Document Type : Original Article


1 Graduate student, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

2 Department of Avian Medicine, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

3 Center of Comparative&experimental medicine, Basic sciences in infection diseases research center, School of medicine, Shiraz University of Medical Sciences, Shiraz, Iran

4 School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran


Infectious agents cause disease in virtually any susceptible hosts. In the poultry production and medicine, pathogens were eliminated from a line of a breeder by injection of antibiotics into hatching eggs. There is little information available describing the safety of the folate antimetabolite/sulfonamide class of antibiotics on the game birds embryonated eggs. The objective of this study was to investigate the efficiency of the injection oftrimethoprim/sulfamethoxazole solution on the partridge embryonated egg. Fertile partridge eggs were distributed into 3 groups and set in the incubator. On 18th day post incubation, one group was injected with the trimethoprim/sulfamethoxazole injectable solution dissolved in 0.3 ml phosphate buffered saline. Rest two groups were used as sham control (0.3 ml phosphate buffered saline solution) and un-injected control. Macroscopic evaluationon 21th day post incubation showed that embryos were normal in all treatment groups. Microscopically, no lesions were also diagnosed in the brain, heart, muscle, liver, kidney and lung of the embryos. Based on macroscopic and microscopic findings, it is concluded that trimethoprim/sulfamethoxazole at above-mentioned concentration is not toxic for the partridge embryo at the late stage of development. So, trimethoprim/sulfamethoxazole egg-injection can be used toeliminate pathogens and prevention of egg transmission of the diseasewithout any adverse effect.


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