Document Type : Original Article

Author

• Seham Mansour Ismail

Insect Population Toxicology Department, Central Agricultural Pesticides Laboratory, Agriculture Research Center, 12618, Giza, Egypt

Abstract

Background: Chlorantraniliprole and emamectin benzoate have been widely used to control pests including FAW. For effective pest control, the sublethal effects of these insecticides should be considered.
Methods: The toxicity of chlorantraniliprole and emamectin benzoate to third-instar FAW larvae has been determined using topical application. Alpha esterase (EST-α) and beta esterase (EST-β) activity were also assessed after 1, 6, 12, 24, and 48 h of exposure of larvae to low doses of these insecticides.
Results: The susceptibility of FAW larvae to chlorantraniliprole and emamectin benzoate increased after 48 h, and the LD₅₀ was 0.18 and 0.23 μg/larvae, respectively.
EST-α and -β activity changed significantly in FAW larvae over time after exposure to insecticides. After only 1 h, EST-α, and -β activity after exposure of larvae to insecticides at LD₁₀ showed no significant difference compared to control. However, under LD₃₀ treatments, the activity of EST-α and -β decreased significantly. Significant reduction of EST-α and -β activity was observed in larvae after 48 h exposure to chlorantraniliprole at LD₃₀ (0.5 and 0.15 nmol/min/mg protein, respectively) followed by emamectin benzoate (1.19 and 0.29 nmol/min/mg protein, respectively) compared with control activity.
Conclusion: Sublethal doses of chlorantraniliprole and emamectin benzoate affected activities of EST-α and -β of FAW.

Graphical Abstract

Keywords

• Alpha esterase
• Beta esterase
• Chlorantraniliprole
• Emamectin benzoate
• Spodoptera frugiperda
• Sublethal effects

Main Subjects

• Biochemistry

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