Document Type : Original Article
Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University,Lahijan, Iran
Objective: Aflatoxin G1 and G2, are highly toxic and carcinogenic secondary metabolites produced by Aspergillus flavus and Aspergillus parasiticus fungi sampling was done according to "CBS" instructions from indoor and outdoor stations. Methods: In HPLC measuring, we first purified our sample of all Aflatoxins, We used imonoaffinty columns, Finally the device compared average of the results of the samples curve areas with average of the results of standard curve areas and showed the effective material value based on ppb. Geographical distribution of 107 mentioned samples and The frequency of subgenuses in different regions, The minimum, average and maximum amount of toxins, The average amount of produced toxin in culture medium were determined. Results: In comparison, the average of aflatoxin G1 and G2with HPLC method in biomass the highest examined amount is in the range of -0/5 -0/5 ppb which is produced by fungi such as A. niger, A. flavus, A. fumigatus. In biomass, the correlation between aflatoxin G1 and G2 measured by HPLC method isn’t any reverse relation and meaningful numeral differences. In culture medium, checking the average value of aflatoxin G1 and G2which is measured by HPLC method, we can see a correlation and there is a meaningful numeral difference.
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