International Journal of Advanced Biological and Biomedical Research

MSRT,ISC
37
h-index

Evaluation of Antioxidant Activity of Methanolic Extract of Elaegnus Angustifolia by Frap Assay

Document Type : Original Article

Authors

Maryam Sardarodiyan 1
Akram Arianfar 2
Masoomeh Mehraban Sang Atash 3

1 Young Researchers and Elite Club, Quchan Branch, Islamic Azad University, Quchan, Iran

2 Department of Food Science and Technology, Quchan Branch, Islamic Azad University, Quchan, Iran

3 Food Science and Technology Research Institute, ACECR Mashhad Branch, Mashhad, Iran

Abstract
Nowadays the researches have indicated the fact that using synthetic antioxidants as food additives in edible oils can have adverse effects on human health. So preparing antioxidation resources for reduction of oxidation stress effects is important. The aim of this project was, representing a natural antioxidant source with frap test. The present study optimized the ultrasound assisted extraction (UAE) conditions to maximize the antioxidant activity [Ferric ion Reducing Antioxidant Power (FRAP)] of extracts from Elaegnus angustifolia. Optimizing the process three factor (time, temperature and solution to solid ratio) were investigated each factor was repeated three times (5, 20, 35 min), temperature (30, 45, 60), and solution to solid ratio (20, 35, 50). The experiment was conducted based on Box-Behnken design with three factor and three levels comprising a total of 17 tests. During optimization tests in an experiment with extraction conditions as (time: 26.10 min, temperature: 44.68 and solution to solid ratio: 29.56) the highest antioxidant compound 36.89 mol Fe2+/g of extract was recorded. TheElaegnus angustifoliaextract possessed a relatively high antioxidant activity and might be considered as a rich source of natural antioxidant.
 

Keywords

Antioxidant
Elaegnus angustifolia
Extract optimizing
Frap
Ultrasound
International Journal of Advanced Biological and Biomedical Research
Volume 2, Issue 4 (2)
March and April 2014
Pages 381-390

  • Receive Date 19 October 2014

Article View 19,147
PDF Download 6,053

Home

A-Z Journals

SPC Publisher's Policies

Indexing and Abstracting

Editorial Board

Guide for Authors

EndNote file for authors

Submit Manuscript

Contact Us