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Effect of Vitamin E –Selenium Administered During Estrus Synchronization on Antioxidant Activity, Hormonal Profile, and Lamb Rearing of Moghani Ewes
Document Type : Original Article
Authors
1 Department of Animal Science, Moghan College of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
2 Department of Animal Science, Faculty of Agriculture, Payame Noor University, Tehran, Iran
3 Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Iran
Abstract
The study was conducted with 200 mature, non-lactating ewes aged 2–5 years and weighing about 70 kg, during the non-breeding season under natural photoperiod conditions. In the control group, only estrus synchronization was performed. The second group received two intramuscular injections (5 mL total) of E-Se (selenium as sodium selenite at 0.5 mg/mL and vitamin E as DL-α-tocopheryl at 50 IU) on the days of sponge insertion and sponge removal. Estrus synchronization involved placing intravaginal sponges for 14 days, followed by 400 IU eCG at sponge withdrawal. Two days after sponge withdrawal, ewes were mated with fertile rams. Three weeks after ram exposure, 20 ewes from each group were randomly selected for blood sampling. Hormone concentrations and antioxidant activity were measured. After parturition, reproductive traits were recorded for each group. The results showed that there was no significant effect of E-Se on the Lamb body weight at birth, body weight at 90 days, total weight gain and average daily gain. In E-Se treated group, number of lambs/ewe was significantly different (P < 0.05) compared with control. Progesterone concentration in E-Se groups was greater (P < 0.05) than the control. E-Se supplementation exerts a significant (p < 0.05) increasing effect on serum triiodothyronine (T3) concentration and the ratio of T3/T4. Total antioxidant capacity 3 weeks after ram exposure was significantly higher (P < 0.05) in E-Se than control. A significant (p < 0.05) increase in serum glutathione peroxidase concentration was observed in ewes supplemented with E-Se compared with the control group. Selenium concentration of serum was significantly higher in group receiving E-Se three weeks after mating (P < 0.05). However, there were no significant differences in serum malondialdehyde, superoxide dismutase, and hemoglobin concentrations between the E-Se and control groups. In summary, administering vitamin E-Se enhanced reproductive efficiency, antioxidant status, and serum progesterone levels in estrus-synchronized Moghani ewes during the non-breeding season.
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[1] Scaramuzzi, R.J., Campbell, B.K., Downing, J.A., Kendall, N.R., Khalid, M., Muñoz-Gutiérrez, M., Somchit, A., A review of the effects of supplementary nutrition in the ewe on the concentrations of reproductive and metabolic hormones and the mechanisms that regulate folliculogenesis and ovulation rate. Reproduction Nutrition Development, 2006, 46(4), 339-354.
[2] Naqvi, S., Soren, N., Karim, S., Effect of concentrate supplementation on performance, ovarian response, and some biochemical profile of malpura ewes. Tropical Animal Health and Production, 2011, 43(5), 905-913.
[3] Harrison, J.H., Hancock, D.D., Conrad, H., Vitamin E and selenium for reproduction of the dairy cow. Journal of Dairy Science, 1984, 67(1), 123-132.
[4] Agarwal, A., Gupta, S., Sharma, R.K., Role of oxidative stress in female reproduction. Reproductive Biology and Endocrinology, 2005, 3(1), 28.
[5] Kuru, M., Ögün, M., Oral, H., Kükürt, A., Makav, M., Kulaksız, R., The use of controlled internal drug release for synchronization augmented oxidative and nitrosative stress and leptin levels in georgian goats. J Cell Neurosci Oxid Stress, 2016, 8(1), 541-542.
[6] Martinez-Ros, P., Lozano, M., Hernandez, F., Tirado, A., Rios-Abellan, A., López-Mendoza, M.C., Gonzalez-Bulnes, A., Intravaginal device-type and treatment-length for ovine estrus synchronization modify vaginal mucus and microbiota and affect fertility. Animals, 2018, 8(12), 226.
[7] Al-Gubory, K.H., Fowler, P.A., Garrel, C., The roles of cellular reactive oxygen species, oxidative stress and antioxidants in pregnancy outcomes. The International Journal of Biochemistry & Cell Biology, 2010, 42(10), 1634-1650.
[8] Surai, P.F., Cardoso, D., Selenium in feed: Organic selenium concept development and its applications. Selenium in Ruminant Nutrition and Health, 2024, 329.
[9] Gavidel, N., Seifdavati, J., Abdibenemar, H., SeyedSharifi, R., The effect of colloid and micelles vs. Inorganic forms of nano-selenium on performance, health, growth factors, and some blood parameters of holstein calves. International Journal of Advanced Biological and Biomedical Research, 2025, 13, 284-298.
[10] El-Shahat, K., Abdel Monem, U., Effects of dietary supplementation with vitamin E and/or selenium on metabolic and reproductive performance of egyptian baladi ewes under subtropical conditions. World Applied Sciences Journal, 2011, 12(9), 1492-1499.
[11] Samimi, A.S., Nazifi, S., Asadi, Z., Bakhshaei, F., Farhang, A., Karamizadeh, F., Aghamiri, S.M., Effects of vitamin E/selenium and ad3e on progesterone, antioxidant enzymes and pregnancy rate of laparoscopic artificial insemination in sheep. Research Journal of Livestock Science, 2023, 36(138), 77-86.
[12] Farahavar, A., Rostami, Z., Alipour, D., Ahmadi, A., The effect of pre-breeding vitamin e and selenium injection on reproductive performance, antioxidant status, and progesterone concentration in estrus-synchronized mehraban ewes. Tropical Animal Health and Production, 2020, 52(4), 1779-1786.
[13] Vahedi, V., Mabodi, S., Yalchi, T., Ansari, M., The effect of vitamin e and selenium injection on the reproductive performance, birth weight and some blood metabolites in estrus-synchronized moghani ewes. Journal of Animal Production, Print ISSN: 2008-6776., Online ISSN: 2382-994X, 2023, 25(4), 473-483.
[14] Musa, S.I., Bitto, I.I., Ayoade, J.A., Oyedipe, O.E., Effects of vitamin e and selenium on fertility and lamb performance of yankasa sheep. Open Journal of Veterinary Medicine, 2018, 8(09), 167.
[15] Awawdeh, M., Eljarah, A., Ababneh, M., Multiple injections of vitamin e and selenium improved the reproductive performance of estrus-synchronized awassi ewes. Tropical Animal Health and Production, 2019, 51(6), 1421-1426.
[16] Gabryszuk, M., Klewiec, J., Effect of injecting 2-and 3-year-old ewes with selenium and selenium–vitamin e on reproduction and rearing of lambs. Small Ruminant Research, 2002, 43(2), 127-132.
[17] Esterbauer, H., Cheeseman, K.H., [42] Determination of aldehydic lipid peroxidation products: Malonaldehyde and 4-hydroxynonenal. Methods in Enzymology, 1990, 186, 407-421.
[18] El-Ghany-Hefnawy, A., López-Arellano, R., Revilla-Vázquez, A., Ramírez-Bribiesca, E., Tórtora-Pérez, J., The relationship between fetal and maternal selenium concentrations in sheep and goats. Small Ruminant Research, 2007, 73, 174-180.
[19] Zanao, R.A., Barbosa Jr, F., Souza, S.S., Krug, F.J., Abdalla, A.L., Direct determination of selenium in whole blood by electrothermal atomic absorption spectrometry using w–rh-coated platform and co-injection of rh as thermal stabilizer. Spectrochimica Acta Part B: Atomic Spectroscopy, 2002, 57(2), 291-301.
[20] Koyuncu, M., Yerlikaya, H., Effect of selenium-vitamin e injections of ewes on reproduction and growth of their lambs. South African Journal of Animal Science, 2007, 37(4), 233-236.
[21] Basini, G., Tamanini, C., Selenium stimulates estradiol production in bovine granulosa cells: Possible involvement of nitric oxide☆. Domestic Animal Endocrinology, 2000, 18(1), 1-17.
[22] Sánchez, J., Jiménez, A., Regodón, S., Andrés, S., Inhibitory effect of selenium supplementation on the reproductive performance in synchronized merino sheep at range conditions in a selenium‐deficient area. Reproduction in Domestic Animals, 2008, 43(3), 328-332.
[23] Hemingway, R., Parkins, J., Ritchie, N., Enhanced reproductive performance of ewes given a sustained-release multi-trace element/vitamin ruminal bolus. Small Ruminant Research, 2001, 39(1), 25-30.
[24] Munoz, C., Carson, A., McCoy, M., Dawson, L., Irwin, D., Gordon, A., Kilpatrick, D., Effect of supplementation with barium selenate on the fertility, prolificacy and lambing performance of hill sheep. Veterinary Record, 2009, 164(9), 265-271.
[25] Ramírez-Bribiesca, J., Tórtora, J., Huerta, M., Hernández, L., López, R., Crosby, M., Effect of selenium-vitamin e injection in selenium-deficient dairy goats and kids on the mexican plateau. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2005, 57, 77-84.
[26] Pieczyńska, J., Grajeta, H., The role of selenium in human conception and pregnancy. Journal of trace Elements in Medicine and Biology, 2015, 29, 31-38.
[27] Pappas, A., Zoidis, E., Surai, P., Zervas, G., Selenoproteins and maternal nutrition. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2008, 151(4), 361-372.
[28] Yıldız, A., Balikçi, E., Gürdoğan, F., Effect of injection of vitamin e and selenium administered immediately before the ovsynch synchronization on conception rate, antioxidant activity and progesterone levels in dairy cows. Firat Universitesi Saglık Bilimleri Dergisi, 2015, 29, 183-186.
[29] Kamada, H., Effects of selenium-rich yeast supplementation on the plasma progesterone levels of postpartum dairy cows. Asian-Australasian Journal of Animal Sciences, 2016, 30(3), 347.
[30] Cam, M.A., Kuran, M., GnRH agonist treatment on day 12 post-mating to improve reproductive performance in goats. Small Ruminant Research, 2004, 52(1-2), 169-172.
[31] Lekatz, L., Caton, J., Taylor, J., Reynolds, L., Redmer, D., Vonnahme, K., Maternal selenium supplementation and timing of nutrient restriction in pregnant sheep: Effects on maternal endocrine status and placental characteristics. Journal of Animal Science, 2010, 88(3), 955-971.
[32] Zagrodzki, P., Ratajczak, R., Selenium status, sex hormones, and thyroid function in young women. Journal of Trace Elements in Medicine and Biology, 2008, 22(4), 296-304.
[33] Carlson, J.C., Sawada, M., Boone, D.L., Stauffer, J.M., Stimulation of progesterone secretion in dispersed cells of rat corpora lutea by antioxidants. Steroids, 1995, 60(3), 272-276.
[34] Abou-Zeina, H., Hamam, A., Effect of parental administration of vitamin e and or selenium on health and reproductive function of ewes fed on marginal deficient diets. Egyptian Journal of Basic and Applied Physiology, 2002, 1, 205-223.
[35] Alimohamady, R., Aliarabi, H., Bahari, A., Dezfoulian, A.H., Influence of different amounts and sources of selenium supplementation on performance, some blood parameters, and nutrient digestibility in lambs. Biological Trace Element Research, 2013, 154(1), 45-54.
[36] Kumar, N., Garg, A., Dass, R., Chaturvedi, V., Mudgal, V., Varshney, V., Selenium supplementation influences growth performance, antioxidant status and immune response in lambs. Animal Feed Science and Technology, 2009, 153(1-2), 77-87.
[37] Rock, M., Kincaid, R., Carstens, G., Effects of prenatal source and level of dietary selenium on passive immunity and thermometabolism of newborn lambs. Small Ruminant Research, 2001, 40(2), 129-138.
[38] Jung, D., Kim, D., Beak, S., Cho, I., Hong, S., Lee, J., Lee, J., Kim, H., Malekkhahi, M., Baik, M., Effects of vitamin e and selenium administration on transportation stress in pregnant dairy heifers. Journal of Dairy Science, 2023, 106(12), 9576-9586.
[39] Mahmood, N., Hameed, A., Hussain, T., Vitamin e and selenium treatment alleviates saline environment‐induced oxidative stress through enhanced antioxidants and growth performance in suckling kids of beetal goats. Oxidative Medicine and Cellular Longevity, 2020, 2020(1), 4960507.
[40] Nurlatifah, A., Astuti, D.A., Herdis, H., Arifiantini, I., Pamungkas, F.A., Santoso, S., Diapari, D., Sitaresmi, P.I., Setiatin, E.T., Diansyah, A.M., Mitigating heat stress in garut lambs: Synergistic effects of lemuru fish oil, vitamin e, and selenium on antioxidant defense, hematology, and physiological responses. Veterinary World, 2025, 18(8), 2230.
[41] Hamam, A., Abou-Zeina, H., Effect of vitamin e and selenium supplements on the antioxidant markers and immune status in sheep. Journal of Biological Sciences, 2007, 7, 870-878.
[42] Smith, O., Akinbamijo, O., Micronutrients and reproduction in farm animals. Animal Reproduction Science, 2000, 60, 549-560.
[43] Abdel-Raheem, S., Mahmoud, G., Senosy, W., El-Sherry, T., Influence of vitamin e and selenium supplementation on the performance, reproductive indices and metabolic status of ossimi ewes. Slovenian Veterinary Research, 2019, 56(Suppl 22), 353-363.
Volume 14, Issue 2
March and April 2026Pages 227-238
- Receive Date 16 September 2025
- Revise Date 22 October 2025
- Accept Date 19 November 2025
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