International Journal of Advanced Biological and Biomedical Research

Investigating the Expression Levels of Glutathione Peroxidase and Glutathione Reductase Genes in Mastectomies Women

Parmiss Adyani Kalvanagh; Yousef Adyani Kalvanagh

Volume 11, Issue 3 , July 2023, , Pages 115-126

https://doi.org/10.22034/ijabbr.2023.1999566.1433

Abstract

Introduction: Important genes that may be expressed in women candidates for mastectomy due to breast cancer are important; The expression of these genes in these women can play an important ... Read More Introduction: Important genes that may be expressed in women candidates for mastectomy due to breast cancer are important; The expression of these genes in these women can play an important role in the treatment of these patients; The aim of this study was investigating the level of expression of glutathione peroxidase and glutathione reductase genes in mastectomies women.Procedure: After cell culture, the cell suspension was planted in 96-well microplates and treated for 24 hours in a CO2 incubator at 37°C. In order to synthesize cDNA, extracted RNA molecules were used with the help of Fermentaz Revert Aid TM kit, produced in the United States.Results: The concentration of 500 micrograms of nanoparticles caused the death of more than 60% of MCF 7 cells (P< 0.001) while there was no significant difference in all concentrations of nanoparticles on the normal HEK293 cell line .Conclusion: The anti-tumor effects of zinc oxide nanoparticles were shown in increasing the expression of glutathione peroxidase and glutathione reductase genes in MCF-7 breast cancer cell line of mastectomy candidates.Introduction: Important genes that may be expressed in women candidates for mastectomy due to breast cancer are important; The expression of these genes in these women can play an important role in the treatment of these patients; The aim of this study was investigating the level of expression of glutathione peroxidase and glutathione reductase genes in mastectomies women.Procedure: After cell culture, the cell suspension was planted in 96-well microplates and treated for 24 hours in a CO2 incubator at 37°C. In order to synthesize cDNA, extracted RNA molecules were used with the help of Fermentaz Revert Aid TM kit, produced in the United States.Results: The concentration of 500 micrograms of nanoparticles caused the death of more than 60% of MCF 7 cells (P< 0.001) while there was no significant difference in all concentrations of nanoparticles on the normal HEK293 cell line .Conclusion: The anti-tumor effects of zinc oxide nanoparticles were shown in increasing the expression of glutathione peroxidase and glutathione reductase genes in MCF-7 breast cancer cell line of mastectomy candidates.

The effects of chitosan nanoparticles on genes expression of artemisinin synthase in suspension culture of Artemisia annua L: A comparative study

Bita Ghassemi; Fatemeh Dehghan Nayeri; Ramin Hosseini

Volume 9, Issue 2 , June 2021, , Pages 214-227

https://doi.org/10.22034/ijabbr.2021.523524.1343

Abstract

Background: Despite significant efforts, the artemisinin-based drugs are still very expensive due to the limited production of this metabolite within wild Artemisia spp . plants. Therefore, ... Read More

Study the effect of indomethacin administration on breast tumor growth and iNOS gene expression in tumor-bearing mice

Soheil Afshari Behbahanizadeh; Samad Farashi Bonab

Volume 7, Issue 2 , June 2019, , Pages 160-172

https://doi.org/10.33945/SAMI/IJABBR.2019.2.7

Abstract

Breast cancer is a public health concern among the women. iNOS is stated by the effect of various inflammatory factors and is thus called inducible NOS. Investigating iNOS expression ... Read More

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Keywords = Gene Expression

Investigating the Expression Levels of Glutathione Peroxidase and Glutathione Reductase Genes in Mastectomies Women

Abstract

The effects of chitosan nanoparticles on genes expression of artemisinin synthase in suspension culture of Artemisia annua L: A comparative study

Abstract

Study the effect of indomethacin administration on breast tumor growth and iNOS gene expression in tumor-bearing mice

Abstract