International Journal of Advanced Biological and Biomedical Research

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37
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Cellulase Enzymes Production from Pistachio Hull by Different Strains of Tichoderma Fungi

Document Type : Original Article

Authors

A. Mohamadi 1
S. Shahbazi 2

1 Biotechnology Department, Faculty of Agriculture, Pyam e Noor University, Alborz, Iran

2 Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute (NSTRI), Atomic Energy Organization of Iran (AEOI), Karaj, Iran

Abstract
Six strain of Trichoderma (T. viride, T. harzianum, T. reesei, T. longibrachiatum, T. koningii, T. virens) were isolated and used for extracellular enzyme production. Species of Trichoderma are collected from the surrounding soil of suger beet root fields in East Iran, and held in vitro cultured PDA. TCM and TFM are used for the growth of spores and induceing the production of Cellulase. Finally the enzyme is measured at 50ºC for 60 minutes by DNS method and the samples are absorbed by spectrophotometry at wavelength 540 nm. By product of Pistachio was used to enzyme production and extracellular protein production and endo-glucanase, exoglucanase, β-glucosidase and FPase activity was investigated. T. harzianum produces high levels of endo-glucanase, exoglucanase and total cellulase, which can be further improved by controlled culture conditions. This strain can be a good candidate for obtaining cellulases from lignocellulosic by products of pistachio hull.

Keywords

Trichoderma spp
Pistachio hull
Cellulase
Enzyme Activity
International Journal of Advanced Biological and Biomedical Research
Volume 2, Issue 4 - Serial Number 4
March and April 2014
Pages 1051-1059

  • Receive Date 28 July 2014

Article View 19,964
PDF Download 6,661

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