Document Type: Original Article

Authors

1 Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

2 Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

3 Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

4 Iranian Center of Neurological Research, Neuroscience Institute, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran

10.33945/SAMI/IJABBR.2020.5.1

Abstract

Background: Postpartum depression (PPD) is one type of major depression that has harmful effects on mother, infant and family relationships. Therefore, this study investigated the protective effects of hydro-alcoholic extract of Nigella sativa on PPD in mice.
Methods: In this experimental study, adult female mice were randomly divided into 6 groups (n=10): control, PPD, Nigella sativa 200, bicuculline, muscimol and fluoxetine. In all animals except in the control group, PPD was induced by progesterone withdrawal. In groups of Nigella sativa 200, bicuculline, muscimol and fluoxetine, mice received 200 mg/kg Nigella sativa, 1 mg/kg bicuculline + 200 mg/kg Nigella sativa, 0.5 mg/kg muscimol and 15 mg/kg fluoxetine, respectively. Then, after 1 hr, the forced swimming test and open field test was examined.
Results: PPD caused significant increases in the immobility times in the forced swimming test (P < 0.05). Administration of Nigella sativa, muscimol and fluoxetine attenuated depression-related behaviors in compared to the PPD group (all P < 0.05). However, combined administration bicuculline with Nigella sativa prevented antidepressant effects of this extract. Moreover, there were no significant differences in the crossing number in the open field test of all groups.
Conclusions: Administration of Nigella sativa hydro-alcoholic extracts can be beneficial to the improvement in PPD and exerts possibly these protective effects partially through increase of gamma amino butyric acid (GABA)-A levels.

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Ahirwar, D, Ahirwar, B. (2020). Antidepressant effect of Nigella sativa in stress-induced depression. Res. J. Pharm. Technol., 13(4):1611-1614.

Arbabi, L, Baharuldin, MTH, Moklas, MAM, Fakurazi, S, Muhammad, SI. (2014). Antidepressant-like effects of omega-3 fatty acids in postpartum model of depression in rats. Behav. Brain. Res., 271:65-71.

Elkhayat, ES, Alorainy, MS, El-Ashmawy, IM, Fat’hi, S. (2016). Potential antidepressant constituents of Nigella sativa seeds. Pharmacogn. Mag., 12:S27-31.

El-Naggar, T, Gomez-Serranillos, MP, Palomino, OM, Arce, C, Carretero, ME. (2010). Nigella sativa L. seed extract modulates the neurotransmitter amino acids release in cultured neurons in vitro. J. Biomed. Biotechnol., 2010:398312.

Gholamnezhad, Z, Shakeri, F, Saadat, S, Ghorani, V, Boskabady, MH. (2019). Clinical and experimental effects of Nigella sativa and its constituents on respiratory and allergic disorders. Avicenna. J. Phytomed., 9(3):195-212.

Ghorbani, F, Rakhshandeh, H, Kazemi, SA. (2012). The effects of Nigella sativa hydro-alcoholic extract and thymoquinone on lipopolysaccharide-induced depression like behavior in rats. J. Pharm. Bioallied. Sci., 4(3):219-225.

Harati, E, Sadeghipour Roodsari, HR, Seifi, B, Kamalinejad, M, Nikseresht, S. (2014). The effect of oral Matricaria Chamomilla extract and selenium on postpartum depression and plasma oxidant-antioxidant system in mice. TUMJ., 71(10):625-634.

Hossain, SJ, Roy, BR, Hossain, AT, Mehrin, F, Tipu, SM, Tofail, F, Arifeen, SE, Tran, T, Fisher, J, Hamadani, J. (2020). Prevalence of Maternal Postpartum Depression, Health-Seeking Behavior and Out of Pocket Payment for Physical Illness and Cost Coping Mechanism of the Poor Families in Bangladesh: A Rural Community-Based Study. Int. J. Environ. Res. Public. Health., 17(13):E4727.

Hosseini, M, Zakeri, S, Khoshdast, S, Yousefian, FT, Rastegar, M, Vafaee, F, Kahdouee, S, Beckley, EH, Finn, DA. (2007). Inhibition of progesterone metabolism mimics the effect of progesterone withdrawal on forced swim test immobility. Pharmacol. Biochem. Behav., 87(4):412-419.

Jahangard, L, Mikoteit, T, Bahiraei, S, Zamanibonab, M, Haghighi, M, Sadeghi Bahmani, D, Brand, S. (2019). Prenatal and postnatal hair steroid levels predict post-partum depression 12 weeks after delivery. J. Clin. Med., 8(9):1290.

Kitamura, Y, Hongo, S, Yamashita, Y, Yagi, S, Otsuki, K, Miki, A, Okada, A, Ushio, S, Esumi, S, Sendo, T. (2019). Influence of lipopolysaccharide on diazepam-modified loss of righting reflex duration by pentobarbital treatment in mice. Eur. J. Pharmacol., 842:231-238.

Meky, HK, Shaaban, MM, Ahmed, MR, Mohammed, TY. (2019). Prevalence of postpartum depression regarding mode of delivery: a cross-sectional study. J. Matern. Fetal. Neonatal. Med., 1-8.

Payne, JL, Maguire, J. (2019). Pathophysiological mechanisms implicated in postpartum depression. Front. Neuroendocrinol., 52:165-180.

Porsolt, R, Bertin, A, Jalfre, M. (1977). Behavioral despair in mice: a primary screening test for antidepressants. Arch. Int. Pharmacodyn. Ther., 229(2):327-336.

Seibenhener, ML, Wooten, MC. (2015). Use of the open field maze to measure locomotor and anxiety-like behavior in mice. J. Vis. Exp., 96:e52434.

Slomian, J, Honvo, G, Emonts, P, Reginster, JY, Bruyere, O. (2019). Consequences of maternal postpartum depression: A systematic review of maternal and infant outcomes. Women's Health., 15:1745506519844044.

Wang, SM, Han, C, Bahk, WM, Lee, SJ, Patkar, AA, Masand, PS, Pae, CU. (2018). Addressing the side effects of contemporary antidepressant drugs: a comprehensive review. Chonnam. Med. J., 54(2):101-12.

Xu, J, Kurup, P, Nairn, AC, Lombroso, PJ. (2018). Synaptic NMDA Receptor Activation Induces Ubiquitination and Degradation of STEP 61. Mol. Neurobiol., 55(4):3096-3111.

Yimer, EM, Tuem, KB, Karim, A, Ur-Rehman, N, Anwar, F. (2019). Nigella sativa L. (black cumin): a promising natural remedy for wide range of illnesses. Evid. Based. Complement. Alternat. Med., 2019:1528635.