Document Type : Original Article
Authors
- Olumakinde Charles Omiyale 1
- Edu Zainab 2
- Ngozi Maryann Nebolisa 3
- Ayomide Balikis Asebebe 4
- Daniel Ebubechi Obasi 5
- Adeleye Adegboyega Edema 6
- Sulaimon Olajuwon Abdul 7
- Uchechukwu Divine Donatus 8
- Obinna Kenneth Didigwu 9
- Derrick Tochukwu Menankiti 8
- Faith Philip Edem 10
- Blessing Oluwatobi Ojo 11
1 Department of Pharmacology, Toxicology and Therapeutics, College of Medicine, University of Lagos, 101014, Nigeria
2 Department of Biochemistry, Faculty of Life Sciences, Lagos State University, Lagos, 102101, Nigeria
3 Department of Pharmacy, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria
4 Department of Biochemistry, Faculty of Basic Medical Science, Olabisi Onabanjo University, Nigeria
5 Department of Medicine and Surgery, Faculty of Clinical Sciences, University of Ibadan, Oyo State, Nigeria
6 Department of Biochemistry, Faculty of Basic Medical Sciences, University of Ibadan, Nigeria
7 Department of Pharmacy, Faculty of Pharmacy, Obafemi Awolowo College of Health Science, Olabisi Onabanjo University, Ogun State, Nigeria
8 Department of Chemical Engineering, Faculty of Engineering, Chukwuemeka Odumegwu Ojukwu University, Nigeria
9 epartment of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Nigeria Nsukka, 410001 Enugu State, Nigeria
10 Department of Chemistry, Faculty of Sciences, Akwa-Ibom State University, Nigeria
11 Department of Human Physiology, Basic Medical Sciences Delta State University, Nigeria
Abstract
Medicinal plants are the most easily accessible health resource for the community and frequently the people’s preferred option. Momordica charantia (bitter gourd) is a member of the Cucurbitaceous plant used as a vegetable and in traditional medicine. This study aimed to evaluate the effect of water extract of Momordica charantia leaves on male Balb/c mice with acute ulcerative colitis. 28 male mice were acclimatized and equally divided into four groups. The control group was given normal saline; the dextran sulphate sodium group (DSS) received 3% Dextran sulfate sodium in drinking water for seven days; the water extract of Momordica charantia + dextran sulphate sodium group (WEMC+DSS) received water extract of Momordica Charantia for seven days before dextran sulphate sodium (DSS) was administered; the water extract of Momordica charantia group (WEMC) received water extract of Momordica charantia (150mg/kg b.wt.) for 14 days. The mice were daily weighed and sacrificed on the 15th day of the experiment. Their colon and brain were collected for evaluation of disease activity indices (DAI) of colitis, and oxidative and inflammatory damage via biochemical and histological assessments. Results obtained showed no significant difference at (p<0.05) in DAI among the groups. The WEMC+DSS when compared with the DSS group had significantly higher catalase (272.9±54.8, 175.1+12.5, and 200.2±29.7), superoxide dismutase–SOD (347.7±37.5, 223.9±13.1, and 254.9±38.8), and glutathione peroxidase–GPx (28.6±2.20, 27.3±1.24, and 27.8±1.57) activities, and SOD/ (catalase+GPX) ratio (0.01±0.00, 0.003±0.00, and 0.01±0.002) and reduced hydrogen peroxide concentration (7.63±0.54, 4.33±1.28, and 5.71±0.75) in both colon and brain cytosolic fractions, respectively. Histological examination showed transmural inflammation in the DSS group as against mild mucosal inflammation in WEMC+DSS. This result shows that water extract of Momordica charantia has the potential to reduce colitis-induced oxidative damage and inflammation. Based on the results of this study, it can be concluded that the water extract of Momordica charantia leaves has the potential to mitigate oxidative damage and inflammation induced by ulcerative colitis in male Balb/c mice. Although there was no significant difference in disease activity indices among the groups, the group treated with the water extract of Momordica charantia before DSS administration showed significantly higher levels of antioxidant enzyme activities and lower levels of hydrogen peroxide concentration in both colon and brain cytosolic fractions compared to the DSS group.
Graphical Abstract
 Re ... ', 'data/ijabbr/coversheet/481713706812.png'))
Keywords
Main Subjects
OPEN ACCESS
©2024 The author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit: http://creativecommons.org/licenses/by/4.0/
PUBLISHER NOTE
Sami Publishing Company remains neutral concerning jurisdictional claims in published maps and institutional affiliations.
CURRENT PUBLISHER
- Halilu EM. Cultivation and conservation of african medicinal plants for pharmaceutical research and socio-economic development. inmedicinal Plants 2022 Nov 2. IntechOpen. [Crossref], [Google Scholar], [Publisher]
- Aware CB, Patil DN, Suryawanshi SS, Mali PR, Rane MR, Gurav RG, Jadhav JP. Natural bioactive products as promising therapeutics: A review of natural product-based drug development. South African Journal of Botany. 2022 Dec 1;151:512-28. [Crossref], [Google Scholar], [Publisher]
- Palamthodi S, Lele SS. Nutraceutical applications of gourd family vegetables: Benincasa hispida, Lagenaria siceraria and Momordica charantia. Biomedicine & Preventive Nutrition. 2014 Jan 1;4(1):15-21. [Crossref], [Google Scholar], [Publisher]
- Lee SY, Wong WF, Dong J, Cheng KK. Momordica charantia suppresses inflammation and glycolysis in lipopolysaccharide-activated RAW264. 7 macrophages. Molecules. 2020 Aug 20;25(17):3783. [Crossref], [Google Scholar], [Publisher]
- Dandawate PR, Subramaniam D, Padhye SB, Anant S. Bitter melon: a panacea for inflammation and cancer. Chinese Journal of Natural Medicines. 2016 Feb 1;14(2):81-100. [Crossref], [Google Scholar], [Publisher]
- Liaw CC, Huang HC, Hsiao PC, Zhang LJ, Lin ZH, Hwang SY, Hsu FL, Kuo YH. 5β, 19-epoxycucurbitane triterpenoids from Momordica charantia and their anti-inflammatory and cytotoxic activity. Planta Medica. 2014 Dec 3:62-70. [Crossref], [Google Scholar], [Publisher]
- Egberg MD, Kappelman MD, Gulati AS. Improving care in pediatric inflammatory bowel disease. Gastroenterology Clinics. 2018 Dec 1;47(4):909-19. [Crossref], [Google Scholar], [Publisher]
- Crowley E, Muise A. Inflammatory bowel disease: what very early onset disease teaches us. Gastroenterology Clinics. 2018 Dec 1;47(4):755-72. [Crossref], [Google Scholar], [Publisher]
- Coward S, Kaplan GG, IBD in the new world, old world, and your world. Inflammatory Bowel Disease: Diagnosis and Therapeutics, 2017;13-27. [Crossref], [Google Scholar], [Publisher]
- Chang M, Chang L, Chang HM, Chang F. Intestinal and extraintestinal cancers associated with inflammatory bowel disease. Clinical Colorectal Cancer. 2018 Mar 1;17(1): e29-37. [Crossref], [Google Scholar], [Publisher]
- De Souza HS, Fiocchi C, Iliopoulos D, The IBD interactome: an integrated view of aetiology, pathogenesis and therapy. Nature Reviews Gastroenterology & Hepatology, 2017;14:739-749. [Crossref], [Google Scholar], [Publisher]
- Wirtz S, Popp V, Kindermann M, Gerlach K, Weigmann B, Fichtner-Feigl S, Neurath MF, Chemically induced mouse models of acute and chronic intestinal inflammation. Nature Protocols, 2017; 12:1295-1309. [Crossref], [Google Scholar], [Publisher]
- Al-Laith AA, Alkhuzai J, Freije A. Assessment of antioxidant activities of three wild medicinal plants from Bahrain. Arabian Journal of Chemistry. 2019 Dec 1;12(8):2365-71. [Crossref], [Google Scholar], [Publisher]
- Tian T, Wang Z, Zhang J, Pathomechanisms of oxidative stress in inflammatory bowel disease and potential antioxidant therapies. Oxidative Medicine and Cellular Longevity, 2017;2017. [Crossref], [Google Scholar], [Publisher]
- Mohammed A, Tajuddeen N. Antidiabetic compounds from medicinal plants traditionally used for the treatment of diabetes in Africa: A review update (2015–2020). South African Journal of Botany. 2022 May 1; 146:585-602. [Crossref], [Google Scholar], [Publisher]
- Zhu QL, Guo SN, Wen F, Zhang XL, Wang CC, Si LF, Zheng JL, Liu J. Transcriptional and physiological responses of Dunaliella salina to cadmium reveals time-dependent turnover of the ribosome, photosystem, and ROS-scavenging pathways. Aquatic Toxicology. 2019 Feb 1; 207:153-62. [Crossref], [Google Scholar], [Publisher]
- Kim SK, Jung J, Jung JH, Yoon N, Kang SS, Roh GS, Hahm JR. Hypoglycemic efficacy and safety of Momordica charantia (bitter melon) in patients with type 2 diabetes mellitus. Complementary Therapies in Medicine. 2020 Aug 1; 52:102524. [Crossref], [Google Scholar], [Publisher]
- Wang S, Li Z, Yang G, Ho C.T, Li S, Momordica charantia: a popular health-promoting vegetable with multifunctionality. Food & Function, 2017;8:1749-1762. [Crossref], [Google Scholar], [Publisher]
- Opstelten JL, de Vries JH, Wools A, Siersema PD, Oldenburg B, Witteman BJ. Dietary intake of patients with inflammatory bowel disease: A comparison with individuals from a general population and associations with relapse. Clinical Nutrition. 2019 Aug 1;38(4):1892-8. [Crossref], [Google Scholar], [Publisher]
- Håkansson, Å., Tormo-Badia, N., Baridi, A., Xu, J., Molin, G., Hagslätt, M. L., ... & Ahrné, S. (2015). Immunological alteration and changes of gut microbiota after dextran sulfate sodium (DSS) administration in mice. Clinical and Experimental Medicine, 15, 107-120. [Crossref], [Google Scholar], [Publisher]
)
