol., 82:1227-1237.

Ashton, FM. (1976). Mobilization of storage proteins of seeds. Ann. Rev. Plant Physiol., 27:95-117.

Barua, AS. (1990). Degradation of pendimethalin by soil fungi. Pest Manag. Sci., 29:419–425.

Basantani, M, Srivastava, A, Sen, S. (2011). Elevated antioxidant response and induction of glutathione S-transferase after glyphosate treatment in Vigna radiata (L.) Wilczek. Pestic. Biochem. Physiol., 99:111–117.

Bates, LS, Waldern, RP, Teare, ID. (1973). Rapid determination of free proline for water-stress studies. Plant Soil., 39:205-207.

Bradford, M. (1976). A rapid and sensitivemethod for the quanititation of microgram quantities of protein utilizing the principle of protein-dye binding. Ann. Biochem., 72:248-254.

Cakmak, I, Horst, WJ. (1991). Effect of aluminum on lipid peroxidation, superoxide dismuatse, catalase, and peroxidase activities in root tips of soybean (Glycine max). Plant Physiol., 83:463-468.

Celen, AE, Avcioglu, R, Geren, H. (2006). Herbage yield of Persian clover (Trifoliumresupinatum L.) as affected by row distance and herbicide application. Crop Protect., 25:496-500.

Damanik, RI. (2012). Response of antioxidant systems in oxygen deprived suspension cultures of rice (Oryza sativa L.). Plant Growth Regul., 67:83-92.

Demiral, T, Türkan, I. (2004). Does exogenous glycinebetaine affect antioxidative system of rice seedlings under NaCl treatment?. J. Plant Physiol., 161:1089–1100.

Dubois, M, Gilles, KA, Hamilton, JK, Rebers, PA, Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Anal. Chem., 28:350-356.

El-Nahas, AI. (2000). Mutagenic potential of imazethapyr herbicide (Imazethapyr®) on Viciafabain the presence of urea fertilizer. Pak. J. Biol. Sci., 3:900-905.

El-Taybe, MA, Zaki, H. (2009). Cytophysiological response of Viciafabato glyphosate-based herbicide. Am. Eurasian J. Sustain. Agric., 2:168–175.

Fruest, EP, Norman, MN. (1991). Interaction of herbicide with photosynthetic electron transport. Weed Sci., 39:458- 463.

Gar’kova, AN, Rusyaeva, MM, Nushtaeva, OV, Aroslankina, YN, Lukatkin, AS. (2011). Treatment with the Herbicide Granstar Induces Oxidative Stress in Cereal Leaves. Russ. J. Plant Physl., 58:1074–1081.

Gaston, S, Zabalza, A, Gonza´lez, EM, Arrese-Igor, C, Aparicio-Tejo, PM. (2002). Imazethapyr, an inhibitor of the branched-chain amino acid biosynthesis, induces aerobic fermentation in pea plants. Physiol. Plantarum., 114:524–532.

Hemaprabh, AG. (2013). Evaluation of drought tolerance potential of elite genotypes and progenies of sugarcane (Saccharum sp. hybrids). Sugar Tech., 15:9-16.

Hoseiny Rad, M, Ashraf Aivazi, A, Jagannath, S. (2011). Cytogenetic and biochemical effects of imazethapyr in wheat (Triticum durum). Turk. J. Biol., 35:663-670.

Jabee, F, Ansari, MYK, Shahab, D. (2008). Studies on the effect of maleic hydrazide on root tip cells and pollen fertility in Trigonellafoenum-graecumL. Turk. J. Bot., 32:337-344.

Jung, S. (2003). Expression Level of Specific Isozymes of Maize Catalase Mutants Influences Other Antioxidants on Norflurazon Induced Oxidative Stress, Pestic. Biochem. Physiol., 75:9–17.

Khalaf, AF, Deya, EMR, Asmaa, KM, Abdelrahman, MA. (2011). Herbicides and salicylic acid applications caused alterations in total amino acids and proline contents of peanut cultivars. J. Environ. Sci., 6:55-61.

Khan, V. (1975). Polyphenol oxide activity and browning of three Avocado varieties. J. Agric. Food Chem., 26:1319-1324.

Khedr, AHA, Abbas, MA, Wahid, AAA, Quick, WP, Abogadallah, GM. (2003). Proline induces the expression of salt-stress responsive proteins and may improve the adaptation of Pancratiummaritimum L. to salt-stress. J. Exp. Bot., 54:2553–2562.

Kumar, S, Arya, SK, Roy, BK. (2010). The effects of 2,4-dichlorophenoxy acetic acid and isoproturon herbicides on the mitotic activity of wheat (TriticumaestivumL.) root tips. Turk. J. Biol., 34:55-66

Langaro, AC, Dirceu, A, Queli, R, Jessica, RG, Lais, TP. (2017). Oxidative stress caused by the use of preemergent herbicides in rice crops. Rev. Cienc. Agron., 48:358-364.

Maria, PR, Аnna, MS, Yevgen, YM. (2014). Decrease of the herbicide fenoxaprop phytotoxicity in drought conditions: the role of the antioxidant enzymatic system. J. Plant Prot. Res., 54:390-394.

McCourt, JA, Pang, SS, King-Scott, J, Guddat, LW, Duggleby, RG. (2005). Herbicide-binding sites revealed in the structure of plant acetohydroxyacid synthase. P. Natl. Acad. Sci., 103:569–573.

Molinari, HBC, Marur, CJ, Daros, E, de Campos, MKF, de Carvalho, JFRP, Filho, JCB. (2007). Evaluation of the stress inducible production of praline in transgenic sugarcane (Saccharum spp.): osmotic adjustment, chlorophyll fluorescence and oxidative stress. Physiol. Plant., 130:218–229.

NematAlla, MM, Badawi, AM, Hassan, NM. (2008). Effect of metribuzin, butachlor and chlorimuron-ethyl on amino acid and protein formation in wheat and maize seedlings. Pestic. Biochem. Physiol., 90:8-18.

Nohatto, MA, Agostinetto, D, Langaro, AC, de Oliveira, C, Ruchel, Q. (2016). Antioxidant activity of rice plants sprayed with herbicides. Pesq. Agropec. Trop. Goiânia., 46:28-34.

 

Okuma, E, Murakami, Y, Shimoishi, Y, Tada, M, Murata, Y. (2004). Effects of exogenous application of proline and betaine on the growth of tobacco cultured cells under saline conditions. Soil Sci. Plant Nutr., 50:1301–1305.

Pang, SS, Guddat, LW, Duggleby, RG. (2003). Molecular basis of sulfonylurea herbicide inhibition of acetohydroxyacid synthase. J. Biol. Chem., 278:7639–7644.

Parrish, SK, Euler, JPR, Grogna, M, Spirlet, A, Walker, F, MacVicar, H, Cullington, JE. (1995). Field, glasshouse and laboratory investigations into the rate of degradation of MON 37500 in European soils. Br. Crop. Protect. Conf. Weeds., 2:667–672.

Peixoto, F, Alves-Fernandes, D, Santos, D, Fonta´nhas-Fernandes, A. (2006). Toxicological effects of oxyfluorfen on oxidative stress enzymes in tilapia Oreochromisniloticus. Pestic. Biochem. Physiol., 85:91-96.

Peng, J, Sun, D, Nevo, E. (2011). Wild emmer wheat, Triticumdicoccoides, occupies a pivotal position in wheat domestication. Aust. J. Crop. Sci., 5:1127-1143.

PI, PY. (2015). Studies on harvest time residue of pyrazosulfuron ethyl, a new generation herbicide, in transplanted rice in the entisols of vellayani, South Kerala, Int. J. Agric. Sci. Vet. Med., 3 (3): 49–54.

Qian, H, Lu, T, Peng, X, Han, X, Fu, Z. (2011). Enantioselective Phytotoxicity of the Herbicide Imazethapyr on the Response of the Antioxidant System and Starch Metabolism in Arabidopsis thaliana. PLoS ONE., 6:1-12.

Radwan, DEM, Fayez, KA, Mahmud, SY, Hamad, A, Lu, G. (2007). Physiological and metabolic changes of Cucurbita pepo leaves in response to zucchini yellow mosaic virus (ZYMV) infection and salicylic acid treatments. Plant Physiol. Biochem., 45:480-489.

Reade, JPH, Cobb, AH. (2002). Herbicides: modes of action and metabolism. In: Naylor R.E.L.(Ed), Weed Management Handbook, 9th ed., Blackwell Science. Oxford. pp. 134-170

Royuela, M, Gonza´lez, A, Gonza´lez, EM, Arrese-Igor, C, Aparicio-Tejo, PM. (2000). Physiological consequences of continuous, sublethal imazethapyr supply to pea plants. J. Plant Physiol., 157:345-354.

Scarponi, L, Martinetti, L, Alla, MMN. (1996). Growth response and changes in starch formation as a result of imazethapyr treatment of soybean (Glycine max L). J. Agr. Food Chem., 44:1572-1577.

Singh, SB, Das, TK, Kulshrestha, G. (2013). Persistence of herbicide fenoxaprop and its acid metabolite in soil and wheat crop. J. Environ. Sci. Heal., 48:320-324.

Song, NH. (2007). Biological responses of wheat (Triticumaestivum) plants to the herbicide chlorotoluron in soils. Chemosphere., 68:1779-1787.

Song, NH, Yang, ZM, Zhou, LX, Wu, X, Yang, H. (2006). Effect of dissolved organic matter on the toxicity of chlorotoluron to Triticumaestivum. J. Environ. Sci., 17:101–108.

Sondhia, S. (2015). Residues of imazethapyr in field soil and plant samples following an application to soybean. Indian J. Weed Sci., 47:166–169.

Sood, A. (2012). Differential responses of hydrogen peroxide, lipid peroxidation and antioxidant enzymes in Azollamicrophyllaexposed to paraquat and nitric oxide. Biologia., 67:1119-1128.

Štajner, D, Popovic, M, Štajner, M. (2004). Herbicide Induced Oxidative Stress in Lettuce, Beans, Pea Seeds and Leaves. Biol. Plant., 47:575-579.

Stettler, M, Eicke, S, Mettler, T, Messerli, G, Hortensteiner, S. (2009). Blocking the metabolism of starch breakdown products in Arabidopsis leaves triggers chloroplast degradation. Mol. Plant., 2:1233-1246.

Wang, SH, Yang, ZM, Lu, B, Li, SQ, Lu, YP. (2004). Copper induced stress and antioxidative responses in roots of Brassica juncea L. Bot. Bull. Acad. Sin., 45:203-212.

Wilcut, JW. (1998). Factors limiting the distribution of cogongrass, Imperatacylindrica, and torpedograss, Panicumrepens. Weed Sci., 36:577-582.

Yang, H, Wu, X, Zhou, LX, Yang, ZM. (2005). Effect of dissolved organic matter on chlorotoluron sorption and desorption in soils. Pedosphere.,15:432–439.

Yin, XL, Lei, J, Ning, HS, Hong, Y. (2008). Toxic Reactivity of Wheat (Triticumaestivum) Plants to Herbicide Isoproturon. J. Agr. Food Chem., 56:4825-4831.

Zabalza, A, Orcaray, L, Gaston, S. (2004). Carbohydrate accumulation in leaves of plants treated with the herbicide chlorsulfuron or imazethapyr is due to a decrease in sink strength. J. Agr. Food Chem., 52:7601-7606.

Zahan, T, Muktadir, MA, Rahman, MM, Ahmed, MM. (2018). Response of the succeeding crops as affected by the residue of herbicides applied in wheat in Old Brahmaputra Floodplain, Bangladesh. J. Agr. Sci., 16:451-457.