Document Type : Original Article


1 Department of Pharmacology, Toxicology and Therapeutics, College of Medicine, University of Lagos, 101014, Nigeria

2 Department of Environmental Science, School of Environment, Northeast Normal University, 130024, China

3 Department of Plant Breeding, Crop Improvement Unit, Cocoa Research Institute of Nigeria (CRIN), Nigeria

4 Department of Biochemistry, Faculty of Life Sciences, Lagos State University, Lagos, 102101, Nigeria

5 Department of Internal Medicine, National Hospital Abuja, Abuja, 900211, Nigeria

6 Department of Horticulture, Faculty of Agriculture and Natural Resources, Kwame Nkrumah University of Science and Technology, Ghana


The objective of the current study was to assess the ethanolic and methanolic extracts of Carica papaya's hypoglycemic effects. Yeast glucose uptake, muscle glucose uptake, and glucose adsorption capacity were used to measure the extracts' in vitro hypoglycemic effects. The antioxidant capacity of the extracts was assessed by investigating how they affect lipid peroxidation brought on by iron (II) sulphate and sodium nitroprusside. The findings showed that glucose was absorbed by both the ethanolic and methanolic extracts of Carica papaya, and that this adsorption significantly increased as the concentration of glucose rose. There were no variations in their adsorption capabilities that were statistically significant (p=0.05). The yeast cells were also stimulated to take up glucose by the plant extracts, and this stimulation was influenced by the sample and glucose content. In the study's muscle glucose uptake, the ethanolic extract of Carica papaya leaves showed substantially greater (p=0.05) performance than the methanolic of the same leaves with increasing concentration. The study's findings showed that the plant's methanolic extract was substantially more potent than its ethanolic (p=0.05). In addition, the methanolic extract considerably inhibited the generation of MDA (malondialdehyde) in the liver and brain homogenates more than the ethanolic extract did. Both plant extracts also exhibit dose-dependent inhibition of the various pro-oxidant agents (Iron (II) Sulphate and sodium nitroprusside) caused fatty acid oxidation tissues present in the brain and liver.

Graphical Abstract

Hypoglycemic and Antioxidants Activities of Carica Papaya Leaves


Main Subjects



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  1. Pandey S, Chmelir T, Chottova Dvorakova M. Animal Models in Diabetic Research—History, Presence, and Future Perspectives, Biomedicines; 2023 Oct 20; 11(10):2852. [Crossref], [Google Scholar], [Publisher]
  2. Vieira R, Souto SB, Sánchez-López E, López Machado A, Severino P, Jose S, Santini A, Fortuna A, García ML, Silva AM, Souto EB. Sugar-lowering drugs for type 2 diabetes mellitus and metabolic syndrome—Review of classical and new compounds: Part-I, Pharmaceuticals; 2019 Oct 10; 12(4):152. [Crossref], [Google Scholar], [Publisher]
  3. Elmi GR, Anum K, Saleem K, Fareed R, Noreen S, Wei H, Chen Y, Chakraborty A, Rehman MU, Liyuan S, Abbas M. Evaluation of clinical trials of ethnomedicine used for the treatment of diabetes: A systematic review, Frontiers in Pharmacology; 2023 Apr 7; 14:822. [Crossref], [Google Scholar], [Publisher]
  4. Mohamed AI, Beseni BK, Msomi NZ, Salau VF, Erukainure OL, Aljoundi A, Islam MS. The antioxidant and antidiabetic potentials of polyphenolic-rich extracts of Cyperus rotundus (Linn.), Journal of Biomolecular Structure and Dynamics; 2022 Dec 12; 40(22):12075-87. [Crossref], [Google Scholar], [Publisher]
  5. Airaodion AI, Ogbuagu EO, Ekenjoku JA, Ogbuagu U, Okoroukwu VN. Antidiabetic effect of ethanolic extract of Carica papaya leaves in alloxan-induced diabetic rats, American Journal of Biomedical Science & Research; 2019 Sep 26; 5(3):227-34. [Crossref], [Google Scholar], [Publisher]
  6. Rethinam P, Krishnakumar V. Health Benefits of Coconut Water. InCoconut Water: A Promising Natural Health Drink-Distribution, Processing and Nutritional Benefits 2022 Oct 15:385. [Crossref], [Google Scholar], [Publisher]
  7. Solikhah TI, Setiawan B, Ismukada DR. Antidiabetic activity of papaya leaf extract (Carica Papaya L.) isolated with maceration method in alloxan-induces diabetic mice, Syst Rev Pharm; 2020 Sep 1; 11(9):774-8. [Crossref], [Google Scholar], [Publisher]
  8. Ajuru MG, Williams LF, Ajuru G. Qualitative and quantitative phytochemical screening of some plants used in ethnomedicine in the Niger Delta region of Nigeria, Journal of food and Nutrition Sciences; 2017 Oct 24; 5(5):198-205. [Crossref], [Google Scholar], [Publisher]
  9. Fernández-Lucas J, Castañeda D, Hormigo D. New trends for a classical enzyme: Papain, a biotechnological success story in the food industry, Trends in Food Science & Technology; 2017 Oct 1; 68:91-101. [Crossref], [Google Scholar], [Publisher]
  10. Shatri AM, Mumbengegwi DR. Ethnomedicinal use and phytochemical analysis of medicinal plants used to treat gastrointestinal conditions by Awambo people in Iikokola Village, Namibia, Scientific African; 2022 Nov 1; 18:e01428. [Crossref], [Google Scholar], [Publisher]
  11. Hakim RF. Effect of Carica papaya extract toward incised wound healing process in mice (Mus musculus) clinically and histologically, Evidence-Based Complementary and Alternative Medicine; 2019 Nov 19; 2019. [Crossref], [Google Scholar], [Publisher]
  12. Callixte C, Baptiste NJ, Arwati H. Phytochemical screening and antimicrobial activities of methanolic and aqueous leaf extracts of Carica papaya grown in Rwanda, Molecular and Cellular Biomedical Sciences; 2020 Mar 1; 4(1):39-44. [Crossref], [Google Scholar], [Publisher]
  13. Schultz F, Anywar G, Wack B, Quave CL, Garbe LA. Ethnobotanical study of selected medicinal plants traditionally used in the rural Greater Mpigi region of Uganda, Journal of Ethnopharmacology; 2020 Jun 28; 256:112742. [Crossref], [Google Scholar], [Publisher]
  14. Ali M, Salma U, Khan IA, Ahmad T, Bashir K, Khan T, Mahnashi MH, Alhasaniah AH, Al Awadh AA, Almazni IA, Alshahrani MM. Evaluation of the Antiasthmatic Activity of Carissa opaca in Animal Models. BioMed Research International. 2022 Sep 6;2022. [Crossref], [Google Scholar], [Publisher]
  15. Montalva L, Zani A. Assessment of the nitrofen model of congenital diaphragmatic hernia and of the dysregulated factors involved in pulmonary hypoplasia. Pediatric surgery international. 2019 Jan 15;35:41-61 [Crossref], [Google Scholar], [Publisher]
  16. Memudu AE, Oluwole TJ. The contraceptive potential of Carica papaya seed on oestrus cycle, progesterone, and histomorphology of the Utero-ovarian tissue of adult wistar rats. JBRA Assisted Reproduction. 2021 Jan;25(1):34. [Crossref], [Google Scholar], [Publisher]
  17. Kyu HH, Abate D, Abate KH, Abay SM, Abbafati C, Abbasi N, Abbastabar H, Abd-Allah F, Abdela J, Abdelalim A, Abdollahpour I. Global, regional, and national disability-adjusted life-years (DALYs) for 359 diseases and injuries and healthy life expectancy (HALE) for 195 countries and territories, 1990–2017: a systematic analysis for the Global Burden of Disease Study 2017, The Lancet; 2018 Nov 10; 392(10159):1859-922. [Crossref], [Google Scholar], [Publisher]
  18. Agada R, Thagriki D, Lydia DE, Khusro A, Alkahtani J, Al Shaqha MM, Alwahibi MS, Elshikh MS. Antioxidant and anti-diabetic activities of bioactive fractions of Carica papaya seeds extract, Journal of King Saud University-Science; 2021 Mar 1; 33(2):101342. [Crossref], [Google Scholar], [Publisher]
  19. Olorundare OE, Adeneye AA, Akinsola AO, Sanni DA, Koketsu M, Mukhtar H. Clerodendrum volubile ethanol leaf extract: a potential antidote to doxorubicin-induced cardiotoxicity in rats, Journal of Toxicology; 2020 Jul 4; 2020. [Crossref], [Google Scholar], [Publisher]
  20. Akpan H, Omotoso O, Olapade A, Ogbonna E, Negedu M, Akande A, Adedeji A, Oladipupo F, Orisadiran P. Antioxidant properties of Carica papaya on cadmium toxicity on prefrontal-cortex of adult wistar rats, European Journal of Medicinal Plants; 2018 May 12; 23(3):1-9. [Crossref], [Google Scholar]
  21. Duggan EW, Carlson K, Umpierrez GE. Perioperative hyperglycemia management: an update, Anesthesiology; 2017 Mar 1; 126(3):547-60. [Crossref], [Google Scholar], [Publisher]
  22. Farooq S, Sehgal A. Antioxidant activity of different forms of green tea: Loose leaf, bagged and matcha, Current Research in Nutrition and Food Science Journal; 2018 Apr 20; 6(1):35-40. [Crossref], [Google Scholar], [Publisher]
  23. Yang R, Yang E, Shen L, Modlin RL, Shen H, Chen ZW. IL-12+ IL-18 cosignaling in human macrophages and lung epithelial cells activates cathelicidin and autophagy, inhibiting intracellular mycobacterial growth. The Journal of Immunology. 2018 Apr 1;200(7):2405-17. [Crossref], [Google Scholar], [Publisher]
  24. Aune D. Plant foods, antioxidant biomarkers, and the risk of cardiovascular disease, cancer, and mortality: a review of the evidence, Advances in Nutrition; 2019 Nov 1; 10(Supplement_4):S404-21. [Crossref], [Google Scholar], [Publisher]
  25. Onose G, Anghelescu A, Blendea D, Ciobanu V, Daia C, Firan FC, Oprea M, Spinu A, Popescu C, Ionescu A, Busnatu Ș. Cellular and Molecular Targets for Non-Invasive, Non-Pharmacological Therapeutic/Rehabilitative Interventions in Acute Ischemic Stroke. International Journal of Molecular Sciences. 2022 Jan 14;23(2):907. [Crossref], [Google Scholar], [Publisher]
  26. Rocha PC, Thompson JR, Leite FS, Garcia PC. The advertisement call of Bokermannohyla flavopicta Leite, Pezzuti & Garcia, 2012 (Anura: Hylidae) from the mountains of Chapada Diamantina, Bahia, Brazil. [Crossref], [Google Scholar], [Publisher]
  27. Rafiei F, Safrin M, Wokke ME, Lau H, Rahnev D. Transcranial magnetic stimulation alters multivoxel patterns in the absence of overall activity changes, Human Brain Mapping; 2021 Aug 15; 42(12):3804-20. [Crossref], [Google Scholar], [Publisher]
  28. Goff HD, Repin N, Fabek H, El Khoury D, Gidley MJ. Dietary fibre for glycaemia control: Towards a mechanistic understanding, Bioactive carbohydrates and dietary fibre; 2018 Apr 1; 14:39-53. [Crossref], [Google Scholar], [Publisher]
  29. Baliyan S, Mukherjee R, Priyadarshini A, Vibhuti A, Gupta A, Pandey RP, Chang CM. Determination of antioxidants by DPPH radical scavenging activity and quantitative phytochemical analysis of Ficus religiosa, Molecules; 2022 Feb 16; 27(4):1326. [Crossref], [Google Scholar], [Publisher]
  30. Shivanagoudra SR, Perera WH, Perez JL, Athrey G, Sun Y, Jayaprakasha GK, Patil BS. Cucurbitane-type compounds from Momordica charantia: Isolation, in vitro antidiabetic, anti-inflammatory activities and in silico modeling approaches, Bioorganic Chemistry; 2019 Jun 1; 87:31-42. [Crossref], [Google Scholar], [Publisher]
  31. Zheng H, Wu J, Huang H, Meng C, Li W, Wei T, Su Z. Metabolomics analysis of the protective effect of rubusoside on palmitic acid-induced lipotoxicity in INS-1 cells using UPLC-Q/TOF MS, Molecular omics; 2019; 15(3):222-32. [Crossref], [Google Scholar], [Publisher]
  32. Zhong Y, Luo R, Liu Q, Zhu J, Lei M, Liang X, Wang X, Peng X. Jujuboside A ameliorates high fat diet and streptozotocin induced diabetic nephropathy via suppressing oxidative stress, apoptosis, and enhancing autophagy, Food and Chemical Toxicology; 2022 Jan 1; 159:112697. [Crossref], [Google Scholar], [Publisher]
  33. Kuzulugil D, Papeix G, Luu J, Kerridge RK. Recent advances in diabetes treatments and their perioperative implications, Current opinion in anaesthesiology; 2019 Jun; 32(3):398. [Crossref], [Google Scholar], [Publisher]
  34. Hariono M, Julianus J, Djunarko I, Hidayat I, Adelya L, Indayani F, Auw Z, Namba G, Hariyono P. The future of Carica papaya Leaf extract as an herbal medicine product, Molecules; 2021 Nov 17; 26(22):6922. [Crossref], [Google Scholar], [Publisher]
  35. Heena D, Sunil T. Carica papaya: Potential implications in human health, Current Traditional Medicine; 2019 Dec 1; 5(4):321-36. [Crossref], [Google Scholar], [Publisher]