Effect of H2SO4 on Seed Germination and Viability of Canna indica L. Ornamental Plant

Document Type: Original Article


Young Researchers ans Elite Club, Jiroft Branch, Islamic Azad University, Jiroft, Iran


Canna indicaL.roots are used for medicinal purpose. A decoction of the root with fermented rice is used in the treatment of gonorrhea and amenorrhea. The seed of canna is extremely hard, and needs to be "scarified" before sowing. The aim of the present investigation is to determine the hardiness problem of the seed. The seed sample was collected from the IARI, New Delhi in 2008. The work consists of Physical purity, standard germination test, seed vigour test. Experimental results has shown that, seed sample recorded the purity of seed (97.55 %) and seed sample showed the maximum germination percentage 95% after three and four hrs, H2SO4 scarification. The maximum root length (34.07 mm), maximum shoot length (23.62 mm) and maximum seedling shoot and root fresh weight (0.23 and 0.24 g) were observed at three, two hrs and control. H2SO4 scarification. The results indicated that H2SO4 scarification increase the germination percentage but it reduce the viability of the seed.


Babashpour, M and  Sharifivash, R (2010). Effect of acid scarification and hot water on seed germination of honey locust(Gleditschia triacanthos). Proceedings of the Fifth National Conference on New Ideas in agriculture. 27-28 Bahman Months, Islamic Azad University (Isfahan).

Bisland, D.M., Brabdenburg, N.R. and Berlag, A.G (1984). A procedure for evaluating scarification process. J. Appl. Seed Prod. 2: 25-49.

Chandra Joshi, S., Pant, S.C (2010). Effect of H2SO4 on Seed Germination and Viability of Canna indica L. a Medicinal Plant. Journal of American Science. 6(6): 23-26.

Elahifard, E., Montazeri, M., Zand, E and Rashed, M.H (2005). Factors enhancing germination of Phalaris minor seeds obtained from three provinces of Iran. Iranian J. Weed Sci.1: 79-92.

Fang, S., Wang, J., Wei, Z and Zhu, Z (2006). Methods to break seed dormancy in Cyclocaria paliurus(Batal) IIjinskaja. Sci. Hort. 110: 305-309.

Fang, L., Qingfeng, L., Shujun, L and Jun, X (1998). Some methods for stimulating germination of carex seeds. Pratacultural Science, 15(5): 39-43.

Fordham, J (1965). Germination of woody legume seeds with impermeable seed coats. A Continuation of the bulletin of poular information of the Arnold Arboretum, Harvard university, Volume 25 Numer l.

Gebre G.H, Karam N.S (2004) Germination of Cercis siliquastrum seeds in response to gibberellic acid and stratification. Seed Science and Technology 32: 255-260.

Ghadiri, H  and Niazi, M (2005). Effects of scarification and stratification on seed germination and dormancy of Turgenia latifolia, Cuscuta sp. and Sophora alopecuroides in different temperature regimes. J. Iran Agric. Res. 24: 9-17.

Gizachew, L and Scarisbrick, D (1999). Germination of Shamaecytisus palmensis as affected by ageing and method of pre-germination treatment. African Crop Science Journal, 7(2): 165-171.

Hopkins, F.F. (1923). The behavior of hard seeds of certain legumes when subjected to conditions favorable to germination. Proc. Assoc. off. Seed Analysts, N. Amer, 14: 46-48.

Herron, H and Clemens, J. (2001). Seed dormancy and germination in Melicytus ramiflorus (violaceae). New Zealand Journal of Botany. 39:245-249.

Jones R.O, Geneve R.L (1995) Seedcoat structure related to germination in eastern redbud (Cercis canadensis L.). Journal of the American Society for Horticultural Science 120: 123-127.

Khaleghe, A and Dehghan. Mollami, N (2009). Effects of sulfuric acid and hot water treatments on seed germination index and Acacia Sour Hindi, Iranian Journal of Horticultural Science, 3: 20-27.

Mahmood Zadeh, A., Nojavan, M and Bagheri, Z (2002). The effect of different treatments for breaking dormancy and germination stimulation in Melilotus officinalis L. J. Gorgan Agric. and Environ. Sci. 10: 55-63.

Mirzadeh vaghefi, S., Sadat Jamzad, Z., Jalile, A and Nasiri, M (2009). Vtshdyd break dormancy and germination in three species of hawthorn (C. rataeguspersica, C. Aminii, C. babakhanloui), Journal of Forest and Poplar Research, 38: 544-559.

Nassiri, M and Isavand, H (2001). Seed dormancy and germination  Brshkstn woman of sulfuric asrasyd khsb night (Albizia julibrissin Durazz) and carob (Ceratonia siliqua L.). Journal of Plant Breeding and Genetics Research Vjngly of pasture, 8: 95-111.

Nikolaeva, M.G (1969). Physiology of deep dormancy in seeds. IPST Press, Jerusalem, 220 pp.

Noorafkan, H and Khoshkhui, M (2005). Investtgations on sexual propagation of golden rain (Koelreuteria paniculata laxm.) and chinese rain(k. elegans seem.) trees. Journal of Horticultural Science and Technology, 6 (2): 98-89.

Ortega Baes, P., De Viana, M. L and Sühring, S (2002). Germination in Prosopis ferox seeds: effects of mechanical, chemical and biological scarificators. J. Arid Environ. 50: 185-189.

Olmez, Z., Gokturk, A and Temel, F (2007). Effects of Cold Stratification, Sulphuric Acid, Submersion in Hot and Tap Water Pretreatments on Germination of Bladder-Senna (Colutea armena Boiss. & Huet) Seeds, Seed Science and Technology, 35(2): 266-271.

Rascio N, Mariani P, Vecchia FD, Rocca NL, Profumo P, Gastaldo P (1998) Effects of seed chilling or GA3 supply on dormancy breaking and plantlet growth in Cercis siliquastrum L. Plant Growth Regulation 25: 53-61.

Rolston, M.P (1978). Water impermeable seed dormancy. Bot. Rev. 44: 365-396.

SAS. (2002). SAS/STAT® user’s guide, SAS Institute, Cary, NC, USA. 9.1.