International Journal of Advanced Biological and Biomedical Research

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Histochemical Evaluation of the Activities of Vitamin C on Ethanol Administration in Rat; Implication on the Cytoarchitectural and Some Neurochemical Indices of the Prefrontal Cortex

Document Type : Original Article

Authors

A.O. Adekeye 1
C.O. Akintayo 2
L.A. Enye 1
O.O. Ogendengbe 1
A.I. Adeniyi 3

1 Department of Anatomy, College of Medicine and Health Sciences, Afe Babalola University, Ado Ekiti, Nigeria

2 Department of Physiology, College of Medicine and Health Sciences, Afe Babalola University, Ado Ekiti, Nigeria

3 Department of Physiology, College of Medicine and Health Sciences, Ahmadu Bello University, Zaria, Nigeria

Abstract
Objective: This study was to evaluate the effect of Vitamin C on the histology and histochemistry of the prefrontal cortex of ethanol-induced rats. Methods: Male Sprague-Dawley rats were used for the study. Ethical approval was obtained from the University’s ethical committee. The rats were randomly divided into 6 groups of 10 rats each. Rats in group A= free access to normal saline. Rats in group B= treated with 4.25 ml ethanol. Rats in group C= treated with 100 mg/kg Vit. C. Rats in group D= pre-treated with 100 mg/kg Vit. C followed by 4.25 ml ethanol. Rats in group E=co-treated with 100 mg/kg of Vit. C and 4.25ml ethanol.  Rats in group F=post-treated with 4.25ml ethanol followed by 100 mg/kg Vit.C. 24hrs after the last administration, the rats were sacrificed by cervical dislocation: the fraction of the brain for tissue histochemistry was fixed in formol calcium and later processed for Heamotoxylin and Eosin with Cresyl fast violent staining techniques and the other fraction meant for enzyme and/or marker histochemistry was processed accordingly for some neurochemical indices for oxidative stress. Results: The markers of oxidative stress were statistically increased in the rats in group D, E and F compared with the rats in group B. There is a significant reduction of TBARS when compared with ethanol induced group (group B). The histological profile of the prefrontal cortex of rats in group A and C were preserved while that of the rats in group B displayed distorted cytoarchitecture profile with a marked increase in apoptotic bodies, lateral deviation of neurons and a marked increase in the activities of oxidative markers.  

Keywords

Ethanol
Apoptosis
Oxidative markers
Prefrontal cortex
Neurodegeneration
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International Journal of Advanced Biological and Biomedical Research
Volume 3, Issue 3 - Serial Number 3
September and October 2015
Pages 237-240

  • Receive Date 05 September 2015

Article View 11,269
PDF Download 15,209

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