Document Type : Original Article


Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran


κ-casein is a glycosilated protein in mammalian milk that plays an essential role in the milk micelles. Control of κ-casein expression reflects this essential role, although an understanding of the mechanisms involved lags behind that of the other milk protein genes. Transcriptional regulation, a first mechanism for controlling the development of organisms, is carried out by transcription factors binding sites (TFBSs).  We determined the 5’ region for Iranian Dromedaries and bactrianus camel κ-casein gene. We also determine their regulatory TFBSs regions. This region contained 14 potential TFBSs The four of them C/EBP-α, Oct1, MGF/STAT5 and TPB have almost perfectly conserved.


Yahyaoui M.H., Angiolillo A., Pilla F., Sanchez A. and Folch, J.M. Characterization and genotyping of the caprine j-casein variants. Journal of Dairy Science. 86, 2715–20, 2003.
Farrell Jr H. M., Jimenez-Flores R and Bleck G. T. Nomenclature of the proteins of cows’ milk sixth revision,” Journal of Dairy Science, vol. 87, no. 6, pp. 1641–1674, 2004.
Heck J. M. L., Schennink A., van Valenberg H. J. F., Effects of milk protein variants on the protein composition of bovine milk, Journal of Dairy Science, vol. 92, no. 3, pp. 1192–1202, 2009.
Prinzenberg E. M., Weimann C., Brandt H., Polymorphism of the bovine CSN1S1 promoter: linkage mapping, intragenic haplotypes and effects on milk production traits. Journal of Dairy Science, vol. 86, no. 8, pp. 2696–2705, 2003.
Szymanowska M., Strzalkowska N., Siadkowska E., Krzyzewski J., Ryniewicz Z and Zwierzchowski L. Effects of polymorphism at 5′-noncoding regions (promoters) of α-S1- and α- S2-casein genes on selected milk production traits in Polish Black-and White cows. Animal Science Papers and Reports, vol. 21, pp. 97–108, 2003.
Malewski T. Computer analysis of distribution of putative cisand trans- regulatory elements in milk protein gene promoters. BioSystems, vol. 45, no. 1, pp. 29–44, 1998.
Prinzenberg E. M., Brandt H., Bennewitz J., Kalm E and G. Erhardt, Allele frequencies for SNPs in the αs1-casein gene (CSN1S1) 5′ αs1 flanking region in European cattle and association with economic traits in German Holstein. Livestock Production Science, vol. 98, no. 1-2, pp. 155–160,
Kuss A. W., Gogol J., Bartenschlager. H and H. Geldermann. Polymorphic binding site in bovine CSN1S1 shows quantitative differences in protein binding associated with milk protein expression.
Journal of Dairy Science, vol. 88, no. 6, pp. 2246–2252, 2005.
Bai W. L., Yin R. H., Dou Q. L. A single nucleotide polymorphism and sequence analysis of CSN1S1 gene promoter region in Chinese BOS Grunniens (YAK). Animal Biotechnology, vol. 21, o. 1, pp. 36–41, 2010.
Chianese L., Quarto M., Pizzolongo F. Occurrence of genetic polymorphism at the αs1-casein locus in Mediterranean water buffalo milk. International Dairy Journal, vol. 19, no. 4, pp. 181– 189, 2009.
Ramunno L., Cosenza G., Rando A. the goat αS1-casein gene: gene structure and promoter analysis. Gene, vol. 334, no. 1-2, pp. 105–111, 2004.
Bhure S. K. and B. Sharma. the PCR amplification, sequencing and computer-aided analysis of ovine αS1-casein gene promoter, Journal of Biotechnology, vol. 7, no. 4, pp. 478–481, 2008.
Pauciullo A., Shuiep E., Cosenza G., Ramunno L. and Erhardt G. Molecular characterization and genetic variability at κ-casein gene (CSN3) in camels. Gene, 513(1), 22-30, 2013.
Gerencsér, A., Barta, E., Boa, S., Kastanis, P., Bosze, Z., Whitelaw, C.B. Comparative analysis on the structural features of the 5’ flanking region of k-casein genes from six different species. Genet. Sel. Evol. 34, 117–128, 2002.
Hobor, S., Kunej, T., Dovc, P. Polymorphisms in the kappa casein (CSN3) gene in horse and comparative analysis of its promoter and coding region. Anim. Genet. 39, 520–530, 2008.
Malewski, T. Computer analysis of distribution of putative cis- and trans regulatory elements in milk protein gene promoters. Biosystems 45, 29–44, 1998.
Vanselow A. DNA-remethylation around a STAT5-binding enhancer in the alphaS1-casein promoter is associated with abrupt shutdown of alphaS1-casein synthesis during acute mastitis. Endocrinol. 37, 463–477, 2006.
Zhao, F.Q., Adachi, K., Oka, K., 2002. Involvement of Oct-1 in transcriptional regulation of β-casein gene expression in mouse mammary gland. Biochim. Biophys. Acta 1577, 27–37.
Zwilling, S., Annweiler, A., Wirth, T., 1994. The POU domains of the Oct-1 and Oct-2 transcription factors mediate specific interaction with TBP. Nucleic Acids Res. 22, 1655–1662.
Akos Gerencser, Endre Barta, Simon Boa, Petros Kastanis, Zsuzsanna Bosze, C. Bruce and A.Whitelaw. 2001. Comparative analysis on the stuctural features of the 5' flanking region of kcasein genes from six different species. Genet.Sel Evol., 34: 117-128.