International Journal of Advanced Biological and Biomedical Research

MSRT,ISC
37
h-index

Investigation on the using of linco-spectin solution for in ovo administration in chicken embryo

Document Type : Original Article

Authors

Hadi Tavakkoli 1
Amin Derakhshanfar 2
Sajedeh Salandari 3

1 Department of Avian Medicine, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

2 Department of Pathobiology, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

3 School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

Abstract
Nowadays antibiotics are used on a large scale in veterinary and human medicine to cure or prevent diseases. Some antibiotics injected into hatching eggs to eliminate pathogens and prevention of egg transmission of disease. Adverse effects of drugs have always been a major concern. There is scantly information available about the safety and pathological alterations of lincosamide-aminocyclitol compounds in embryo. The objective of this study was to investigate using of various dosages of Linco-spectin solution for in ovo administration in chicken embryo. Fertile chicken eggs were divided into six equal treatment groups as follows: group 1: uninjected group. Group 2: needle-injected group; the needle was inserted into the yolk sac without any injection. Group 3: phosphate buffered saline-injected group; whose individuals were injected with phosphate buffered saline. Groups 4, 5 and 6 whose individuals were injected with linco-spectin solution at a dosage of 10 mg lincomycin and 20 mg spectinomycin or 15 mg lincomycin and 30 mg spectinomycin or 20 mg lincomycin and 40 mg spectinomycin per Kg egg-weight, respectively. Embryos were re-incubated post-treatment and allowed to develop until day 18 after which; they were examined for macroscopic and microscopic lesions. Results showed that embryos were normal in all treatment groups. Microscopically, no lesions were also diagnosed in tissues. Based on macroscopic and microscopic findings, it is concluded that linco-spectin at above-mentioned concentration is not toxic for the chicken embryo. So, linco-spectin egg-injection can be used toeliminate pathogens and prevention of egg transmission of the diseasewithout any adverse effect.

Keywords

Chicken
egg
embryo
Linco-spectin
pathology
Agunos A, Léger D, Carson C (2012). Review of antimicrobial therapy of selected bacterial diseases in broiler chickens in Canada. The Canadian Veterinary Journal 53(12): 1289.
Ahrens FA, Martin RJ (2013). Antimicrobial drugs. Handbook of Veterinary Pharmacology 347.
Dubb A (2012). Antibiotic Update. South African Family Practice 4(5).
Garner DL, Evans KM, Seidel GE (2013). Sex-Sorting Sperm Using Flow Cytometry/Cell Sorting. Spermatogenesis, Springer: 279-295.
Gharaibeh S, Al-Rashdan M (2011). Change in antimicrobial susceptibility ofMycoplasma gallisepticumfield isolates. Veterinary microbiology 150(3): 379-383.
Ghazikhanian GY, Yamamoto R, McCapes R, Dungan WM, Larsen C, Ortmayer H (1980). Antibiotic Egg Injection to Eliminate Disease II. Elimination of Mycoplasma meleagridis from a Strain of Turkeys. Avian Diseases 48-56.
Hamburger V (1942). A manual of experimental embryology, University of Chicago Press Chicago.
Jacobsen ID, Große K, Hube B (2012). Embryonated Chicken Eggs as Alternative Infection Model for Pathogenic Fungi. Host-Fungus Interactions, Springer: 487-496.
Kleven S (2008). Control of avian mycoplasma infections in commercial poultry. Avian Diseases 52(3): 367-374.
McCapes R, Yamamoto R, Ghazikhanian G, Dungan W, Ortmayer H (1977). Antibiotic Egg Injection to Eliminate Disease I. Effect of Injection Methods on Turkey Hatchability and Mycoplasma meleagridis Infection. Avian Diseases 57-68.
Nascimento ER, Pereira V, Nascimento M, Barreto M (2005). Avian mycoplasmosis update. Revista Brasileira de Ciência Avícola 7(1): 1-9.
Persoons D, Dewulf J, Smet A, Herman L, Heyndrickx M, Martel A, Catry B, Butaye P, Haesebrouck F (2012). Antimicrobial use in Belgian broiler production. Preventive Veterinary Medicine 105(4): 320-325.
Rasschaert G, Michiels J, Arijs D, Wildemauwe C, De Smet S, Heyndrickx M (2012). Effect of farm type on within-herd Salmonella prevalence, serovar distribution, and antimicrobial resistance. Journal of Food Protection 75(5): 859-866.
Sheeks OB, Sheeks RL (1992). Egg injection method, apparatus and carrier solution for improving hatchability and disease control, Google Patents.
Singroha R, Srivastava S, Chhikara P (2012). Effect of Gentamicin on kidney in developing chicks. Eur J Anat 16(2): 119-126.
Singroha R, Srivastava S, Chhikara P (2013). Effect of gentamicin on proximal convoluted tubules of kidney in developing chicks. Journal of the Anatomical Society of India 62(1): 17-22.
Swayne DE, Glison LR, McDonald LR, Nolan LK, Suarez DL, Nair V (2013). Disease of poultry, A John Wiley and Sons publication.
Sweetman SC, Pharm B, PharmS F, Eds. (2009). Martindale: The Complete Drug Reference. London, Pharmaceutical Press.
International Journal of Advanced Biological and Biomedical Research
Volume 2, Issue 1 - Serial Number 1
January and February 2014
Pages 110-116

  • Receive Date 24 July 2014

Article View 20,229
PDF Download 9,922

Home

A-Z Journals

SPC Publisher's Policies

Indexing and Abstracting

Editorial Board

Guide for Authors

EndNote file for authors

Submit Manuscript

Contact Us