Identification of Probiotic Strains from Adesmia Cancellata Microbiomes for Vitamin B6 Production: A New Approach to Developing Functional Foods

Rahnama, Ali; Abolmaali, Shamsozoha; Darvish Alipour Astaneh, Shakiba

doi:10.48309/ijabbr.2026.2068455.1647

Identification of Probiotic Strains from Adesmia Cancellata Microbiomes for Vitamin B6 Production: A New Approach to Developing Functional Foods

Document Type : Original Article

Authors

Ali Rahnama ¹

Shamsozoha Abolmaali ²

Shakiba Darvish Alipour Astaneh ¹

¹ Department of Biotechnology, Faculty of New Sciences and Technologies, Semnan University, Semnan, Iran

² Department of Biology, Faculty of Basic Science, Semnan University, Semnan, Iran

https://doi.org/10.48309/ijabbr.2026.2068455.1647

Abstract

Probiotics are beneficial bacteria that produce specific metabolites and influence the host's health. Isolates from the intestinal microbiome of Adesmia cancellata were evaluated for probiotic properties. Then, quantitative and qualitative analyses of vitamins B2, B3, and B6 production in the cell-free supernatants (CFS) of probiotic strains from gut microbiota, local dairy products, and human breast milk were conducted using thin-layer chromatography (TLC), spectrophotometry, spectrofluorophotometry, and high-performance liquid chromatography (HPLC). Additionally, the probiotic strains effects on eukaryotic cells were examined to assess toxicity against human alveolar basal epithelial adenocarcinoma cells (A549) and their ability to adhere. Among the eight strains, DDMiCC12c, DDMiCC2Bk, and DDMiCC2D showed notable probiotic function in gut microbiota. DDMiCC2Bk demonstrated the strongest biofilm formation and the highest co-aggregation against Salmonella typhimurium. DDMiCC12c was the most resistant to acidic and bile conditions and exhibited co-aggregation with Bacillus subtilis. DDMiCC2D showed the highest auto-aggregation and co-aggregation against Staphylococcus aureus. Spectrofluorophotometric analysis confirmed the production of vitamin B2 in CFS, ranging from 5 to 10 mg/ml, while vitamin B6 concentrations were 15 to 20 mg/ml. HPLC analysis revealed the highest production of vitamin B6 in DDMiCC2D. SUBCC57 was the producer of vitamins B2 and B3. The selected probiotic strains maintained high viability for A549 cells, with SUBCC57 showing the lowest adhesion and SUBCC2 showing the highest adhesion to A549 cells. Incorporating probiotics into food products is a promising strategy to enhance both health and innovation in the food industry.

Graphical Abstract

Keywords

Adesmia Cancellata

B Vitamins

Gut Microbiome

Probiotic Characteristics

Probiotic Metabolites

Tenebrionidae

Subjects

Microbiology

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