Document Type: Original Article


Department of Crop Protection, Faculty of Agriculture, University of Jiroft, Jiroft, Iran


Pasteuria penetrans is a bacterial parasite of some plant parasite of nematodes whichcan attach to the cuticle of nematode. Differences in attachment of P. penetrans spores to the second stag juvenile (J2) cuticle may provide evidence for nematode cuticle diversity even between different ages of J2s. For investigating this purpose, we studied the effect of different ages of the same population of Meloidogyne chitwoodi second stage juveniles (J2) on the attachment of a specific P. penetrans spores. Each 200 freshly hatched, 7 and 12 day old J2 were exposed to 40000 spores of P. penetrans. The J2s-spores suspension were centrifuged and were examined with a microscope and attached spores were counted. There were significant differences (P<0.05) in spore attachment between fresh M. chitwoodi J2 and 12-day-old J2. Although there were no significant differences between spore attachment using fresh J2 and 7day-old J2, the numbers of spores attached to the 7-day-old J2 were lower than the numbers attaching to the fresh ones. From this study we can suggest that age of J2s can affect on spores attachment and this may help to have a successful management program


Akhkha A, Kusel J, Kennedy M and Curtis R.H.C (2002). Effects of phytohormones on the surfaces of plant parasitic nematodes. Parasitology 125:165-175.

Barrett J, Wright D.J (1998). Intermediary metabolism. In: Perry, R.N. and Wright, D.J. (eds) The Physiology and Biochemistry of Free-living and Plant-parasitic.

Bert M. Zuckerman and Itzhak Kahane (1983).Caenorhabditis elegans: stage specific differences in cuticle surface carbonhydrates. Journal of Nematology 15(4): 535-538.

Bird A.F, Bird J (1991). The structure of nematodes (2nd edition). Academic Press Inc. New York, pp. 316.

Davies K G, Laird V, Kerry B.R (1991). The motility, development and infection of Meloidogyne incognita encumbered with spores of the obligate hyperparasite Pasteuria penetrans. Revue de Nematologie 14: 611-618.

Davies K.G, Fargette M, Balla G, Daudi A, Duponnois R, Gowen S.R, Mateille T, Phillips M.S, Sawadogo A, Trivino C, Vouyoukalou E, Trudgill D.L (2001). Cuticle heterogeneity as exhibited by Pasteuria spore attachment is not linked to the phylogeny of parthenogenetic root-knot nematodes (Meloidogyne spp.). Parasitology 122: 111-120.

Decraemer W, Karanastasi E, Brown D, Backeljau T (2003). Review of the ulterastructure of nematode body cuticle and its phylogenetic interpretation. Biological Review. 78: 465-510.

Eisenback J. D, and Triantapjyllou H. H (1991). Root-knot nematodes: Meloidogyne Species and Races.
in W. R. Nickle, (ed). A Manual of Agricultural Nematology. Marcel Dekker, Inc., New York, NY, USA, Pp. 191-274

Hewlett T.E, Dickson D.W (1994). Endospore attachment specificity of Pasteuria penetrans from a
peanut field in Florida. Journal of Nematology 26: 103-104.

Hooper D.J (1986). Extraction of free-living stages from soil. In: Southey, J.F. (Ed.), Laboratory methods for work with plant and soil nematodes. MAFF, London, pp. 5-30.

Lopez M, Modha J, Roberts C, Curtis R.H.C, Kusel J (2000). Observations of the changes of the surface cuticle of parasitic nematode using fluorescent probes. Parasitology 120: 203-209.

Lopez M, Modha J, Roberts C, Curtis R.H.C, Kusel J (2000). Observations of the changes of the surface cuticle of parasitic nematode using fluorescent probes. Parasitology 120: 203-209.

O’Bannon J.H, Santo G.S, Nyczepir A.P (1982). Host range of the Columbia root-knot nematode. Plant Disease 66: 1045-1048.
Santo G.S, O'Bannon J.H, Finley A.M, Golden A.M (1980). Occurrence and host range of a new root knot nematode (Meloidogyne chitwoodi) in the Pacific Northwest. Plant Disease 64: 951-952.

Spiegel Y and McClure M.A (1995). The surface coat of plant parasitic nematodes: chemical composition, origin, and biological role - a review. Journal of Nematology 27: 127-134.

Trudgill D.L, Bala G, Blok V.C, Daudi A, Davies K.G, Gowen S.R, Fargette M, Madulu J.D, Mateille T, Mwageni W, Netscher C, Phillips M.S, Sawadogo A, Trivino C.G. Voyoukallou E (2000). The importance of tropical root-knot nematodes (Meloidogyne spp.) and factors affecting the utility of Pasteuria penetrans as a biocontrol agent. Nematology 2: 823-845.