Document Type : Original Article


1 M.Sc of Agricultural and Natural Resources Research and Education Center, Ilam, Iran.

2 Department of Agriculture, Payame Noor University of Iran

3 Ph.D. Student of Plant Breeding Bu-Ali Sina, Hamedan, Iran



The present study was laid out in order toevaluate the effect of salinity on some biochemical characterize of grapevine c.vYaghouti. The experiment was done at Agricultural and Natural Resources Research Center, Ilam, Iran at 2014. The experiment was a completely block design (CRD) with four replications. Saline factor was NaCl salinity in seven levels (Zero, 50, 100, 150, 200 and 250 mM). Grape scions were planted in beds containing equal proportions of sand, perlite and vermiculite in 20-L vases. The vases were nourished by half Hoagland nutrient solution for first three weeks after plantation. In order to create the desired salinity levels, another half of the Hoagland nutrient solution was salinized with 0, 50, 100, 150, 200 and 250 mMNaCl. During growth stages, measurement was done on physiological and biochemical parameters such as soluble sugar, proline, chlorophyll, catalase, peroxidase and ascorbat peroxidase enzymes. The results showed that maximum chlorophyll a and b were obtained for control treatment and decreased gradually with increasing of saline level. The proline and soluble content as osmolits increased gradually by increasing of saline levels. However, CAT, APX and POX activity increased by salinity levels until 150mM, 150mM and 250mM saline treatment respectively. The results of the present study reviled that salt stress decreased some biochemical traits and decreased some damages by increasing of osmolits content and antioxidant enzymes activity.


Bates, I.S., Waldern, R.P., Teare, I.D., 1973. Rapid determination of free proline for water stress studies. Plant. Soil., 39, 905-207.
BERGER, M.M., 2005. Can oxidative damage be treated nutritionally. Clin. Nutr., 24, 172-183.
Blokhina, O., Virolainen, E., Fagerstedt, K.V., 2003. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann. Botan., 91, 179–194.
Cavagnaro, J.B., Ponce, M.T., Guzman, J., Cirrincione, M.A., 2006. Argentinean cultivars ofVitisviniferagrow better than European ones when cultured in vitro under salinity. Biocell., 30(1), 1-7.
Dettweiler, E., Eibach, R., 2003. The two Vitis databases as tools for germplasm management Vitis international variety catalogue and European Vitis database. Acta. Horticulture., 603, 505-509.
Kishor, P.B.K., Sangam, S., Amrutha, R.N., Laxmi, P.S., Naidu, K.R., Rao, K.S., 2005. Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implications in plant growth and abiotic stress tolerance. Curr. Sci., 88, 424-38.
Koca, M., Bor, M., Ozdemir, F., Turkan, I., 2007. The effect of salt stress on lipid peroxidation, antioxidative enzymes and proline content of sesame cultivars. Environ. Exp. Bot., 60, 344–351.
Nakano, Y., Asada, K., 1981. Hydrogen peroxide scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant. Cell. Physiol., 22, 867–880.
Noctor, G., Foyer, C.H., 1998. Ascorbate and glutathione: keeping active oxygen under control. Ann. Rev. Plant. Physiol. Plant. Molecul. Biol., 49, 249–279.
Philippe, M., Koraljka, R.K., Karlheinz, B., 2007. A simple method to measure effective catalase activities: optimization, validation, and application in green coffee. Analytic. Biochem., 360, 207–215.
Sadasivam, N., Manickam, A., 1996. Biochemical Methods for Agricultural Sciences, New Age International (P) Ltd., New Delhi.
Schwarz, M., 1995. Soilless culture management. Adv. Ser. Agr. Sci., 24, 197.
Serraj, R., Sinclair, T.R., 2002. Osmolyte accumulation: can it really help increase crop yield under drought conditions? Plant. Cell. Environ., 25, 333-341.
Shani, U., Ben-Gal, Al., 2005. Long-term response of grapevines to salinity: Osmotic effects and ion toxicity. Am. J. Enol. Vitic., 56, 148-154.
Voetberg, G.S., Sharp, R.E., 1991. Growth of the maize primary root in low water potentials. III. Roles of increased praline depositions in osmotic adjustment. Plant. Physiol., 96, 125-30.
Youssef, A.M., Al-Fredan, M.A., 2008. Community composition of major vegetations in the coastal area of Al-Uqair, Saudi Arabia in response to ecological variations. J. Biol. Sci., 8, 713-721.