Document Type : Original Article


1 Infectious Diseasec and Tropical Medicin Research Center Zahedan University of Medical Scienes, Zahedan, Iran

2 Department of Microbiology, Medical faculty, Kerman university of medical science, Kerman, Iran

3 Department of Microbiology, Kerman Science and Research Branch, Islamic Azad University, Kerman, Iran


The purpose of this study was to examine the evolution of antimicrobial activity of flower extract of Hibiscus sabdariffal against antibiotic-resistant Escherichia coli and Staphylococcus aureus that isolates from the urinary tract infection by microtiterplate method. All 42 strains 30 E.coli and 12 S. aureus isolated from urine culture of hospitalized patients in Zabol ( Zabol, south-eastern Iran) suffered from urinary tract infections during the years 2011- 2012 were evaluated and the extract of Hibiscus sabdariffalobtained by rotary and the minimum inhibitory concentrations were investigated to characterize the antimicrobial activities of this extract. The result show that E. coli were resistance to 4 of the agent and more resistance to tetracyclin(63.3%), erythromycin(56.6%) and cefixime(40 %), S.aureus  more sensitive were antibiotic resistance to vancomycin and cefixime. The highest MIC values was found to be 20mg/ml against two E.coli.  The leas MIC values was found to 1.25 mg/ml against three S.aureus.


Almeida CF, Amorim ELC, Albuquerque UP and Maia MB.2006. Medicinal plants popularly used in the Xingo region – a semi – arid location in Northeastern Brazil. J. Arid
Environ. 63: 109-114.
Aletor VA .1993. Allelochemicals in plant food and feedstuffs:in nutritional biochemistry and physiopathological aspects in animal production.Veterinary and Human Toxicology. 35(1):57- 67.
Akindahunsi AA, Olaleye MT. 2003.Toxicological investigation of aqueous methanolic extract of Hibscus sabdariffa L. J Ethnopharmacol. 89:161–164
Akond MA, Alam S, Hassan MR, Shirin M. 2009.Antibiotic Resistance of Escherichia coli Isolated From Poultry and Poultry Environment of Bangladesh. Internet Journal of food safety. 11: 19-23.
Al-Hashimi A G. 2006.Antioxidant and antibacterial activities of  Hibiscus sabdariffa L. extracts.   African Journal of Food Science. 6(21) :506-511,
Builders PF, Ezeobi CR, Tarfa FD, Builders MI. 2010. Assessment of the intrinsic and stability properties of the freeze-dried and formulated extract of Hibiscus sabdariffa (Linn). Afr J Pharm Pharmacol. 4:304-13.
Chen CC, Hsu JD, Wang SF, Chrang HC, Yang MY, Kao ES, Ho YO, Wang CJ. 2003.Hibscus sabdariffa extract inhibit the development of atherosclerosis in cholesterol-fed rabbits. J Agric Food Chem. 51:5472–5477. [PubMed]
Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing (2002), 16th International supplement. CLSI document M100-S12
Davis, H.R (1997)
Dalziel JM.1973. The Useful Plants of West Tropical Africa. The Crown Agents; London: 314–315.
Dien A. Limyati; Lisa Soegianto. 2008.Activitas antibakteri ekstrak kelopak rosela (Hibiscus sabdariffa L.) terhadap Staphylococcus aureus dan Streptococcus pyogenes Indonesian Scientific Journal Database. 7(1):47-53.
Evans SA, Brachman SP. 1991. Bacterial Infections of Humans: Staphylococcal Infections, Company New York and London: Plenum Medical Book.
Fasoyiro SB, Ashaye OA, Adeola A, Samuel FO. 2005. Chemical and storability of fruit-flavoured (Hibiscus sabdariffa) drinks. World J Agric Sci. 1:165-8.
Forbes BA, Sahm DF, Weissfeld AS. 2007.Bailey & Scott`s diagnostic microbiology. 12th ed. Missouri: Mosby Co. 323-333.
Fullerton M, Khatiwada J,  Johnson JU, Davis S and Williams LL. 2011. Determination of Antimicrobial Activity of Sorrel (Hibiscus sabdariffa) on Esherichia coli O157:H7 Isolated from Food, Veterinary, and Clinical Samples. J Med Food. 14(9): 950–956.
Jung E, Kim Y, Joo N.2013. Physicochemical properties and antimicrobial activity of Roselle (Hibiscus sabdariffa L.). J Sci Food Agric.
Jonadet M, Bastide J, Bastide P, Boyer B, Carnat AP. 1990. Lamaison JL. In vitro enzyme inhibitory and in vivo cardio-protective activities of Hibiscus sabdariffa L. J Pharmacol Belgium. 45:120–124. [PubMed]
Monteiro JM, Albuquerque UP, Lins-Neto EMF, AraujoEL and Amorim ELC.2006. Use patterns and knowledge of medicinal species among the two rural communities in Brazil’s semi-arid northeastern region.J. Ethnopharmacol. 105: 173-186.
Nwachukwu MI, Uwaezuke JC, Nwachukwu IO, Ukaga CN and Anyanwu VE. 2009.Phytochemical analysis and antimicrobial activities of extracts of calyces of Hibiscus sabdariffa var. altissima on Escherichia coli and Staphylococcus aureus. Nigerian Journal of Microbiology. 23(1);1896 – 1900
Nwaiwu NE, Mshelia F and Raufu IA. 2012.Antimicrobial Activities of Crude Extracts of Moringa oleifera, Hibiscus sabdariffa and Hibiscus esculentus Seeds Against Some Enterobacteria.
Olaleye T. 2007. Cytotoxicity and antibacterial activity of methanolic extract of Hibiscus sabdariffa. J. Med. Plants Res. 1(1):9-13.
Soltani R, Khalili H, Rasoolinejad M, Gholami K, Abdollahi A. 2012. Antimicrobial susceptibility pattern of Staphylococcus aureus strains isolated from hospitalized patients in Tehran, Iran Research in Pharmaceutical Sciences. 7(5).
Tricia DM, McLaughlin W, Brown PD. 2006.Antimicrobial resistance of Escherichia coli isolates from broiler chickens and humans. BMC. Vet. Res. 2006; 2: 7.
Wang CJ, Wang JM, Lin WL, Chu CY, Chou FP, Tseng TH. 2000. Protective effect of Hibiscus anthocyanins against tert-butyl hydroperoxide–induced hepatic toxicity in rats. Food Chem Toxicol. 38:411–416. [PubMed]