Document Type : Original Article

Authors

1 Department of Pharmacy, The University of Lahore, Lahore, Pakistan

2 College of Pharmacy, University of the Punjab, Lahore, Pakistan

10.22034/ijabbr.2021.523871.1344

Abstract

Introduction: Periploca aphylla is belongs to family Asclepiadaceae and it is an important traditional plant which has been utilized for decades for its local uses.
Methods: This research work included pharmacognostic studies, microscopic evaluation, phytochemical and physiochemical tests for the effective identification and characterization of plant. Transverse section of plant shows the arrangement of cells. Fluorescence analysis shows colors in light of different wavelength. Fluorescence analysis was performed with various reagents and chemicals to observe characteristic pattern and colors for the identification and proper authencity of plant and it was reported in this paper.
Results: Phytochemical studies show the presence of alkaloids, saponins, flavonoids and tannins. Powder study shows lignified fibers, spiral vessels and lignified tracheid. In this study Fourier-transform infrared spectroscopy was used for the identification of plant and for the effective recognition of biological composition of plant. The IR Spectra were performed and reported in this paper. All these parameters are necessary for the characterization and identification of plant and a new doorstep to find out any adulteration in the plant.
Conclusion: Anti-oxidant in-vitro studies were performed for the evaluation of any anti-oxidant property which provided a brief description about its pharmacological effects regarding rancidity and also a description about its further usage in any of anti-oxidant formulation.

Graphical Abstract

Pharmacognostic Features, Preliminary Phytochemical Screening and in Vitro Antioxidant Studies of Indigenous Plant of Lahore

Keywords

Main Subjects

1. Sharma S, Gupta V. (2008). In vitro antioxidant studies of Ficus racemosa Linn. root. Pharmacognosy Magazine, 4(13): 70.
2. Fazeli-Nasab B. (2018). The effect of explant, BAP and 2, 4-D on callus induction of Trachyspermum ammi. Potravinarstvo, 12(1): 578–586. https://doi.org/10.5219/953
3. Sobhanizadeh A, Solouki M, Fazeli-Nasab B. (2017). Optimization of callus induction and effects of biological and non-biological elicitors on content of phenol/flavonoid compounds in Nigella sativa under in-vitro conditions. Journal of Cell & Tissue, 8(2): 165-184. https://doi.org/10.29252/JCT.8.2.165
4. Farnsworth N R, Soejarto D D. (1991). Global importance of medicinal plants. The conservation of medicinal plants, 26: 25-51.
5. Mahmood A, Mahmood A, Naveed I, Memon M M, Bux H, Majeed M Y, Mujtaba G, Mumtaz M S. (2011). Indigenous medicinal knowledge of common plants used by local people of Hattian Bala District, Azad Jammu and Kashmir (AJK), Pakistan. Journal of Medicinal Plants Research, 5(23): 5517-5521.
6. Qureshi R. (2012). Medicinal flora of hingol national park, Baluchistan, Pakistan. Pak. J. Bot, 44(2): 725-732.
7. Alam N, Shinwari Z, Ilyas M, Ullah Z. (2011). Indigenous knowledge of medicinal plants of Chagharzai valley, District Buner, Pakistan. Pak J Bot, 43(2): 773-780.
8. Ahmad I, Ibrar M, Ali N. (2011). Ethnobotanical study of tehsil kabal, swat district, KPK, pakistan. Journal of Botany, 2011.
9. Gami B, Parabia M. (2010). Pharmacognostic evaluation of bark and seeds of Mimusops elengi L. Int J Pharm Pharm Sci, 2(Suppl 4): 110-113.
10. Ranjith D. (2018). Fluorescence analysis and extractive values of herbal formulations used for wound healing activity in animals. Journal of Medicinal Plants Studies, 6(2): 189-192.
11. Dodoo K, Kitcher C, Gordon A, Frimpong-Manso S, Schwinger G. (2020). Pharmacognostic characteristics and mutagenic studies of Alstonia boonei De Wild. Research Journal of Pharmacognosy, 7(1): 7-15.
12. Peachey L D. (1958). Thin sections: I. A study of section thickness and physical distortion produced during microtomy. The Journal of Cell Biology, 4(3): 233-242.
13. Satir P G, Peachey L D. (1958). Thin Sections: II. A Simple Method for Reducing Compression Artifacts. The Journal of Cell Biology, 4(3): 345-348.
14. Zelander T, Ekholm R. (1960). Determination of the thickness of electron microscopy sections. Journal of ultrastructure research, 4(3-4): 413-419.