Document Type : Original Article

Authors

1 Department of Reclamation of Arid and Mountainous Region, Natural Resources Factually, the University of Tehran, Karaj, Iran

2 Agronomy and Plant Breeding Department (APBD), College of Agriculture and Natural Resources, the University of Tehran, Karaj, Iran

Abstract

Medicinal use of the opium poppy (Papaver somniferum L) has ancient history, but the isolation of morphine was not described until the early nineteenth century. Morphine is the most important alkaloid of opium poppy in the last 50 years. In the morphine pathway has been reported to generate morphine in this species, CODM has a crucial role as the gene coding the enzyme responsible for demethylation of codeine to morphine. In this study we extract of CODM gene sequence from Papaver fogax and Papaver oriental. The sequence of this gene via in silico technique converted to the protein sequence.  After this step we use the bioinformatics tools for analysis of these sequences.

Keywords

[1] Özcan MM, Atalay C. Determination of seed and oil properties of some poppy (Papaver somniferum L.) varieties. Grasasy Aceite, 2006, 57, 169–174.
[2] Attwood TK, Parry-Smith DJ. In Introduction to Bioinformatics. Ed Wood (ed.). Pearson Education Asia ltd, India. 2005, 5(17):3-20
[3] Zhu JK. Salt and drought stress signal transduction in plants. Annu Rev Plant Biol. 2002, 53, 247–273.
[4] Lloyd G, Landini P, Bushby S. Activation and repression of transcription initiation in bacteria. Essays Biochem. 2001,37, 17–31
[5] Hagel JM, Facchini, PJ. Dioxygenases catalyze the O demethylation steps of morphine biosynthesis in opium poppy. Nature Chemical Biology, 2010, 273-275.
[6] Mu˜noz-Ledesma FJ. Etiologíay control de la necrosis de la adormidera Papaver somniferum L. PhD thesis, Universidad de Cordoba, 2003, 340 pp (in Spanish with English summary).
[7] Kendrew JC, Dickerson RE, Strandberg BE, and Hart RG, Davies DR, Phillips DC and Shore VC. Structure of myoglobin: a three-dimensional Fourier synthesis at 2Å resolution. Nature, 1960, 185 (4711): 422–427