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Characterization of Mutations in the Rpob and Katg Gene of Mycobacterium Tuberculosis Isolates From Pasteur Institute of Tehran
Authors
1 Young Researchers and Elites Club, North Tehran Branch, Islamic Azad University, Tehran, Iran
2 Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran
3 Biotechnology Research Center, venom & Biotherapeutics Molecules Lab., Pasteur Institute of Iran, Tehran, Iran
Abstract
Objective: The Rifampicin resistance and susceptibility of Mycobacterium tuberculosis are caused by mutations in the 81-base pair region of the rpoB gene encoding the b-subunit of RNA polymerase. Methods: Isoniazid resistance of M. tuberculosis is related to mutations in inha , oxyR and ahpC genes which 30 to 90 percent of Isoniazid resistance is occurred in 3015 codons of katg gene. The rpoB and katG sequences of 30 isolates were analyzed to identify the mutations and compare the mutations with their related susceptibilities. Results: In this research, we investigated the location and type of rpoB and katG mutations in Mycobacterium tuberculosis which had been achieved from Pasteur Institute of Tehran. PCR Amplification and DNA sequencing methods were performed. In this assay, from 507 to 537 codons and 315 codons of rpoB and katG genes were sequenced and also mutations were analyzed, respectively.
Keywords
Subjects
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| Characterization of rpomutations in rifampin | |||||
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for Treating Pulmonary Tuberculosis (Review). Ltd, The Cochrane Collaboration.
Feuerriegel, S., Oberhauser, B., George, A.G., Dafae, F., Richter, E., R¸sch-Gerdes, S.,Niemann, S. (2012). Sequence analysis for detection of first-line drug resistance in Mycobacterium tuberculosis strains from a high-incidence setting. BMC Microbiology, 90(12): 1-10.
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Taiwan. JMII, 46(4): 266-270.
Pym, A.S., Saint-Joanis, B.,Cole, S.T. (2002). Effect of katG mutations on the virulence of Mycobacterium tuberculosis and the implication for transmission in humans. Infect Immun, 70: 4955-4960.
Simon, A. W., Stuart, M. W., Malcolm, D. Y., Francis, A. D. (1998). Comparison of Three Molecular Assays for Rapid Detection of Rifampin Resistance in
Spies, F.S., von Groll, A., Ribeiro, A.W., Ramos, D.F., Ribeiro, M.O., Dalla Costa, E.R., Martin, A., Palomino, J.C., Rossetti, M.L., Zaha, A.,da Silva, P.E.A. (2013). Biological cost in Mycobacterium tuberculosis with mutations in the rpsL, rrs, rpoB, and katG genes. Tuberculosis, 93(2): 150-154.
Van Soolingen, D. (2001). Molecular epidemiology of tuberculosis and other mycobacterial infections: main methodologies and achievements. J Intern
Med, 249: 1-26.
Volume 2, Issue 8 - Serial Number 8
July and August 2014Pages 2161-2165
- Receive Date 13 September 2014
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