International Journal of Advanced Biological and Biomedical Research

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37
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Antimicrobial ‌in Vitro and in Vivo Potential of Five Lichen Species on Fusarium Equiseti and Pectobacterium Carotovora Pv. Carotovora Causal Agents of Potato Rots

Document Type : Original Article

Authors

Farid Houshyar 1
Soleiman Jamshidi 2
Mohammad Sohrabi 3

1 Former master student of plant pathology, Department of Agriculture Management, College of Agriculture, Malekan Branch, Islamic Azad University, Malekan, Iran

2 Assistant professor of plant pathology, Plant Protection Department, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran

3 Assistant professor of Biotechnology Research Center, Irananian Research Organization for Science and Technology,Iran

Abstract
Potato is one of the main crops which is suffering from the great losses in storage and the most conventional method of its tuber rot is using hazardous chemicals. Using antimicrobial potential of lichens can be one of the safe, green, environment friendly methods for plant diseases management. In this study, antimicrobial activity of acetone, methanol, chloroform extracts of Ramalina capitaat, Xanthoparmelia stenophylla, Umbilicaria cylindrical, Rhizoplaca crysoleuca and Anamylopsora pulcherrima were evaluated against two potato rot agents vic. Fusarium equiseti and Pectobacterium carotovora pv. carotovora in laboratory and storage conditions. Bioassay tests were disc diffusion and minimum inhibitory and bactericide concentration methods. Dimethyl sulfoxide solvent on paper disc was used as negative control. Positive controls were considered as %0.2 benomyl and gentamicin antibiogram discs for fungus and bacterium, respectively. There were no effect of lichens extract on fungus. R. chrysoleuca especially methanol and chloroform extracts of had remarkable bactericide and bacteriostatic effect on bacterium. The study with selected extracts in storage condition showed preventive effect of 80% of extracts on bacterium. The most protective effect was observed in methanol extract of R. chrysoleuca. Therefore, lichen extract would be promising biological product would be a potential replacement instead of chemicals.

Keywords

Antimicrobial effect
MIC
MBC
Natural product
Potato rot
International Journal of Advanced Biological and Biomedical Research
Volume 2, Issue 4 (2)
March and April 2014
Pages 412-417

  • Receive Date 19 October 2014

Article View 19,519
PDF Download 6,231

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