MSRT,ISC
37
h-index
h-index
Effect of Stat1 Variants on Milk Production Traits in Esfahan Holstein Cows
Document Type : Original Article
Authors
1 Department of Animal Science, Isfahan University of Technology, Iran
2 Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, Iran
Abstract
STATs are a family of latent transcription factors that reside in the cytoplasm of resting cells. The STAT1 transcription factor gene located on the chromosome 2 at interval 60 to 63 cM. This factor upon stimulation with INF gamma, dimerizes and translocate to the nucleus where mediates transcriptional regulation. In this study, we estimated the allele and genotype frequencies of STAT1/Pag1 gene polymorphism and examined the association whit milk yield (305-day milk yield) and milk component (fat and protein percentage) traits. DNA was isolated from 317 Holstein cows of five different herds. A 314 bp fragment in STAT1 gene was amplified and the animals were genotyped using RFLP-PCR technique. Seven genotypes including DD (89 animals), BB (25 animals), CC (11 animals), AC (15 animals), BC (9 animals), CD (164 animals) and BD (4 animals) were identified. Frequencies of A, B, C, and D alleles were estimated to be 0.021, 0.101, 0.332 and 0.546, respectively. Association of STAT1/Pag1 genotypes with percentage of fat in the milk was relatively high (P < 0.05); the DD genotype was superior to other groups. But corrected milk production for 305 days and protein percentage of the milk did not differ among all groups (P > 0.01). We concluded that this marker should be considered for milk component (fat percentage) selection in Holstein dairy cows
Keywords
Asadollahpour Nanaei H, Ansari Mahyari S, Edriss MA, Pirzad M, Boroushak A (2013) Polymorphism of SCD1 and DGAT1 gene in Isfahan Holstein cows. Int J Adv Biol Biom Res, 1(7), 783-788.
Ashwell MS, Heyen DW, Sonstegard TS, VanTassell CP, Da Y, Vanraden PM, Ron M, Weller JI, Lewin HA (2004) Detection of quantitative trait loci affecting milk production,health, and reproductive traits in Holstein cattle. J Anim Sci 87, 468–475.
Band MR, Larson JH, Rebeiz M, Green CA, Heyen DW, Donovan J, Windish R, Steining C, Mahyuddin P, Womack JE, Lewin HA (2000) An ordered comparative map of the cattle and human genomes. Genome Res10, 1359–1368.
Catlett-Falcone R, Dalton WS, Jove R (1999) STAT proteins as novel targets for cancer therapy.Curr Opin Oncol 11, 490 – 496.
Cobanoglu O, Zaitoun I, Chang YM, Shook GE, Khatib H (2006) Effects of the Signal Transducer and Activator of Transcription 1 (STAT1) Gene on Milk Production Traits in Holstein Dairy Cattle. J Anim Sci 89, 4433–4437.
Gavrilescu LC, Butcher BA, Delrio L, Taylor GA, Denkers EY (2004) STAT1 Is Essential for Antimicrobial Effector Function but Dispensable for Gamma Interferon Production during Toxoplasma gondii Infection. Infect Immun 72, 1257–1264.
Khatib H, Huang W, Mikheil D, Schutzkus V, Monson RL (2009) Effects of signal transducer and activator of transcription (STAT) genes STAT1 and STAT3 genotypic combinations on fertilization and embryonic survival rates in Holstein cattle. J Anim Sci 92, 6186-91.
Klover PJ, Muller WJ, Robinson GW, Pfeiffer RM, Yamaji D, Hennighausen L (2010) Loss of STAT1 from mouse mammary epithelium results in an increased Neu-induced tumor burden. Neoplasia 12, 899–905.
Levy DE, Gilliland DG (2000) Divergent roles of STAT1 and STAT5 in malignancy as revealed by gene disruptions in mice. Oncogene 19, 2505 – 2510.
Mao J, Molenaar AJ, Wheeler TT, Seyfert HM (2002) STAT5 binding contributes to lactational stimulation of promoter III expressing the bovine acetyl-CoA carboxylase α-encoding gene in the mammary gland. J Mol Endocrinol 29, 73–88.
Miller SA, Dykes DD, Polesky HF (1988) A simple salting-out procedure for extracting DNA from human nucleated cells. Nucl Acids Res 16, 1215-1219.
Mosig MO, Lipkin E, Khutoreskaya G, Tchourzyna E, Soller M, Friedmann A (2001) A whole genome scan for quantitative trait loci affecting milk protein percentage in Israeli-Holstein cattle, by means of selective milk DNA pooling in a daughter design, using an adjusted false discovery rate criterion.
Genetics 157, 1683–1698.
Nei M (1977) F-statistics and analysis of gene diversity in subdivided populations. Ann Hum Genet 41, 225-233.
Ron M, Feldmesser E, Golik M, Tager-Cohen I, Kliger D, Reiss V, Domochovsky R, Alus O, Seroussi E, Ezra E, Weller JI (2004) A complete genome scan of the Israeli Holstein population for quantitative trait loci by a daughter design. J Anim Sci 87, 476–490.
SAS (Statistical Analysis Systems), 1997 – SAS-STAT User’s Guide, SAS Inst. Inc. Cary. NC.
Watson C J, Neoh k (2008) The Stat family of transcription factors have diverse roles in mammary gland development. Semin Cell Dev Biol 19, 401-6.
Ashwell MS, Heyen DW, Sonstegard TS, VanTassell CP, Da Y, Vanraden PM, Ron M, Weller JI, Lewin HA (2004) Detection of quantitative trait loci affecting milk production,health, and reproductive traits in Holstein cattle. J Anim Sci 87, 468–475.
Band MR, Larson JH, Rebeiz M, Green CA, Heyen DW, Donovan J, Windish R, Steining C, Mahyuddin P, Womack JE, Lewin HA (2000) An ordered comparative map of the cattle and human genomes. Genome Res10, 1359–1368.
Catlett-Falcone R, Dalton WS, Jove R (1999) STAT proteins as novel targets for cancer therapy.Curr Opin Oncol 11, 490 – 496.
Cobanoglu O, Zaitoun I, Chang YM, Shook GE, Khatib H (2006) Effects of the Signal Transducer and Activator of Transcription 1 (STAT1) Gene on Milk Production Traits in Holstein Dairy Cattle. J Anim Sci 89, 4433–4437.
Gavrilescu LC, Butcher BA, Delrio L, Taylor GA, Denkers EY (2004) STAT1 Is Essential for Antimicrobial Effector Function but Dispensable for Gamma Interferon Production during Toxoplasma gondii Infection. Infect Immun 72, 1257–1264.
Khatib H, Huang W, Mikheil D, Schutzkus V, Monson RL (2009) Effects of signal transducer and activator of transcription (STAT) genes STAT1 and STAT3 genotypic combinations on fertilization and embryonic survival rates in Holstein cattle. J Anim Sci 92, 6186-91.
Klover PJ, Muller WJ, Robinson GW, Pfeiffer RM, Yamaji D, Hennighausen L (2010) Loss of STAT1 from mouse mammary epithelium results in an increased Neu-induced tumor burden. Neoplasia 12, 899–905.
Levy DE, Gilliland DG (2000) Divergent roles of STAT1 and STAT5 in malignancy as revealed by gene disruptions in mice. Oncogene 19, 2505 – 2510.
Mao J, Molenaar AJ, Wheeler TT, Seyfert HM (2002) STAT5 binding contributes to lactational stimulation of promoter III expressing the bovine acetyl-CoA carboxylase α-encoding gene in the mammary gland. J Mol Endocrinol 29, 73–88.
Miller SA, Dykes DD, Polesky HF (1988) A simple salting-out procedure for extracting DNA from human nucleated cells. Nucl Acids Res 16, 1215-1219.
Mosig MO, Lipkin E, Khutoreskaya G, Tchourzyna E, Soller M, Friedmann A (2001) A whole genome scan for quantitative trait loci affecting milk protein percentage in Israeli-Holstein cattle, by means of selective milk DNA pooling in a daughter design, using an adjusted false discovery rate criterion.
Genetics 157, 1683–1698.
Nei M (1977) F-statistics and analysis of gene diversity in subdivided populations. Ann Hum Genet 41, 225-233.
Ron M, Feldmesser E, Golik M, Tager-Cohen I, Kliger D, Reiss V, Domochovsky R, Alus O, Seroussi E, Ezra E, Weller JI (2004) A complete genome scan of the Israeli Holstein population for quantitative trait loci by a daughter design. J Anim Sci 87, 476–490.
SAS (Statistical Analysis Systems), 1997 – SAS-STAT User’s Guide, SAS Inst. Inc. Cary. NC.
Watson C J, Neoh k (2008) The Stat family of transcription factors have diverse roles in mammary gland development. Semin Cell Dev Biol 19, 401-6.
Volume 1, Issue 8 - Serial Number 8
July and August 2013Pages 851-857
- Receive Date 17 August 2014
| Article View | 20,384 |
| PDF Download | 7,169 |