Document Type : Original Article


1 Department of Pharmacology, Toxicology and Therapeutics, College of Medicine, University of Lagos, Lagos, Nigeria

2 Department of Biochemistry, Faculty of Life Sciences, Lagos State University, Lagos, Nigeria

3 Department of Internal Medicine, National Hospital Abuja, Abuja, Nigeria

4 Department of Pharmacy, Faculty of Pharmacy, Delta State University, Nigeria

5 Department of Pharmaceutical Microbiology, Delta State University, Abraka, Nigeria

6 Department of Biochemistry, Faculty of Life Sciences, University of Ilorin, Nigeria

7 Department of Biochemistry, School of Life Sciences, Federal University of Technology, Akure, Nigeria

8 Department of Science Education, Faculty of Education, Ekiti State University, Ado-Ekiti Ekiti, Nigeria

9 Department of Biomedical Technology, School of Basic Medical Science, The Federal University of Technology Akure, Nigeria

10 Department of Food Science and Technology, Federal University Oye Ekiti, Nigeria


Many seaweeds, most notably brown algae, are suitable for human consumption. These plants have various possible biological actions and significant phytochemical components. The ethylacetate and dichloromethane extracts of Sargassum flutans were examined for their phytochemical composition and in vitro antioxidant activities in our research. The antioxidant capacity was evaluated using the DPPH (2, 2-diphenyl-1-picrylhydrasyl) method and the reducing power. The DPPH radical scavenging activity of an antioxidant is a well-known indicator of its capacity to eliminate free radicals. Sargassum flutans ethylacetate extracts had demonstrated effective DPPH radical reducing capability. The extract's capacity to eliminate DPPH radicals increased with concentration. The typical antioxidant and positive control employed was ascorbic acid. Ethylacetate extracts from Sargassum flutans showed more reducing power than dichloromethane. The phenolic content was also determined using the Folin-Ciocalteau reagent to evaluate the extracts' impact on total antioxidant activity. The results show that the percentage of phenolic compounds of the Sargassum futans DCM extracts was higher than that of the ethylacetate extracts. Finally, it is also noteworthy to mention that, the DPPH scavenging, reducing power, and phenolic content in these extracts of maritime macroalgae were remarkably concentration dependent. 

Graphical Abstract

Phytochemical Profile and Antioxidant Properties of Extracts from Sargassum flutans


Main Subjects


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  1. Wijesekara I, Pangestuti R, Kim SK. Biological activities and potential health benefits of sulfated polysaccharides derived from marine algae, Carbohydrate polymers; 2011 Feb 11; 84(1):14-21. [Crossref], [Google Scholar], [Publisher]
  2. Menaa F, Wijesinghe U, Thiripuranathar G, Althobaiti NA, Albalawi AE, Khan BA, Menaa B. Marine algae-derived bioactive compounds: a new wave of nanodrugs?, Marine Drugs; 2021 Aug 26; 19(9):484. [Crossref], [Google Scholar], [Publisher]
  3. Remya RR, Rajasree SR. A study on bioactive compounds derived from brown seaweeds and their therapeutic applications towards various diseases, Research Journal of Pharmacy and Technology; 2016; 9(4):369-72. [Crossref], [Google Scholar], [Publisher]
  4. Uribe E, Vega-Gálvez A, García V, Pastén A, López J, Goñi G. Effect of different drying methods on phytochemical content and amino acid and fatty acid profiles of the green seaweed, Ulva spp, Journal of Applied Phycology; 2019 Jun 15; 31:1967-79. [Crossref], [Google Scholar], [Publisher]
  5. Katsanos CS, Kobayashi H, Sheffield-Moore M, Aarsland A, Wolfe RR. A high proportion of leucine is required for optimal stimulation of the rate of muscle protein synthesis by essential amino acids in the elderly, American Journal of Physiology-Endocrinology and Metabolism; 2006 Aug; 291(2):E381-7. [Crossref], [Google Scholar], [Publisher]
  6. Oberbauer AM, Larsen JA. Amino acids in dog nutrition and health, Amino Acids in Nutrition and Health: Amino Acids in the Nutrition of Companion, Zoo and Farm Animals; 2021:199-216. [Crossref], [Google Scholar], [Publisher]
  7. Johnson M, Kanimozhi SA, Malar TR, Shibila T, Freitas PR, Tintino SR, Menezes IR, da Costa JG, Coutinho HD. The antioxidative effects of bioactive products from Sargassum polycystum C. Agardh and Sargassum duplicatum J. Agardh against inflammation and other pathological issues, Complementary therapies in medicine; 2019 Oct 1; 46:19-23. [Crossref], [Google Scholar], [Publisher]
  8. Yende SR, Harle UN, Chaugule BB. Therapeutic potential and health benefits of Sargassum species, Pharmacognosy reviews; 2014 Jan; 8(15):1. [Crossref], [Google Scholar], [Publisher]
  9. Carvalho M, Ferreira PJ, Mendes VS, Silva R, Pereira JA, Jerónimo C, Silva BM. Human cancer cell antiproliferative and antioxidant activities of Juglans regia L, Food and chemical toxicology; 2010 Jan 1; 48(1):441-7. [Crossref], [Google Scholar], [Publisher]
  10. Liu RH. Potential synergy of phytochemicals in cancer prevention: mechanism of action, J. Nutr.; 2004 Dec 1; 134(12):3479S-85S. [Crossref], [Google Scholar], [Publisher]
  11. Leite AM, Mayo B, Rachid CT, Peixoto RS, Silva JT, Paschoalin VM, Delgado S. Assessment of the microbial diversity of Brazilian kefir grains by PCR-DGGE and pyrosequencing analysis, Food Microbiol.; 2012 Sep 1; 31(2):215-21. [Crossref], [Google Scholar], [Publisher]
  12. Kähkönen MP, Hopia AI, Heinonen M. Berry phenolics and their antioxidant activity, J. Agric. Food Chem.; 2001 Aug 20; 49(8):4076-82. [Crossref], [Google Scholar], [Publisher]
  13. Halliwell B, Zentella A, Gomez EO, Kershenobich D. Antioxidants and human disease: a general introduction, Nutr. Rev.; 1997; 55(1):S44. [Crossref], [Google Scholar], [Publisher]
  14. Mao QQ, Xu XY, Cao SY, Gan RY, Corke H, Beta T, Li HB. Bioactive compounds and bioactivities of ginger (Zingiber officinale Roscoe), Foods; 2019 May 30; 8(6):185. [Crossref], [Google Scholar], [Publisher]
  15. Van Buren JP, Robinson WB. Formation of complexes between protein and tannic acid, J. Agric. Food Chem.; 1969 Jul; 17(4):772-7. [Crossref], [Google Scholar], [Publisher]
  16. Obadoni BO, Ochuko PO. Phytochemical studies and comparative efficacy of the crude extracts of some haemostatic plants in Edo and Delta States of Nigeria, Glob. J. Pure Appl. Math.; 2002; 8(2):203-8. [Crossref], [Google Scholar], [Publisher]
  17. Solich P, Sedliakova V, Karlíček R. Spectrophotometric determination of cardiac glycosides by flow-injection analysis, Anal. Chim. Acta; 1992 Nov 20; 269(2):199-203. [Crossref], [Google Scholar], [Publisher]
  18. Hinneburg I, Dorman HD, Hiltunen R. Antioxidant activities of extracts from selected culinary herbs and spices, Food Chem.; 2006 Jul 1; 97(1):122-9. [Crossref], [Google Scholar], [Publisher]
  19. Gulcin İ. Antioxidants and antioxidant methods: An updated overview, Arch. Toxicol.; 2020 Mar; 94(3):651-715. [Crossref], [Google Scholar], [Publisher]
  20. Mustafa RA, Hamid AA, Mohamed S, Bakar FA. Total phenolic compounds, flavonoids, and radical scavenging activity of 21 selected tropical plants, J. Food Sci.; 2010 Jan; 75(1):C28-35. [Crossref], [Google Scholar], [Publisher]
  21. Bansal A, Priyadarsini C. Medicinal properties of phytochemicals and their production. [Crossref], [Google Scholar], [Publisher]
  22. Zhang J, Hu K, Di L, Wang P, Liu Z, Zhang J, Yue P, Song W, Zhang J, Chen T, Wang Z. Traditional herbal medicine and nanomedicine: Converging disciplines to improve therapeutic efficacy and human health, Adv. Drug Deliv. Rev.; 2021 Nov 1; 178:113964. [Crossref], [Google Scholar], [Publisher]
  23. Walker B. Interaction of glycoalkaloids with model membranes. University of Missouri-Saint Louis; 2009. Google Scholar], [Publisher]
  24. Makarewicz M, Drożdż I, Tarko T, Duda-Chodak A. The interactions between polyphenols and microorganisms, especially gut microbiota, Antioxidants; 2021 Jan 28; 10(2):188. [Crossref], [Google Scholar], [Publisher]
  25. Hassan A, Akmal Z, Khan N. The phytochemical screening and antioxidants potential of Schoenoplectus triqueter L. Palla, J. Chem.; 2020 Sep 30; 2020:1-8. [Crossref], [Google Scholar], [Publisher]
  26. Zandi K, Tajbakhsh S, Nabipour I, Rastian Z, Yousefi F, Sharafian S, Sartavi K. In vitro antitumor activity of Gracilaria corticata (a red alga) against Jurkat and molt-4 human cancer cell lines, AJB; 2010; 9(40):6787-90. [Google Scholar], [Publisher]
  27. Norra I, Aminah A, Suri R, Arif Zaidi J. Effect of drying temperature on the content of fucoxanthin, phenolic and antioxidant activity of Malaysian brown seaweed, Sargassum sp, JTAFS; 2017; 45(1):25-36. [Google Scholar], [Publisher]
  28. Dang TT, Bowyer MC, Van Altena IA, Scarlett CJ. Comparison of chemical profile and antioxidant properties of the brown algae, Int. J. Food Sci. Technol.; 2018 Jan; 53(1):174-81. [Crossref], [Google Scholar], [Publisher]
  29. Engwa GA. Free radicals and the role of plant phytochemicals as antioxidants against oxidative stress-related diseases. Phytochemicals: source of antioxidants and role in disease prevention, BoD–Books on Demand; 2018 Nov 7; 7:49-74. [Crossref], [Google Scholar], [Publisher]
  30. Kaur C, Kapoor HC. Antioxidants in fruits and vegetables–the millennium’s health, Int. J. Food Sci. Technol.; 2001 Oct 20; 36(7):703-25. [Crossref], [Google Scholar], [Publisher]
  31. Agbaje EO, Charles OO. Spondias mombin Linn. (Anacardiaceous) Essential Oil Ointment Enhances Healing of Excision Wounds in Rats. [Google Scholar], [Publisher]