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The Vitamin D Receptor (VDR) Gene Polymorphisms Fok1 In Obese Pakistanis – a Preliminary Report
Document Type : Original Article
Authors
1 Lecturer, Department of Biochemistry Ziauddin University Clifton, Karachi, Pakistan
2 Professor, Department of Biochemistry Ziauddin University Clifton, Karachi, Pakistan
Abstract
Objective: The role of vitamin D has soared to a pinnacle in recent years with functions affecting 229 human genes linked to cardiovascular, autoimmune, humoral, pulmonary and neurological diseases. Relationship between obesity and vitamin D has not as yet been fully established. The objective of the study was to determine the possible relationship between Vitamin D receptor (VDR) gene polymorphisms and the risk of obesity. Methods: Total 100 subjects (50 obese and 50 normal) were selected. Oral rinse samples were collected (between 18-45 years of age) after an informed consent. DNA was extracted and PCR was performed using VDR-Fok1 primers. A 256bp amplified products was visualized by Gel Doc Hero Lab software (Germany). Results: The results of VDR-Fok1 gene polymorphism showed that out of 50 obese subjects 40%were normal (FF=20), 54%were Heterozygous (Ff = 27) and 6% were mutated (ff= 3). Out of 50 non-obese subjects 84% were normal (FF= 42), 16% were Heterozygous (Ff= 8) and none were mutated (ff= 0%). The statistical analysis results between the ff genotype and obesity were not significant. The ff genotype of VDR may be associated with obesity. More studies with larger sample size are required to find a stronger correlation.
Keywords
Subjects
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Grundberg E, Brandstrom H, Ribom EL, Ljunggren O, Mallmin H, Kindmark A . Genetic variation in the human vitamin D receptor is associated with muscle strength, fat mass and body weight in Swedish women. Eur J Endocrinol 2004; 150: 323
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Pharmacologica Sinica (2009) 30: 1634–1642; doi:10.1038/aps.2009.169
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Tang ZH, Xiao P, Lei SF, Deng FY, Zhao LJ, et al. A bivariate whole-genome linkage scan suggests several shared genomic regions for obesity and osteoporosis. J Clin Endocrinol Metab 2007; 92: 2751–7.
Uitterlinden AG, Fang Y, Bergink AP, van Meurs JB, van Leeuwen HP, Pols HA. The role of vitamin D receptor gene polymorphisms in bone biology. Mol Cell Endocrinol 2002;197: 15–21.
Vaidya A, Forman JP, Williams JS. Vitamin D and the vascular sensitivity to angiotensin II in obese Caucasians with hypertension. J Hum Hypertens. 2011
Nov;25(11):672-8. doi: 10.1038/jhh.2010.110. Epub 2010 Dec 2.
Vuolo L, Di Somma C, Faggiano A, Colao A. Vitamin D and cancer. Front Endocrinol (Lausanne). 2012 Apr 23;3:58. doi: 10.3389/fendo.2012.00058. eCollection 2012
Yiannis Vasilopoulos, Theologia Sarafidou, Kalliopi Kotsa, Maria Papadimitriou, Yiannis Goutzelas, Costas Stamatis, Vasilis Bagiatis, Xanthi Tsekmekidou, John G. Yovos, Zissis Mamuris VDR TaqI is associated with obesity in the
Greek population Gene Volume 512, Issue 2, 10 January 2013, Pages 237–239
Zhang K, Song L. Association between Vitamin D Receptor Gene Polymorphisms and Breast Cancer Risk: A Meta-Analysis of 39 Studies. PLoS One. 2014 Apr 25;9(4):e96125. doi: 10.1371/journal.pone.0096125.
eCollection 2014
Zhao LJ, Liu YJ, Liu PY, Hamilton J, Recker RR, et al. Relationship of obesity with osteoporosis. J Clin Endocrinol Metab. 2007;92:1640–1646.
Zhao XZ, Yang BH, Yu GH, Liu SZ, Yuan ZY. Polymorphisms in the vitamin D receptor (VDR) genes and skin cancer risk in European population: a metaanalysis. Arch Dermatol Res. 2014 Apr 28.
Zhang ZL, He JW, Qin YJ, Hu YQ, Li M, Zhang H, et al. Association between myostatin gene polymorphisms and peak BMD variation in Chinese nuclear families. Osteoporosis Int 2008; 19: 39–47
Bhatt SP, Misra A, Sharma M, Guleria R, Pandey RM, Luthra K, Vikram NK.Vitamin D Insufficiency Is Associated with Abdominal Obesity in Urban Asian Indians Without Diabetes in North India. Diabetes Technol Ther. 2014 Feb 14.
David Crawford Population strategies to prevent obesity. Only few studies attempted so far and with limited success BMJ. Oct 5, 2002; 325(7367): 728–729.
Dace A, Martin-el Yazidi C, Bonne J, Planells R , Torresani J. Calcitriol is a positive effector of adipose differentiation in the OB 17 cell line: relationship with the adipogenic action of triiodothyronine. Biochem Biophys Res Commun
1997; 232: 771–6.
Farooqi IS, Matarese G, Lord GM, Keogh JM, Lawrence E, Agwu C, Sanna V, Jebb SA, Perna F, Fontana S, Lechler RI, DePaoli AM, O’Rahilly S. Beneficial effects of leptin on obesity, T cell hyporesponsiveness, and
neuroendocrine/metabolic dysfunction of human congenital leptin deficiency. J Clin Invest. 2002;110:1093–1103.
Grundberg E, Brandstrom H, Ribom EL, Ljunggren O, Mallmin H, Kindmark A . Genetic variation in the human vitamin D receptor is associated with muscle strength, fat mass and body weight in Swedish women. Eur J Endocrinol 2004; 150: 323
Gitahi Theuri and Festus Kiplamai. Association between vitamin D levels and central adiposity in an eastern Africa outpatient clinical population DermatoEndocrinology 5:1, 218–221; January/February/March 2013;
Harris SS, Eccleshall TR, Gross C, et al The vitamin D receptors start codon polymorphism (Fok-1) and bone mineral density in premenopausal American Black and White women. J Bone Miner Res 1997: 12, 1043-8.
Jie-mei Gu, Wen-jin Xiao, Jin-wei He, Hao Zhang, Weiwei Hu, Yun-qiu Hu, Miao Li, etal Association between VDR and ESR1 gene polymorphisms with bone and obesity phenotypes in Chinese male nuclear families Acta
Pharmacologica Sinica (2009) 30: 1634–1642; doi:10.1038/aps.2009.169
Lucky MH, Baig S. Isolation of DNA from Oral rinse from HPV positive patients. Journal of the College of Physician and Surgeons Pakistan 2013, Vol. 23 (7):455-458
Morán-Auth Y, Penna-Martinez M, Shoghi F, RamosLopez E, Badenhoop K.Vitamin D status and gene transcription in immune cells. J Steroid Biochem Mol Biol. 2013 Jul;136:83-5. Doi
Tang ZH, Xiao P, Lei SF, Deng FY, Zhao LJ, et al. A bivariate whole-genome linkage scan suggests several shared genomic regions for obesity and osteoporosis. J Clin Endocrinol Metab 2007; 92: 2751–7.
Uitterlinden AG, Fang Y, Bergink AP, van Meurs JB, van Leeuwen HP, Pols HA. The role of vitamin D receptor gene polymorphisms in bone biology. Mol Cell Endocrinol 2002;197: 15–21.
Vaidya A, Forman JP, Williams JS. Vitamin D and the vascular sensitivity to angiotensin II in obese Caucasians with hypertension. J Hum Hypertens. 2011
Nov;25(11):672-8. doi: 10.1038/jhh.2010.110. Epub 2010 Dec 2.
Vuolo L, Di Somma C, Faggiano A, Colao A. Vitamin D and cancer. Front Endocrinol (Lausanne). 2012 Apr 23;3:58. doi: 10.3389/fendo.2012.00058. eCollection 2012
Yiannis Vasilopoulos, Theologia Sarafidou, Kalliopi Kotsa, Maria Papadimitriou, Yiannis Goutzelas, Costas Stamatis, Vasilis Bagiatis, Xanthi Tsekmekidou, John G. Yovos, Zissis Mamuris VDR TaqI is associated with obesity in the
Greek population Gene Volume 512, Issue 2, 10 January 2013, Pages 237–239
Zhang K, Song L. Association between Vitamin D Receptor Gene Polymorphisms and Breast Cancer Risk: A Meta-Analysis of 39 Studies. PLoS One. 2014 Apr 25;9(4):e96125. doi: 10.1371/journal.pone.0096125.
eCollection 2014
Zhao LJ, Liu YJ, Liu PY, Hamilton J, Recker RR, et al. Relationship of obesity with osteoporosis. J Clin Endocrinol Metab. 2007;92:1640–1646.
Zhao XZ, Yang BH, Yu GH, Liu SZ, Yuan ZY. Polymorphisms in the vitamin D receptor (VDR) genes and skin cancer risk in European population: a metaanalysis. Arch Dermatol Res. 2014 Apr 28.
Zhang ZL, He JW, Qin YJ, Hu YQ, Li M, Zhang H, et al. Association between myostatin gene polymorphisms and peak BMD variation in Chinese nuclear families. Osteoporosis Int 2008; 19: 39–47
Volume 3, Issue 3 - Serial Number 3
September and October 2015Pages 257-262
- Receive Date 05 September 2015
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