Document Type : Original Article
Authors
- Hojjat Asadollahpour Nanaei
- Saeid Ansari Mahyari
- Mohammad-Ali Edriss
- Mahmood Rostami
- Malihe Pirzad
- Amir Boroushak
Dep. of Animal Science, College of Agriculture, Isfahan University of Technology, Isfahan, Iran
Abstract
In this study we estimated the allele and genotype frequencies of SCD1 and DGAT1 gene polymorphism. The analysis was conducted on 408 Holstein cows from five dairy herds in Isfahan province. Genotypes were identified using PCR-RFLP technique. Two genetic variants (A and V) of the SCD1 gene were observed in this experiment. The frequency of A-allele ranged from 0.49 to 0.66, while frequency of V-allele 0.34 to 0.51. Regards with DGAT1 gene, also two genetic variants (A and K) were determined that frequency of A-allele ranged from 0.54 to 0.68 and K-allele 0.32 to 0.46. It was found that these two genes were polymorphic in Isfahan Holstein cows, which suggested that could be associated with composition and production traits
Keywords
[1] Boichard D, Grohs C, Bourgeois F, Cerqueira F, Faugeras R, Neau A, Rupp R, Amigues Y, Boscher M Y, Leveziel H. Detection of genes influencing economic traits in three French dairy cattle breeds. Genet. Sel. Evol. 2003; 35:77–101.
[2] Campbell EMG, Callagher DS, Davis SK, Taylor JF, Smith SB. Mapping of the bovine stearoylcoenzyme A desaturase (SCD) gene to BTA26. J. Anim. Sci. 2001; 79:1954–1955.
[3] Coppieters W, Riquet J, Arranz JJ, Berzi P, Cambisano N, Grisart B, Karim L, Marcq F, Moreau L, Nezer C, Simon P, Vanmanshoven P, Wagenaar D, Georges M. A QTL with major effect on milk yield and composition maps to bovine chromosome 14. Mammalian Genome. 1998; 9, 540-544.
[4] Dobrzyn A, Ntambi JM. The role of stearoyl–CoA desaturase in the control of metabolism. Prostaglandins Leukot Essent Fatty Acids. 2005; 73:35–41.
[5] Druet T, Fritz S, Boichard D, Colleau JJ. Estimation of genetic parameters for quantitative trait loci for dairy traits in the French Holstein population. J. Dairy Sci. 2006; 89:4070–4076.
[6] Flowers MT, Ntambi JM. Role of stearoyl–coenzyme A desaturase in regulating lipid metabolism. Curr Opin Lipidol. 2008. 19: 248–256.
[7] Gautier M, Capitan A, Fritz S, Eggen A, Boichard D, Druet T. Characterization of the DGAT1 K232A and variable number of tandem repeat polymorphisms in French dairy cattle. Journal of Dairy Science. 2007; 90, 2980-2988.
[8] Grisart B, Coppieters W, Farnir F, Karim L, Ford C, Berzi P, Cambisano N, Mni M, Reid S, Simon P, Spelman R, Georges M, Snell R. Positional candidate cloning of a QTL in dairy cattle: Identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition.
Genome Research. 2002; 12, 222-231.
[9] Heyen DW, Weller JI, Ron M, Band M, Beever JE, Feldmesser E, Da Y, Wiggans GR, Vanraden PM, Lewin HA. A genome scan for QTL influencing milk production in dairy cattle. Physiological Genomics. 1999; 1, 165-175.
[10] Kauppe B, Winter A, Fries R, Erhardt G. DGAT1 polymorphism in Bos indicus and Bos taursus cattle breeds. Journal of Dairy Research. 2004; 71, 182-187.
[11] Kgwatalala PM, Ibeagha-Awemu, EM, Mustafa, A F, Zhao, X. Stearoyl-CoA desaturase 1 genotype and stage of lactation influences milk fatty acid composition of Canadian Holstein cows. Animal Genetics. 2009; 5, pages 609–615.
[12] Kharrati Koopaei H, Mohammad Abadi MR, Ansari Mahyari S, Esmailizadeh Koshkoiyeh A, Tarang AR, Potki P. Effect of DGAT1 variants on milk composition traits in Iranian Holstein cattle population. Animal Science Papers and Reports. 2012; 3, 231-239.
[13] Komisarek J, Waskowicz K, Michalak A, Dorynek Z. Effects of DGAT1 variants on milk production traits in Jersey cattle. Animal Science Papers and Reports. 2004; 3, 307-313.
[14] Lehner R, Kuksis A. Biosynthesis of triacylglycerols. Progress in Lipid Research. 1996; 35, 169-201.
[15] Macciotta NPP, Mele M, Conte G, Serra A, Cassandro M, Dal Zotto R, Cappio Borlino A, Pagnacco G, Secchiari P. Association Between a Polymorphism at the Stearoyl CoA Desaturase Locus and Milk Production Traits in Italian Holsteins. J. Dairy Sci. 2007; 91:3184–3189.
[16] Marshall MO, Knudsen J. Biosynthesis of triacylglycerols containing short-chain fatty acids in lactating cow mammary gland. Activity of diacylglycerol acyltransferase towards shortchain acyl-CoA esters. Journal of Biochemistry. 1977; 81, 259-266.
[17] Miller SA, Dykes DD, Polesky HF. A simple salting-out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research. 1988; 16, 1215-1219.
[18] Paton CM, Ntambi JM. Biochemical and physiological function of stearoyl-CoA desaturase. Am. J. Physiol-Endoc M 297, E 28–E37. 2009 Doi: 10.1152/ajpendo.90897.2008, http: //ajpendo. physiology.org.
[19] Plante Y, Gibson JP, Nadesalingam J, Mehrabani-Yeganeh H, Lefebvre S, Vandervoort G, Jansen GB. Detection of quantitative trait loci affecting milk production traits on 10 chromosomes in Holstein cattle. J. Dairy Sci. 2001; 84:1516–1524.
[20] Ripoli MV, Corva P, Giovambattita G. Analysis of a polymorphism in the DGAT1 gene in 14 cattle breeds through PCR-SSCP methods. Research Veterinary Science. 2006; 80, 287-290.
[21] Riquet J, Coppieters W, Cambisano N, Arranz JJ, Berzi P, Davis S, Grisart B, Farnir F, Karim L, Mni M, Simon P, Taylor J.F, Vanmanshoven P, Wagenaar D, Womack JE, Georges M. Identityby-decent fine-mapping of QTL in outbred populations: Application to milk production in dairy cattle.
Proceedings of the National Academy of Sciences, USA. 1999; 96, 9252-9257.
[22] Sanders K, Bennewitz J, Reinsch N, Thaller G, Prinzenberg EM, Kuhn C, Kalm E. Characterization of the DGAT1 mutations and the CSN1S1 promoter in the German Angeln dairy cattle population. Journal of Dairy Science. 2006; 89, 3164-3174.
[23] Spelman RJ, Ford CA, Mcelhinney P, Gregory GC, Snell RG. Characterization of the DGAT1 gene in the New Zealand dairy population. Journal of Dairy Science. 2002; 85, 3514-3517.
[24] Thaller G, Kramer W, Winter A, Kaupe B, Erhardt G, Fries R. Effects of DGAT1 variants on milk production traits in German cattle breeds. American Society of Animal Science. 2003; 81, 1911-1918.
[2] Campbell EMG, Callagher DS, Davis SK, Taylor JF, Smith SB. Mapping of the bovine stearoylcoenzyme A desaturase (SCD) gene to BTA26. J. Anim. Sci. 2001; 79:1954–1955.
[3] Coppieters W, Riquet J, Arranz JJ, Berzi P, Cambisano N, Grisart B, Karim L, Marcq F, Moreau L, Nezer C, Simon P, Vanmanshoven P, Wagenaar D, Georges M. A QTL with major effect on milk yield and composition maps to bovine chromosome 14. Mammalian Genome. 1998; 9, 540-544.
[4] Dobrzyn A, Ntambi JM. The role of stearoyl–CoA desaturase in the control of metabolism. Prostaglandins Leukot Essent Fatty Acids. 2005; 73:35–41.
[5] Druet T, Fritz S, Boichard D, Colleau JJ. Estimation of genetic parameters for quantitative trait loci for dairy traits in the French Holstein population. J. Dairy Sci. 2006; 89:4070–4076.
[6] Flowers MT, Ntambi JM. Role of stearoyl–coenzyme A desaturase in regulating lipid metabolism. Curr Opin Lipidol. 2008. 19: 248–256.
[7] Gautier M, Capitan A, Fritz S, Eggen A, Boichard D, Druet T. Characterization of the DGAT1 K232A and variable number of tandem repeat polymorphisms in French dairy cattle. Journal of Dairy Science. 2007; 90, 2980-2988.
[8] Grisart B, Coppieters W, Farnir F, Karim L, Ford C, Berzi P, Cambisano N, Mni M, Reid S, Simon P, Spelman R, Georges M, Snell R. Positional candidate cloning of a QTL in dairy cattle: Identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition.
Genome Research. 2002; 12, 222-231.
[9] Heyen DW, Weller JI, Ron M, Band M, Beever JE, Feldmesser E, Da Y, Wiggans GR, Vanraden PM, Lewin HA. A genome scan for QTL influencing milk production in dairy cattle. Physiological Genomics. 1999; 1, 165-175.
[10] Kauppe B, Winter A, Fries R, Erhardt G. DGAT1 polymorphism in Bos indicus and Bos taursus cattle breeds. Journal of Dairy Research. 2004; 71, 182-187.
[11] Kgwatalala PM, Ibeagha-Awemu, EM, Mustafa, A F, Zhao, X. Stearoyl-CoA desaturase 1 genotype and stage of lactation influences milk fatty acid composition of Canadian Holstein cows. Animal Genetics. 2009; 5, pages 609–615.
[12] Kharrati Koopaei H, Mohammad Abadi MR, Ansari Mahyari S, Esmailizadeh Koshkoiyeh A, Tarang AR, Potki P. Effect of DGAT1 variants on milk composition traits in Iranian Holstein cattle population. Animal Science Papers and Reports. 2012; 3, 231-239.
[13] Komisarek J, Waskowicz K, Michalak A, Dorynek Z. Effects of DGAT1 variants on milk production traits in Jersey cattle. Animal Science Papers and Reports. 2004; 3, 307-313.
[14] Lehner R, Kuksis A. Biosynthesis of triacylglycerols. Progress in Lipid Research. 1996; 35, 169-201.
[15] Macciotta NPP, Mele M, Conte G, Serra A, Cassandro M, Dal Zotto R, Cappio Borlino A, Pagnacco G, Secchiari P. Association Between a Polymorphism at the Stearoyl CoA Desaturase Locus and Milk Production Traits in Italian Holsteins. J. Dairy Sci. 2007; 91:3184–3189.
[16] Marshall MO, Knudsen J. Biosynthesis of triacylglycerols containing short-chain fatty acids in lactating cow mammary gland. Activity of diacylglycerol acyltransferase towards shortchain acyl-CoA esters. Journal of Biochemistry. 1977; 81, 259-266.
[17] Miller SA, Dykes DD, Polesky HF. A simple salting-out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research. 1988; 16, 1215-1219.
[18] Paton CM, Ntambi JM. Biochemical and physiological function of stearoyl-CoA desaturase. Am. J. Physiol-Endoc M 297, E 28–E37. 2009 Doi: 10.1152/ajpendo.90897.2008, http: //ajpendo. physiology.org.
[19] Plante Y, Gibson JP, Nadesalingam J, Mehrabani-Yeganeh H, Lefebvre S, Vandervoort G, Jansen GB. Detection of quantitative trait loci affecting milk production traits on 10 chromosomes in Holstein cattle. J. Dairy Sci. 2001; 84:1516–1524.
[20] Ripoli MV, Corva P, Giovambattita G. Analysis of a polymorphism in the DGAT1 gene in 14 cattle breeds through PCR-SSCP methods. Research Veterinary Science. 2006; 80, 287-290.
[21] Riquet J, Coppieters W, Cambisano N, Arranz JJ, Berzi P, Davis S, Grisart B, Farnir F, Karim L, Mni M, Simon P, Taylor J.F, Vanmanshoven P, Wagenaar D, Womack JE, Georges M. Identityby-decent fine-mapping of QTL in outbred populations: Application to milk production in dairy cattle.
Proceedings of the National Academy of Sciences, USA. 1999; 96, 9252-9257.
[22] Sanders K, Bennewitz J, Reinsch N, Thaller G, Prinzenberg EM, Kuhn C, Kalm E. Characterization of the DGAT1 mutations and the CSN1S1 promoter in the German Angeln dairy cattle population. Journal of Dairy Science. 2006; 89, 3164-3174.
[23] Spelman RJ, Ford CA, Mcelhinney P, Gregory GC, Snell RG. Characterization of the DGAT1 gene in the New Zealand dairy population. Journal of Dairy Science. 2002; 85, 3514-3517.
[24] Thaller G, Kramer W, Winter A, Kaupe B, Erhardt G, Fries R. Effects of DGAT1 variants on milk production traits in German cattle breeds. American Society of Animal Science. 2003; 81, 1911-1918.
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