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Effects of the Methanolic Extract of Abeloschus Esculentus (L) Moench (Okro) Fruit oOn the Testes and Sperm Characteristics of Male Albino Wistar Rats
Document Type : Original Article
Authors
Department of human physiology Faculty of Basic Medical Sciences, College of Health sciences, University of Port Harcourt, Port Harcourt Nigeria
Abstract
Objective: In this study aimed at evaluating the antifertility effect of the methanolic extract of Abelmoschus esculentus (okro) fruit using albino wistar rat. Methods: Fifty adult male rats weighing 200g to 300g were weighed and divided into five groups of five rats each. Group1 served as the control and were given water and normal rat chaw while groups 2, 3, 4 and 5 served as the test groups and were orally administered with (250, 500, 750 and 1000)mg/kg of okro extract respectively after three weeks of acclimatization. Following six weeks of extract administration, the rats were reweighed and sacrificed. Semen was collected from their caudal epididymis for sperm analysis and their testes were weighed and processed for histological studies. Results: Results from the test groups showed a significant (p≤0.05) reduction in the testicular weight and a significant (p≤0.05) increase in the body weight of the rats when compared with the control group. There was also a significant (p≤0.05) decrease in sperm count and sperm motility with a corresponding increase in the percentage number of abnormal sperm cells The histology of the testes showed degenerating testicular tissues. From the result, we therefore conclude that consistent consumption of okro may be destructive to the testes and as such, could impair male fertility.
Keywords
Subjects
Brugh VM, Lipshultz LI (2004). "Male factor infertility". Medical Clinics of North America 88 (2): 367ñ85.
Canadian Council of Animal Care (CCAC), (1985). Guide to the handling and use of experimental animals. Ottawa: ont; 2 United States NIH publications, No. 85-230:45-47.
Dan R.D. and Gu C, (2010). Inhibition Effect of Okra Polysaccharides on Proliferation of Human Cancer Cell Lines. Journal Food science, 21.
Deshpande V.Y, Mendulkar K.N, Sadre N.L (1980). "Male antifertility activity of Azadirachta Indica in mice". Journal of Postgraduate Medicine 26 (3): 167ñ70.
Dioscorides (ca. 40 A.D.). De Materia Medica. (translated by Goodyer (1655), modified and published 1933 by Robert Gunther).
El-Malak G.A.A., (2007). Evaluation of free radical scavenging by natural antioxidants in some fresh, blanched, cold and frozen storage of vegetables. Annals of Agricultural Science (Cairo), 52(2): 367-374.
Hadley MA, Lin YC, Dym M. (1981). "Effects of gossypol on the reproductive system of male rats". J Androl. (2): 190ñ9.
Kahlon T.S, Chiu M.C.M, Chapman M.H (2007b). Steam cooking significantly improves in vitro bile acid binding of beets, eggplant, asparagus, carrots, green beans, and cauliflower. Nutrition Research, 27(12): 750-755.
Lengsfeld C, Titgemeyer F, Faller G, Hensel A (2004). Glycosylated compounds from okra inhibit adhesion of Helicobacter pylori to human gastric mucosa. Journal of Agricultural and Food Chemistry, 52(6): 1495-1503.
Lohiya NK, Manivannan B, Mishra PK et al. (2002). "Chloroform extract of Carica papaya seeds induces longterm reversible azoospermia in langur monkey". Asian J Androl. 4 (1): 17ñ26.
Olatunji-Bello I.I., Ijiwole, T. FAwobajo.O.(2009). Evaluation of the Deleterious Effects of Aqueous Fruit Extract of Abelmoschus Esculentus (Okro Fruit) on Some Male Reproductive Parameters in Sprague Dawley Rats; Journal of Phytology, Vol 1, No 6
Sabitha V., Ramachandra S., Naveen K.R., and Panneerselvam (2011). Antidiabetc an esculentus(L) Moench. in streptozotocin-induced
diabetic rats. Journal of pharmacy and bioallied sciences 3(3):397-402.
Savello P.A., Martin F.W. and Hill J.M (1980). Nutritional composition of okra seed meal, Journal of Agric food chemistry, 28(6): 1163-1166.
Sekiya K (1997). Inhibition of cyclooxygenase and lipoxygenase by foods. Bulletin of the Shikoku National Agricultural Experiment Station, No. 61: 31-37.
Sinclair S., (2000). Male infertility: nutritional and environmental consideration: alternative medicine review 5: 28-38.
Vayssade M., Sengkhamparn N., Verhof R., Delaigue C., Oundiam O., Vingneron P., Voragen A.G.J., Schols H.A. and Nagel M.D, (2010). Antiproliferative and Proapoptotic Actions of Okra Pectin on B16F10 Melanoma Cells, Phytotherapy Research journal, 24(7): 982-989.
Venma P.K, Sharma A, Mathur A et al. (2002). "Effect of Sarcostemma acidum stems extract on spermatogenesis in male albino rats". Asian Journal of Andrology 4 (1): 43ñ7.
Canadian Council of Animal Care (CCAC), (1985). Guide to the handling and use of experimental animals. Ottawa: ont; 2 United States NIH publications, No. 85-230:45-47.
Dan R.D. and Gu C, (2010). Inhibition Effect of Okra Polysaccharides on Proliferation of Human Cancer Cell Lines. Journal Food science, 21.
Deshpande V.Y, Mendulkar K.N, Sadre N.L (1980). "Male antifertility activity of Azadirachta Indica in mice". Journal of Postgraduate Medicine 26 (3): 167ñ70.
Dioscorides (ca. 40 A.D.). De Materia Medica. (translated by Goodyer (1655), modified and published 1933 by Robert Gunther).
El-Malak G.A.A., (2007). Evaluation of free radical scavenging by natural antioxidants in some fresh, blanched, cold and frozen storage of vegetables. Annals of Agricultural Science (Cairo), 52(2): 367-374.
Hadley MA, Lin YC, Dym M. (1981). "Effects of gossypol on the reproductive system of male rats". J Androl. (2): 190ñ9.
Kahlon T.S, Chiu M.C.M, Chapman M.H (2007b). Steam cooking significantly improves in vitro bile acid binding of beets, eggplant, asparagus, carrots, green beans, and cauliflower. Nutrition Research, 27(12): 750-755.
Lengsfeld C, Titgemeyer F, Faller G, Hensel A (2004). Glycosylated compounds from okra inhibit adhesion of Helicobacter pylori to human gastric mucosa. Journal of Agricultural and Food Chemistry, 52(6): 1495-1503.
Lohiya NK, Manivannan B, Mishra PK et al. (2002). "Chloroform extract of Carica papaya seeds induces longterm reversible azoospermia in langur monkey". Asian J Androl. 4 (1): 17ñ26.
Olatunji-Bello I.I., Ijiwole, T. FAwobajo.O.(2009). Evaluation of the Deleterious Effects of Aqueous Fruit Extract of Abelmoschus Esculentus (Okro Fruit) on Some Male Reproductive Parameters in Sprague Dawley Rats; Journal of Phytology, Vol 1, No 6
Sabitha V., Ramachandra S., Naveen K.R., and Panneerselvam (2011). Antidiabetc an esculentus(L) Moench. in streptozotocin-induced
diabetic rats. Journal of pharmacy and bioallied sciences 3(3):397-402.
Savello P.A., Martin F.W. and Hill J.M (1980). Nutritional composition of okra seed meal, Journal of Agric food chemistry, 28(6): 1163-1166.
Sekiya K (1997). Inhibition of cyclooxygenase and lipoxygenase by foods. Bulletin of the Shikoku National Agricultural Experiment Station, No. 61: 31-37.
Sinclair S., (2000). Male infertility: nutritional and environmental consideration: alternative medicine review 5: 28-38.
Vayssade M., Sengkhamparn N., Verhof R., Delaigue C., Oundiam O., Vingneron P., Voragen A.G.J., Schols H.A. and Nagel M.D, (2010). Antiproliferative and Proapoptotic Actions of Okra Pectin on B16F10 Melanoma Cells, Phytotherapy Research journal, 24(7): 982-989.
Venma P.K, Sharma A, Mathur A et al. (2002). "Effect of Sarcostemma acidum stems extract on spermatogenesis in male albino rats". Asian Journal of Andrology 4 (1): 43ñ7.
Volume 2, Issue 10 - Serial Number 10
September and October 2014Pages 2686-2690
- Receive Date 09 November 2014
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