International Journal of Advanced Biological and Biomedical Research

The Correlation of Some Secondary Metabolites of Alfalfa (Medicago sativa L.) with Plant Organ and Harvest Time

Hamid Reza Vahidipour; Monireh Cheniany; Mehrdad Lahouti; Ali Ganjeali; Maryam Moghaddam Matin

Volume 12, Issue 1 , January 2024, , Pages 28-43

https://doi.org/10.48309/ijabbr.2024.2013056.1464

Abstract

Background: Alfalfa (Medicago sativa L.) is an important source of phytoestrogens. The abundance of alkaloids, phenols, flavonoids, and isoflavonoids has made this plant a rich source ... Read More Background: Alfalfa (Medicago sativa L.) is an important source of phytoestrogens. The abundance of alkaloids, phenols, flavonoids, and isoflavonoids has made this plant a rich source of these plant estrogens. Cultivation of alfalfa as a rich source of phytoestrogens for medicinal purposes has provided opportunities for alternative use of this forage.Methods: The study was carried out as a hydroponic culture in a perlite-cocopeat compacted bed with three replications. Roots and shoots of alfalfa plants were sampled separately in two stages (the 30th and 60th days after sowing). Plant samples were extracted with methanol solvent, and total phenols, flavonoids, and isoflavonoids contents were measured by spectrophotometric colorimetric method.Results: The data analysis showed a significant effect of plant organ and harvest time on the contents of total phenols, flavonoids, and isoflavonoids (P≤0.05). The maximum accumulation of these compounds was in the plant shoots, and with the increase of the harvest time, the content of these phytoestrogens increased. Spearman's correlation analysis showed the different effects of the plant organ on the correlation level of the mentioned metabolites, so the flavonoids of the roots and shoots showed the most positive correlation, while isoflavonoids did not show a significant correlation (P≤0.05).Conclusions: The presence of the maximum contents of total phenols, flavonoids, and isoflavonoids in the shoots of alfalfa can be concluded that the distribution of secondary metabolites in plants, the same as the primary metabolites, is mainly dependent on the plant organ and tissue. Furthermore, the maximum content of these metabolites in the vegetative stage of alfalfa is due to the transition from the vegetative stage to the reproductive stage in these plants. Therefore, the late vegetative stage is the best phenological stage and the most suitable harvest time for medicinal applications.

Isolation and characterization of phenol degrading bacteria from Persian Gulf

Negar Amini Boroujeni; Mehdi Hassanshahian; Sayed Mohammad Reza Khoshrou

Volume 2, Issue 2 , February 2014, , Pages 408-416

Abstract

Phenol and phenol compounds are environmental pollutants present in industrial wastewaters such as, coal tar, oil refineries and petrochemical plants exist. Phenol removal from industrial ... Read More

International Journal of Advanced Biological and Biomedical Research

Articles in Press

Current Issue

Volume 12 (2024)

Volume 11 (2023)

Volume 10 (2022)

Volume 9 (2021)

Volume 8 (2020)

Volume 7 (2019)

Volume 6 (2018)

Volume 5 (2017)

Volume 4 (2016)

Volume 3 (2015)

Volume 2 (2014)

Volume 1 (2013)

Keywords = Phenol

The Correlation of Some Secondary Metabolites of Alfalfa (Medicago sativa L.) with Plant Organ and Harvest Time

Abstract

Isolation and characterization of phenol degrading bacteria from Persian Gulf

Abstract