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The Production Pattern of Aflatoxin G1,G2 ,by HPLC
Document Type : Original Article
Authors
Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University,Lahijan, Iran
Abstract
Objective: Aflatoxin G1 and G2, are highly toxic and carcinogenic secondary metabolites produced by Aspergillus flavus and Aspergillus parasiticus fungi sampling was done according to "CBS" instructions from indoor and outdoor stations. Methods: In HPLC measuring, we first purified our sample of all Aflatoxins, We used imonoaffinty columns, Finally the device compared average of the results of the samples curve areas with average of the results of standard curve areas and showed the effective material value based on ppb. Geographical distribution of 107 mentioned samples and The frequency of subgenuses in different regions, The minimum, average and maximum amount of toxins, The average amount of produced toxin in culture medium were determined. Results: In comparison, the average of aflatoxin G1 and G2with HPLC method in biomass the highest examined amount is in the range of -0/5 -0/5 ppb which is produced by fungi such as A. niger, A. flavus, A. fumigatus. In biomass, the correlation between aflatoxin G1 and G2 measured by HPLC method isn’t any reverse relation and meaningful numeral differences. In culture medium, checking the average value of aflatoxin G1 and G2which is measured by HPLC method, we can see a correlation and there is a meaningful numeral difference.
Keywords
Subjects
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Vahidi, M.Chaichi ,A.Faezi , M.Modiri ,2012 l.comparativestudy on aflatoxins G1,B1 production patterns in cell extracts of aspergillus in Northern Iran.
Mehdikar , M.Chaichi ,A.Faezi , M.Modiri , 2011. A comparative analytical survey on pattem properties of Aflatoxin B production in the asperigilli Regarding to Iranian Northern States S ubspecies Circumdati .
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Nasir,M.S, Jolly, M.E, 2002.Development of a fluorescence polarization assay for determination of Aflatoxins in tahini . J. Agric. Food chem ,50:3116-3121.
Nilufar , D, Boyacioglu,.D ,2002. Comparative study of these different methods for the determination of Aflatoxin in tahini. J. Agric. Food chem , 50:3375-3379.
Patey ,A, l, Sharman ,M, Wood, R , Gilbert,J , 1989. Determination of aflatoxin concentrations in peanut butter by enzyme-linked immune- sorbent
assay(ELISA):Study of three commercial ELISA kits . J. Food Microbiol ,83:219-225.
Seitz ,L.M., 1975.Comparison of method of aflatoxin analysis by hich pressure liquid chromatography . J.chromatogr ,104:81-91.
Stroka , J, Anklam , E, 2000 . Development of a simplified densitometer for the determination of aflatoxins by thinlayer chromatography.J.chromatogr .A , 904:263-268.
Whitaker ,T, Horwitz ,w, Albert,R., neshim , S, 1996.Variability associated whit analytical methods used to measure aflatoxin in agricultural commodities .J .Assoc. Off. Anal. chem , 79:476- 485.
Craufurd P.Q. , Prasad P.V.V. , Waliyar F. and taheri .A. , 2006.Drought , pot , pre-harrst Aspergillus infection and aflatoxin contamination on peanut in niger .field cropresearch , 98,20-29.
Gupta A , Gopal M , 2002.Aflatoxin production by Aspergillus Havusisolates pathogenic to coconut insect pests . world j, microbial,Biotechnol, 18,325-331.
Ayejuyoo.o , Williams A. B, (2008) . ImafidonT.F. ocratoxin , A burdens in Rice from lagosmarkels. Nigeria , J of Environ .sci , Techpol ,1(2)80-84.
Chiou , C.H , miller , M., Wilson , D.l , Trail , E , linz, J.E , 2002 . chromosomad location plays a role in regulation of aflatoxin gene expression in Aspergillus parasiticus .Appl,Environ , microbial., 38,306-375.
Gunterus A , Rozel.l , Beaudry R.and Linz J.E , (2002).Ethylene inhibits Aflatoxin biosynthesis in aspergillus flavus . parasiticus and aflatoxin in peanuts ,y.AOAC int, 85,911-916.
Bhat RV, Vasanthi S, 2003. Mycotxin food safty risk in developing contries , food . Agriculture and Enuironment.Khurgami , M.Chaichi ,A.Faezi ,M.Modiri ,
2012.comparativestudy on aflatoxins G1,B1 production patterns in culture medium of aspergillus in Northern Iran.
Vahidi, M.Chaichi ,A.Faezi , M.Modiri ,2012 l.comparativestudy on aflatoxins G1,B1 production patterns in cell extracts of aspergillus in Northern Iran.
Mehdikar , M.Chaichi ,A.Faezi , M.Modiri , 2011. A comparative analytical survey on pattem properties of Aflatoxin B production in the asperigilli Regarding to Iranian Northern States S ubspecies Circumdati .
American Association for cancer,1980.An evaluation of chemicals and industrial processes associated whit cancer in humans based on human and animal data:IARC monographs volumes1 to 20.cancer Res ,40(1):1-2.
Cop pock , W.R.& Christian , R.G,2007.Aflatoxins,in:Veterinary Toxicology-Basic and clinical principles,R.C.Gupta , Academic press ,ISBN
0123704677,Sandiego. pp. 939-950.
Eaton DL, Gallaghar EP , 1994. Mechanisms of aflatoxin carcinogenesis . Annu Rev Pharamacol Toxicol,34:135-172.
Nasir,M.S, Jolly, M.E, 2002.Development of a fluorescence polarization assay for determination of Aflatoxins in tahini . J. Agric. Food chem ,50:3116-3121.
Nilufar , D, Boyacioglu,.D ,2002. Comparative study of these different methods for the determination of Aflatoxin in tahini. J. Agric. Food chem , 50:3375-3379.
Patey ,A, l, Sharman ,M, Wood, R , Gilbert,J , 1989. Determination of aflatoxin concentrations in peanut butter by enzyme-linked immune- sorbent
assay(ELISA):Study of three commercial ELISA kits . J. Food Microbiol ,83:219-225.
Seitz ,L.M., 1975.Comparison of method of aflatoxin analysis by hich pressure liquid chromatography . J.chromatogr ,104:81-91.
Stroka , J, Anklam , E, 2000 . Development of a simplified densitometer for the determination of aflatoxins by thinlayer chromatography.J.chromatogr .A , 904:263-268.
Whitaker ,T, Horwitz ,w, Albert,R., neshim , S, 1996.Variability associated whit analytical methods used to measure aflatoxin in agricultural commodities .J .Assoc. Off. Anal. chem , 79:476- 485.
Volume 3, Issue 3 - Serial Number 3
September and October 2015Pages 241-244
- Receive Date 05 September 2015
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