Document Type : Short communication


1 Department of Agronomy,Faculty of Agriculture, University of Zabol, Zabol, Iran

2 Zabol University of Medical Sciences, Zabol, Iran

3 Department of Plant Breeding and Biotechnology, University of Zabol, Zabol, Iran

4 Zabol Medicinal Plant Research Center, Zabol University of Medical Sciences, Zabol, Iran


Objective: Infections are increasing caused by antibiotic-resistant Pseudomonas aeruginosa day by day. For this reason, many researchers have tried to find new compounds as alternative antibiotics. The purpose of this study was to evaluate the effect of yarrow extract, green tea and Ajowan  on Pseudomonas aeruginosa. Methods: Extraction  of Achillea, green tea and Ajowan was done with rotary. For this study, 12 strains of Pseudomonas aeruginosa isolated from patients in the city of Zabol. Minimum inhibitory concentrations of the plant extract of Achillea, green tea and Ajowan was determined in different concentrations by dilution on bacteria with plate method. Results: Results from plant extracts showed the highest MIC (minimum inhibitory concentration) of green tea extract was concentration of 2.5 milligrams per milliliter for Pseudomonas aeruginosa that 8 strains were inhibited at this concentration. Also, The highest inhibitory concentration (MIC) of achiella extract against strains of Pseudomonas aeruginosa was 5 milligrams per milliliter that four strains were inhibited at this concentration, the lowest concentration of inhibitor was 62 milligrams per milliliter. Discussion: This study showed that Achiella, green tea and Ajowan have antibacterial effect against strains of Pseudomonas aeruginosa. However, the clinical use of these plants require more and wider research.


Sekiguchi J, Asagi T, Miyoshi-Akiyama T, Kasai A, Mizuguchi Y, Araake M. Outbreaks of multidrug resistant Pseudomonas aeruginosa in community
hospitals in Japan. Journal of clinical microbiology. 2007;45(3):979-89.
Siarkou V, Vitti D, Protonotariou E, Ikonomidis A, Sofianou D. Molecular epidemiology of outbreakrelated Pseudomonas aeruginosa strains carrying the
novel variant blaVIM-17 metallo-‚-lactamase gene. Antimicrobial agents and chemotherapy. 2009; 53(4):1325-30.
Mabberley D. The Plant-Book, 2nd ed. Cambridge University Press, Cambridge. . 1997.
Taylor A, Francis M. Final report on the safety assessment of Yarrow (Achillea Millefolium) extract. International journal of toxicology. 2001;20(2):79-
Benedek B, Kopp B. Achillea millefolium L. s.l. revisited: Recent findings confirm the traditional use. Wiener Medizinische Wochenschrift. 2007;157:312-4.
Smelcorevic A, et al. LC/MS analysis of the essentialoils of Achillea millefolium and Achilleacrithmifolia. Chromatographia. 2010;71:113- 6.
7. Agalakshmi G, Shankaracharya N, Puranaik J. Studies on chemical and technological aspects of ajowan (Trachyspermum ammi) syn (Cerum
copticum Hiren) seeds. J of Food Sci Techno. 2000;37:277- 81.
Carmen C, Reyes A, Rafael G. Beneficial effects of green tea - a review. J AM Coll Nutr. 2006;25(2):79-99.
Mepur H, Thiruverkadu S, CMcClarence, Naftali P, Xing Y, Senthamil R. Epicatechins purified from green tea (Camellia sinensis) differentially suppress growth of genderdependent human cancer cell lines. Evid Based Complement Alternat Med. 2006;3(2):237- 47.
Aktug S, Karapikar M. Inhibition of food borne pathogens by thymol, eugenol, menthol and ethanol. International Journal of Food Microbiology. 1987;4:161-6.
Bown D. The royal horticultural society encyclopedia herbs theiruses.London: Dorling Kindersley Distributed by Houghton Mifflin1996:363-4.
Murthy P, Borse B, Khanum H, Srinivas P. Inhibitory effects of Ajowan (Trachysperum ammi) ethanolic extract on A.ochraceus growth and ochratoxin
production. Turk J Biol. 2009;33:211-7.
Zadeh NY, Ungh A, Mardani K, Khalily M. Molecullar identification , pattern of resistance antibiotic E. coli Shiga toxin-producing and antibacterial activity of
essense of thyme and Ajowan against them. Journal Urmia Medical Sciences. 201122(3).
Tukmechi A, Malakinejad H, gilani BB, Ebrahimi H. Antibacterial activity of Zataria moltiflora and Carum copticum essential oil on Aeromonas hydrophilla.  Guilan: 11th Iranian Microbiology Congress. 2010:35.
Abroman P, Motaghiyan Z, Sharifiyan A, Larijani K. Effect of extraction method on chemical composition and antimicrobial activity of essential oils extracted
copticum. . Food Science and Nutrition. 2010 2:in farsi.
LaGow B. PDR for herbal medicine. 3rd ed. USA: Thamson. 2005;79899-901.
Issabeagloo E, Taghizadieh M, Abri B. Antimicrobial effects of yarrow (Achillea millefolium) essential oils against Staphylococcus species. African Journal of
Pharmacy and Pharmacology 2012;6(41):2895-9.
Salvagnini F, Migliato K, Isaac V, Correa M, Salgado H, Pietro R. Evaluation efficacy of preservatives associated With a achillea millefolium L extract
against Bacillus subtilis. Brazilian Journal of Microbiology. 2006;37:75-7.
Orujaliyan F, R Kasri Kermanshahi. Botanochemical and antibacterial properties of essential oil of yarrow Achillea eriophora DC Shirazi microdilution method. Journal of Horticulture. 201024(1):115-09.
Kermanshah H, Kamangar S, Arami C, Mirsalehiyan A, Kamalinejad M, Karimi M, et al. In vitro evaluation of antibacterial effect of ethanol extracts of sage and yarrow against cariogenic microorganisms Islamic Society of Dental Dentists 2009;21(3):215-20.