International Journal of Advanced Biological and Biomedical Research

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Determination of Herpetic Keratitis Frequency Using Loop Mediated Isothermal Amplification (LAMP) Method in Isfahan, Iran

Document Type : Original Article

Authors

Maryam Tarighati 1
Vajihe Karbasizade 2
Sharareh Moghim 3

1 MSc student: Department of Microbiology, School of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

2 PhD, Assistant Professor, Department of Microbiology, School of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

3 PhD, Assistant Professor, Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract
Herpetic keratitis which is resulted from herpes simplex virus type I, is considered the most common cause of corneal blindness that needs being diagnosed and treated in order to prevent irreversible eye effects. The best method to diagnose it is molecular method which has high sensitivity and exclusivity. The current study was done on 25 eye samples collected from patients referred to the Feyz hospital of Isfahan who were diagnosed with herpetic keratitis disease. After cornea sampling and extracting DNA from eye samples, LAMP-PCR technique was done to search genome of herpes simplex virus type I. Among 25 suspected cases of herpetic keratitis, 5 samples were confirmed to have genome virus of HSV1 and herpetic keratitis frequency was determined by 20%. The results of this investigation showed a relative high frequency of herpetic keratitis in this zone using LAMP-PCR diagnostic method compared with similar studies which indicates high sensitivity of this technique. Therefore, using such rapid, accurate and cheap molecular method is suggested

Keywords

Herpes simplex virus type I
Herpetic keratitis
LAMP-PCR

Subjects

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International Journal of Advanced Biological and Biomedical Research
Volume 1, Issue 11 - Serial Number 11
November and December 2013
Pages 1431-1435

  • Receive Date 20 August 2014

Article View 41,088
PDF Download 10,760

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