Document Type : Original Article


1 Department of Biochemistry, Faculty of Life Science, Federal University of Technology Minna, Bosso LGA, Niger State, Nigeria

2 Department of Chemistry and Biochemistry, College of Health and Natural Science, The University of Oklahoma, Tulsa, Tulsa, United States of America


Hyperuricemia presents as a prevailing concern in routine clinical practice, affecting an estimated 8.9% to 24.4% of the general populace. Central to the emergence of hyperuricemia and its associated ailments is the enzyme xanthine oxidase (XO), which participates in the breakdown of purines into uric acid. This investigation is dedicated to appraising the ability of Jatropha tanjorensis methanolic extract to counteract hyperuricemia and inhibit XO in mice induced with pyrazinamide. The isolation of XO from cow milk was accomplished using ammonium sulfate precipitation techniques, followed by quantification of its activity via spectrophotometric measurements at a wavelength of 293 nm. Evaluation of uric acid levels was carried out through an enzyme colorimetric approach. The yield of Jatropha tanjorensis was found to be 9.2% (27.6 g). The XO inhibitory action of the extract was evident in the reduction in uric acid concentration from 8.73 μg/ml to 4.45 μg/ml after 1 hour. Assessment of toxicity showed that the extract, even at a high dose of 8000 mg/kg bw, had no observable impact on the animals’ behavior or physical appearance. The uric acid assay demonstrated that all three doses of Jatropha tanjorensis extract led to a noteworthy reduction in mean serum uric acid levels compared to the negative control group. The highest mean uric acid levels were noted in the negative control groups, measuring 5.36 ± 0.40 mg/dl and 2.71 ± 0.34 mg/dl, respectively. In contrast, the positive control group treated with febuxostat exhibited a mean uric acid level of 3.15 ± 0.27 mg/dl. Regarding weight changes, the normal control and positive control groups displayed average gains of 62.53 g and 35.94 g, respectively, while the negative control group did not exhibit substantial weight variation. Consequently, the Jatropha tanjorensis extract induced a notable reduction in serum uric acid levels, and this antihyperuricemic effect became more prominent as the extract concentration increased. This suggests the extract's potential value in managing hyperuricemia.

Graphical Abstract

Anti-hyperuricemic and Xanthine Oxidase Inhibitory Effects of Jatropha tanjorensis Methanolic Extract in Pyrazinamide-Induced Mice


Main Subjects



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  1. Feng Y, Fu M, Guan X, Wang C, Meng H, Zhou Y, He M, Guo H. Associations of exposure to perfluoroalkyl substances with serum uric acid change and hyperuricemia among Chinese women: Results from a longitudinal study, Chemosphere; 2022 Dec 1; 308:136438. [Crossref], [Google Scholar], [Publisher]
  2. Zhao C, Xiao J, Liu T, Shi H, Li Q, Ruan Z. Electrochemical sensor based on glass carbon electrode modified with graphene quantum dots (GQDs) for detection of uric acid, International Journal of Electrochemical Science; 2022 Sep 1; 17(9):22096. [Crossref], [Google Scholar], [Publisher]
  3. Wołyniec W, Szwarc A, Kasprowicz K, Zorena K, Jaskulak M, Renke M, Naczyk M, Ratkowski W. Impact of hydration with beverages containing free sugars or xylitol on metabolic and acute kidney injury markers after physical exercise, Frontiers in Physiology; 2022:2162. [Crossref], [Google Scholar], [Publisher]
  4. Maher L, Oghene JE, Reginato AM. Crystal-Induced Arthritis, Rheumatology for Primary Care Providers: A Clinical Casebook; 2022:147-210. [Crossref], [Google Scholar], [Publisher]
  5. Webster JS, Kaplow R. Tumor lysis syndrome: implications for oncology nursing practice. InSeminars in Oncology Nursing 2021 Apr 1; 37(2):151136. [Crossref], [Google Scholar], [Publisher]
  6. Ramos GK, Goldfarb DS. Update on uric acid and the kidney, Current Rheumatology Reports; 2022 May; 24(5):132-8. [Crossref], [Google Scholar], [Publisher]
  7. Tao H, Mo Y, Liu W, Wang H. A review on gout: Looking back and looking ahead, International Immunopharmacology; 2023 Apr 1; 117:109977. [Crossref], [Google Scholar], [Publisher]
  8. Bortolotti M, Polito L, Battelli MG, Bolognesi A. Xanthine oxidoreductase: One enzyme for multiple physiological tasks, Redox Biology; 2021 May 1; 41:101882. [Crossref], [Google Scholar], [Publisher]
  9. Stallings TL, McCray N, Lau J, Salinas A, Ramírez-Rubio O, López-Pilarte D, Amador Velazquez J, Riefkohl Lisci A, Weiner DE, Friedman DJ, Brooks DR. Herbal remedies and hyperuricemia in a Central American population with elevated chronic kidney disease of unknown origin, InISEE Conference Abstracts; 2020 Aug 27; 2020(1). [Crossref], [Google Scholar], [Publisher]
  10. Bupparenoo P, Pakchotanon R, Narongroeknawin P, Asavatanabodee P, Chaiamnuay S. Effect of curcumin on serum urate in asymptomatic hyperuricemia: A randomized placebo-controlled trial, Journal of Dietary Supplements; 2021 May 4; 18(3):248-60. [Crossref], [Google Scholar], [Publisher]
  11. Ahmad M, Sultana S, Asif HM, Ur Rehman J, Anhtar N, Iqbal Z, Owais A, Rehman A, Abbas MK. Hyperuricemia and role of medicinal plants: mini, World Journal of Pharmacy and Pharmaceutical Sciences; 2018 Jan 22; 7(4):40-53. [Crossref], [Google Scholar], [Publisher]
  12. Abu Bakar FI, Abu Bakar MF, Rahmat A, Abdullah N, Sabran SF, Endrini S. Anti-gout potential of Malaysian medicinal plants, Frontiers in pharmacology; 2018 Mar 23;9:261. [Crossref], [Google Scholar], [Publisher]
  13. Busari MB, Egwim EC, Babayi HM, Ibrahim YO, Okorie IK. Comparative Phytochemical Constituents and Antioxidant Activities of Hot and Cold Methanol Extract of Taminalia catappa Leaves, 9th Jatropha International Conference and Exhibition 2019 Jatropha 2019. [Crossref], [Google Scholar], [Publisher]
  14. JI N, Uwah AF, Effiong BO, Bassey UE, Umanah BM, Chukwudike CP. Ethanol leaf extract of Jatropha tanjorensis ameliorates hepatorenal toxicity of Plasmodium berghi-berghi infected mice treated with Hippocratea africana root bark extract, The Journal of Phytopharmacology; 2020; 9(5):374 [Crossref], [Google Scholar], [Publisher]
  15. Odogwu BA, Ukomadu J, Igwe I. Morphological, phytochemical and molecular characterization of five common Jatropha species in the Niger Delta Region of Nigeria, Journal of Applied Sciences and Environmental Management; 2021 Dec 28; 25(9):1637-44. [Crossref], [Google Scholar], [Publisher]
  16. Cavalcante NB, da Conceição Santos AD, da Silva Almeida JR. The genus Jatropha (Euphorbiaceae): A review on secondary chemical metabolites and biological aspects, Chemico-biological interactions; 2020 Feb 25; 318:108976. [Crossref], [Google Scholar], [Publisher]
  17. Chigozie OO, Uzoma NO, Ikechukwu UR, Ikechukwu ES. Nutritional composition of Jatropha tanjorensis leaves and effects of its aqueous extract on carbon tetrachloride induced oxidative stress in male Wistar albino rats, Biomedical Research; 2018; 29(19):3569-76. [Crossref], [Google Scholar], [Publisher]
  18. Ajah O, Onyedikachi UB, Alaebo PO, Odo CE, Godwin OK, Omodamiro OD. Methanol leaf extract of Jatropha tanjorensis Ellis and Saroja possess phytoconstituents with free radical scavenging activity, Fudma Journal Of Sciences; 2021 Nov 3; 5(3):286-93. [Crossref], [Google Scholar], [Publisher]
  19. Natsir H, Arif AR, Wahab AW, Budi P, Arfah RA, Arwansyah A, Fudholi A, Suriani NL, Himawan A. Inhibitory effects of Moringa oleifera leaves extract on xanthine oxidase activity from bovine milk, Pharmacia; 2022 Apr 14; 69(2):363-75. [Crossref], [Google Scholar], [Publisher]
  20. Waheed SA, Chioma CF, Ibraheem YO, Sadiq MG. Evaluation of inhibitory activity of aqueous, methanol and ethyl acetate extracts of Citrus aurantifolia leaf on crude xanthine oxidase isolated from cow milk, International Journal of Applied Chemical and Biological Sciences; 2022 Mar 14; 3(2):1-8. [Crossref], [Google Scholar], [Publisher]
  21. Latif A, Ashiq K, Ashiq S, Ali E, Anwer I, Qamar S. Phytochemical analysis and in vitro Investigation of Anti-Inflammatory and xanthine Oxidase Inhibition Potential of Rroot Extracts of Bryophyllum Pinnatum, JAPS: Journal of Animal & Plant Sciences; 2020 Feb 1; 30(1). [Crossref], [Google Scholar], [Publisher]
  22. Fachriyah E, Ghifari MA, Anam K. Isolation, Identification, and Xanthine oxidase inhibition activity of alkaloid compound from Peperomia pellucida, InIOP Conference Series: Materials Science and Engineering; 2018 Apr 1; 349:012017. [Crossref], [Google Scholar], [Publisher]
  23. Hafeez A, Rehan AM, Hakim Z, Munir A, Khan RN, Khokhar A. Nigella sativa Seeds Protective Ability in Pyrazinamide Induced Hyperuricemia in Mice, InProceedings; 2022 Feb 1;36(1):44-48. [Crossref], [Google Scholar], [Publisher]
  24. Madaki FM, Adio SW, Busari MB, Kabiru AY, MANN A, Ogbadoyi EO. Antioxidant and Anti-trypanosomal Activities of the Allium sativum (Garlic) Bulb Aqueous Extract on Trypanosoma Congolense Infected Mice, Iranian Journal of Toxicology; 2022; 16(3):153-162. [Crossref], [Google Scholar], [Publisher]
  25. Unegbu CC, Ajah O, Nnorom CM, Onwusonye JC, Duru CA, Joel OM. Antidiabetes, antidyslipidemia, hemoprotective, nehproprotective and hepatoprotective effect of ethanol extract of Jatropha tanjorensis leaf against alloxan induced diabetes in rats, Journal of Medicinal Herbs; 2022 Apr 21; 13(1):57-64. [Crossref], [Google Scholar], [Publisher]
  26. Browne D, McGuinness B, Woodside JV, McKay GJ. Vitamin E and Alzheimer’s disease: what do we know so far?, Clinical interventions in aging; 2019 Jul 18:1303-17. [Crossref], [Google Scholar], [Publisher]
  27. Omotayo AO, Aremu AO. Underutilized African indigenous fruit trees and food–nutrition security: Opportunities, challenges, and prospects, Food and Energy Security; 2020 Aug; 9(3):e220. [Crossref], [Google Scholar], [Publisher]
  28. Ozioma EO, Chinwe OA. Herbal medicines in African traditional medicine, Herbal medicine; 2019 Jan 30; 10:191-214. [Crossref], [Google Scholar], [Publisher]
  29. Pius, O. G., & Uredo-ojo, F. B. (2022). Antimicrobial effect of Jatropha tanjorensis leaves on multi-antibiotic resistant bacteria isolated from poultry droppings. World Journals of Advanced Research and Reviews, 13(01): 775-780. [Crossref], [Google Scholar], [Publisher]
  30. Chibuogwu CC, Njoku UO, Nwodo FO, Ozougwu EV, Nweze NV. Toxicity assessment of the methanol extract of Jatropha tanjorensis (Euphorbiaceae) leaves, Future Journal of Pharmaceutical Sciences; 2021 Dec; 7:1-8. [Crossref], [Google Scholar], [Publisher]
  31. Pichholiya M, Yadav AK, Luhadia SK, Tahashildar J, Aseri ML. A comparative study of efficacy and safety of febuxostat and allopurinol in pyrazinamide-induced hyperuricemic tubercular patients, Indian Journal of Pharmacology; 2016 Sep; 48(5):522. [Crossref], [Google Scholar], [Publisher]
  32. Amaechi D, Yisa BN, Ekpe IP, Nwawuba PI, Rabbi A. Phytochemical Screening, Anti-obesity and Hepatoprotective Activities of Ethanol Leaf Extract of Jatropha tanjorensis in Wistar Rats, Asian Journal of Applied Chemistry Research; 2022 Dec 29; 12(4):20-6. [Crossref], [Google Scholar], [Publisher]
  33. Bredemeier M, Lopes LM, Eisenreich MA, Hickmann S, Bongiorno GK, d’Avila R, Morsch AL, da Silva Stein F, Campos GG. Xanthine oxidase inhibitors for prevention of cardiovascular events: a systematic review and meta-analysis of randomized controlled trials, BMC cardiovascular disorders; 2018 Dec; 18(1):1-1. [Crossref], [Google Scholar], [Publisher]
  34. Minh TN, Andriana Y, Minh BQ, Trung NQ, de Guzman-Gelani C. Investigation of xanthine oxidase inhibitors in bioactive components of Jatropha podagrica stem bark, Journal of Pharmaceutical Sciences; 2021; 45(4):4527-4530. [Crossref], [Google Scholar], [Publisher]
  35. Minh TN, Minh BQ, Duc TH, Thinh PV, Anh LV, Dat NT, Nhan LV, Trung NQ. Potential use of Moringa oleifera twigs extracts as an anti-hyperuricemic and anti-microbial source, Processes; 2022 Mar 14; 10(3):563. [Crossref], [Google Scholar], [Publisher]