Document Type: Original Article

Authors

1 Department of Pharmacology and Therapeutics, College of Health Sciences, Enugu State University of Science and Technology, Parklane, Enugu, Nigeria

2 Department of Pharmacology and Therapeutics, Faculty of Clinical Medicine, College of Health Sciences, Ebonyi State University, Abakaliki, Nigeria

Abstract

Objective: Annona senegalenesis Pers is a sprawling shrub belonging to the family Annonaceae, and is mainly used in ethnomedicine. The use of decoction of A. senegalenesis is very prominent in the management of various conditions ranging from bacterial infection, worm infestation,  snake bites, pyrexia and febrile convulsion in folk medicine. Methods: The investigation on anticonvulsant activities of A. senegalenesis was undertaken using animal models with a view to elucidating its mechanism of action Results: Results revealed that the aqueous extract of the root of A. senegalenesis was safe at high doses (LD50 954.99±2.86 mg/kg body weight), protected drug–induced convulsion in mice, prevented electroshock in mice, being more effective against generalized than partial seizures and prolonging drug-induced sleep in mice. We conclude that the roots of A. senegalenesis have definite anticonvulsant activity and there is valid pharmacological basis for employing same for this purpose by the local people.

Keywords

Abubakar M.S., Musa A.M., Ahmed A., Hussaini I.M., 2007. The perception and practice of traditional medicine in the treatment of cancers and inflammations by Hausa and Fulani tribes of northern Nigeria. Journal of Ethnopharmacology. 111, 625-629.
Adzu B., Amos S., Adamu M., Gamaniel K., 2003. Antinociceptive and anti-inflammatory effects of the methanol extract of Annona senegalnesis root bark. Journal of Natural Remedies. 3, 63-67.
Adzu B., Abubakar M.S., Izebe K.S., Akumka D.D., Gamaniel K.S., 2005. Effect of Annona senegalenesis root bark extracts on Naja nigricollis nigricollis venom in rats. Journal of Ethnopharmacology. 96, 507-513.
Ajibesin K.K., Ekpo A.B., Bala D.N., Essien E.E., Adesanya S.A., 2008. Ethnobotanical survey of Akwa Ibom State of Nigeria. Journal of Ethnopharmacology. 115, 387-408.
Corda M.G., Giorgi O., Longoni B., Orlandi M., Biggio G., 1990. Decrease in the function of aminobutyric acid coupled chloride channel produced by repeated
administration of pentylenetetrazole in rats Journal Neurosciences. 55, 1216-1221.
Dalziel J.M., 1948. The Useful Plants of West Tropical Africa. Crown Agents for Overseas Governments and Administrations, London.
Diskshit S.K., Tewan P.V., Dixit S.P., 1972. Anticonvulsant activity of Causora delussata. Indian Journal of Physiology and Pharmacology. 16, 81-82.
Duarte F.S., Marder M., Hoeller A.A., Duzzuoni M., Menuless B.G., Pizzolati M.G., Delina T.C.M., 2008. Anticonvulsant and anxiolytic-like effects of compounds isolated from Polygala sabulosa (Polygalaceae) in
rodents: in vitro and in vivo interactions with benzodiazepine binding sites. Psychopharmacology. 197, 351-360.
Irvine F.R., 1961. Woody Plants of Ghana. Oxford Univeristy Press, London.
Jusko W.J., 1977. Bioavailability and disposition kinetics of phenytoin in man, In: Quantitative Analytic Studies in Epilepsy. Kellyway P., Peterson I. (Eds), Raven Press, pp 200-241.
Marini-Bettolo G.B, Necoletti M., Patamia M., Geleffi C., Messana I., 1981. Plant screening by chemical and chromatograghic procedures under field conditions. Journal of Chromatography. 213, 113-127.
Nadkarni K.M., 1985. Medicinal Plants of India. Indian Council of Medicinal Plants Research, New Delhi. Nisar M., Khan I., Simjee S.U., Gilani A.H., Obaidulla P.H., 2008. Anticonvulsant, analgesic and antipyretic activities
of Taxus wallichiana Zucc. Journal of Ethnopharmacology. 116, 490-494.
Ogbadoyi E.O., Abdulganiy A.O., Adama T.Z., Okogun J.I., 2007. In Vivo trypanocidal activity of Annona senegalnesis Pers leaf extract against Trypansome brucei . Journal of Ethnopharmacology. 112, 85-89.
Ojewole J.A.O., 2005. Analgesic and anticonvulsant properties of Tetrapleura tetrapleura (Taub) (Fabiacaea) fruit aqueous extract in mice. Phytotherapy Research. 19, 1023-1029.
Parmar S.S., Dwivedi C., Ali B., Misra R.S., 1972. Determination of the anticonvulsant activity of substituted aminobenzylidnes. Journal of Medicinal
Chemistry. 15:1846-1849.
Porter R.J., Meldrum B.S., 2004. Antiepileptic drugs, In: Katzung B. G. (Ed.) 9th ed., Basic and Clinical Pharmacology. Lange Medical Book, California, pp 219-228.
Wager H., Bladt S., 2001. Plant Drug Analysis: Thin Layer Chromatography, 2nd ed., Springer Londres.
Wambebe C., 1985. Influence of some agents that affect 5-hydroxytryptamine metabolism and receptors on nitrazepam-induced sleep in mice. British Journal of Pharmacology. 84, 185-191.
Zimmerman M., 1983. Ethical guidelines for investigation of experimental pain in conscious animals. Pain. 16, 109-110