Document Type : Original Article

Authors

• K. Moradpour ¹
• A. Najaphy ²
• S. Mansoorifar ²
• A. Mostafaie ³

¹ Graduate MSc of Agricultural Biotechnology, Razi University, Kermanshah, Iran

² Assistant Professor, Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, Razi University, Kermanshah, Iran

³ Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Abstract

Drought is one of the most important factors limiting crop yields around the world. Drought stress in plants, the change (increase or decrease) in the production of plant proteins. This research was carried out using bread wheat genotypes. For evaluation of leaf protein pattern in wheat, 10 genotypes were assayed with 3 replications under irrigated (non-stress) and rain-fed (stress) conditions. At grain filling stage, 10 random plants were selected and flag leaf samples were harvested. SDS-PAGE Electrophoresis was used to evaluate protein pattern after applying water stress. Thirty five protein bands appeared. Most of the bands were similar in the entries and specific bands were rare. Under drought stress, high molecular weight proteins were intensified, while low molecular weight proteins were faint. Cluster analysis under non-stress conditions classified the genotypes into tree clusters but under stress conditions the entries were classified into four clusters.

Keywords

• Drought stress
• electrophoresis
• Protein pattern
• Wheat