Document Type: Original Article


1 Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran

2 Department of Agronomy and plant Breeding, Agriculture Research Center, Zabol University, Zabol, Iran

3 Department of Biotechnology, Faculty of Agriculture, Zabol University, Zabol, Iran


The aim of this study was to evaluate the antimicrobial activities of Trachyspermum ammi, essential oil against different kinds of microorganisms by microtiter plate method. All 36 isolates of (12 Klebsiella pneumoniae, 12 E.coli and 12 Staphylococcus aureus) isolated from urine culture of hospitalized patients (Amir Al-Momenin Hospital, Zabol, south-eastern Iran) suffered from urinary tract infections were evaluated. In this study, the essential oil of Trachyspermum ammi obtained by hydrodistillation for 2.5-3 h using a Clevenger-type apparatus and the minimum inhibitory concentrations were determined to characterize the antimicrobial activities of this essential oil. The results showed E. coli isolates were resistance to 4 of the antibiotics including ceftazidime (50%) cefixime (41.6%), tetracyclin(75%), erythromycin(58.3%). However k. pneumonia isolates were resistance to 3 of the agent including ceftazidime(33.3%) ,cefixime(58.3%), erythromycin(75%) and S.aureus isolates  were resistance to 6 of the agent including cefixime (33.3%), trimethoprim-sulfamethoxazol(41.66%), penicillin(50%), oxacillin(3.3%), ceftazidime(66.6%) and vancomycin(8.3%)  and the MIC value was also determined against all the tested bacteria. The highest MIC values of essential oil were determined 100ppm against E. coli and highest MIC value for K.pneumoniae was 250ppm. In conclusion, it seems that Trachyspermum ammi essential oil could inhibit the growth of all of the tested bacteria.


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