Document Type : Original Article

Author

Young researchers club, Boroujed branch, Islamic Azad University, Boroujerd, Iran

Abstract

A field experiment was performed in order to evaluate the effects of irrigation and application of K fertilizer on seed storage proteins  in chickpea, ILC-482 cultivar. Experiment was performed in factorial using randomized complete block design with three replications. In this experiment, two factor consist of irrigation in three levels (I1= non irrigation and I2= irrigation in flowering stage), K fertilizer in six levels (K1=0, K2=25kg/ha, K3=50kg/ha, K4=75kg/ha, K5=100kg/ha and K6=125kg/ha) was studied. The results showed that the effect of irrigation on seed storage proteins and protein yield was significant. Also results showed that effect of K fertilizer on seed storage proteins and protein yield was not significant. In non irrigation treatment seed storage proteins was increased compared to irrigation treatment and protein yield decreased. Also, results showed that no effects treatments (irrigation and K fertilizer) on protein banding patterns. Also, results indicated that not obvious any new band and not deleted any bands in all treatments

Keywords

Azimi, S.M., Farnia, A., Shaban, M. and Lak, M. (2013). Effect of different biofertilizers on Seed yield of barley (Hurdeom vulgar L.), Bahman cultivar. International journal of Advanced Biological and Biomedical Research. Volume 1, Issue 5: 538-546.
Beyranvand, H., Farnia, A., Nakhjavan, SH. and Shaban, M. 2013. Response of yield and yield components of maize (Zea mayz L.) to different bio fertilizers. International journal of Advanced Biological and Biomedical Research. Volume 1, Issue 9: 1068-1077.
Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities ofprotein utilizing the principle of protein-dye binding. Ann. Biochem., 72: 248-254.
Cereletti, P. 1979. The Legume Proteins. Proc. Congr. PPI. 30 May -2 June, Perugia, Italy. pp. 31-57.
Dupont FM, Altenbach SB (2003) Molecular and biochemical impacts of environmental factors on wheat grain development and protein synthesis. J Cereal Sci 38:133–146.
Iqbal, S.H., A. Ghafoor and N. Ayub. (2005). Relationship between SDS-PAGE markers and Ascochyta blight in chickpea. Pak. J. Bot., 37: 87-96.
FAO, 2003. Production Year Book, 2002. Food and Agriculture Organization of the United Nations (FAO), Rome, Italy. http://apps.fao.org.
Isemura T., Shiyo N., Shigeyuki M., et al. (2001) Genetic variation and geographical distribution of Azuki bean (Vigna angularis) landraces based on the electrophoregram of seed storage proteins. Breeding Sci., 51: 225-230.
Javid, A., A. Ghafoor and R. Anwar. (2004). Seed storage protein electrophoresis in groundnut for evaluating genetic diversity. Pak. J. Bot., 36: 87-96.
Karim, M.F. and Q.A. Fattah, (2006). Changes inyield of chickpea biocomponents of chickpea (Cicer arietinum L.)sprayed with potassium napthenate and napthenicacetic acid. Bangladesh J. Bot., 35(1): 39-43.
Kim CS, Kamiya S, Sato T, Utsumi S, Kito M (1990). Improvement of nutritional value and functional properties of soybean glycinin by protein engineering. Protein Eng. 3: 725-731.
Laemmli, U.K. (1970). Cleavage of structure proteins assembly of the head of bacteriophage T4.Nature, 22: 680-685.
Luo C, Branlard G, Griffin WB, McNeil DL (2000) The effect of nitrogen and sulphur fertilization and their interaction with genotype on wheat glutenins and quality parameters. J Cereal Sci 31: 185–194.
Osborne, T. B.,(1924) The Vegetable Proteins, Longmans Green, London, 1924.
Ottman MJ, Doerge TA, Martin E (2000) Durum grain quality as affected by nitrogen fertilization near anthesis and irrigation during grain fill. Agron J 92: 1035–1041.
Prihar SS, Sandhu BS (1968) Irrigation of field crops, Indian Council of Agric. Res New Delhi 142.
Rharrabti Y, Villegas D, Garcia Del Moral LF, Aparicio N, Elhani S, Royo C (2001) Environmental and genetic determination of protein content and grain yield in durum wheat under Mediterranean conditions. Plant Breed 120:381–388.
SAS Institute (2002) JMP statistics and graphics guide. SAS Institute Inc., Cary, NC. Singh, A.K., R.K. Chaudhry and R.P.R. Shaema, (1993). Effect of inoculation and fertilizer level on yield, nutrients uptake and economics of summer pulses. Ind. J. Potassium Res., 9: 176-178.
Singh KB, Malhotra RS, Halila MH, Knights EJ, Verma MM (1994) Current Status and Future Strategy in Breeding Chickpea for Resistance to Biotic and Abiotic Stresses, in Expending the Production and Use of Cool Season Food Legumes, Eds. F.J. Muehlbauer and W.J. Kaiser, Klwer Academic Pub., printed the Netherlands, p:572-591.
Shaban, M. (2013a). Application of seed equilibrium moisture curves in agro physics. International journal of Advanced Biological and Biomedical Research. Volume 1, Issue 9: 885-898.
Shaban, M. (2013b). Biochemical aspects of protein changes in seed physiology and germination.
International journal of Advanced Biological and Biomedical Research. Volume 1, Issue 8: 885-898.
Sumera Iqbal and Asghari Bano.(2009). Water stress induced changes in antioxidant enzymes, membrane stability and seed protein profile of different wheat accessions. African Journal of Biotechnology Vol. 8(23), pp. 6576-6587.
Suoyi Han, Rui Fan, Tuanjie Zhao, Jingjing Yu and Deyue Yu.( 2009). Seed storage protein components are associated with curled cotyledon phenotype in soybean. African Journal of Biotechnology Vol. 8 (22),pp. 6063-6067.
Tanksley S.D., Jones R.A. (1981) Application of alcohol dehydrogenase allozymes in testing the genetic purity of F1 hybrids of tomato. Hort. Sci., 16: 179-181.
Tea I, Genter T, Naulet N, Boyer V, Lummerzheim M, Kleiber D (2004) Effect of foliar sulfur and nitrogen fertilization on wheat storage protein composition and dough mixing properties. Cereal Chem 81: 759–766.
Thanh V.O.C., Hirata Y. (2002) Seed storage protein diversity of three rice species in the Mekong Delta. Biosphere Conservation, 4: 59-67.