Document Type: Original Article


1 MSc. student, department of fisheries, faculty of Fishery and environmental, Gorgan University of agricultural sciences and natural resources, Gorgan, Iran

2 Associate professor, department of fisheries, faculty of Fishery and environmental, Gorgan University of agricultural sciences and natural resources, Gorgan, Iran


Objective: In this survey, we have checked the effects of natural (red bell pepper and tomato) and synthetic (astaxanthin and β-carotene) pigments on bloods factors of Flower horn (Cichlasoma sp.). Methods: Five treatments (three replicates/treatment) were employed. Fishes were separated in fifteen equal groups and were fed for eight weeks with diets containing10 g kg–1 red bell pepper and tomato and 100 mg kg-1 astaxanthin and β-carotene. At the end of trial blood samples were taken from 5 fish that caught randomize by cutting the caudal stem. Results: Results of blood factors showed there is significant differences between WBCs, RBCs, Hb, HCT and Lymphocytes between treatments (p<0.05). The best result observed in treatment fed with red bell pepper. Between The factors of Monocytes and Neutrophils, there was no significant difference between treatments (p>0.05). The results showed that the use of red bell pepper in diets for Flower horn is effective and profitable in its ration.


Main Subjects

Amaninejad P, Emadi H, Emtiazjoo M,
Hosseinzadehsahafi, H (2009) Effects of Dunaliella

microalgae (Dunaliella salina) on different immune indices (complements c, and antioxidant capacity) in rainbow trout (Oncrohynchusmykiss). Scientific and Research Journal of Marine Biology. Islamic Azad University, Ahvaz. 6:3-21.
Amar E.C, Kiron V, Satoh S, Watanabe T (2003) Enhancement of innate immunity in rainbow trout (Oncorhynchus mykiss, Walbaum) associated with dietary intake of carotenoids from natural products. Fish and
Shellfish Immunology. 16:527-537.
Anderson D.P (1992) Immune stimulants, adjuvants, and vaccine carriers in fish: application to aquaculture. Annual Review of Fish Diseases. 2:281-307
Barton B.A (2002) Stress in fishes: A diversity of responses with particular reference to changes in circulating corticosteroids. Integrative and Comparative Biology. 42:517-525.
Bricaud A, Morel A, Babin M, Allali K, Claustre H (1998) Variations of light absorption by suspended particles with Chlorophyll a concentration in oceanic (case 1) waters: analysis and implications for bio optical models. Journal Geophysical Research. 103(13):31033-31044.
Blazer V.S (1992) Nutrition and disease resistance in fish. Annual Review of Fish Diseases. 2: 309-323.
Bjerkeng B, Storebakken T, Liaaen-Jensen S (2000) Response to carotenoids by rainbow trout in the sea: resporption and metabolism of dictary astaxanthin and canthaxanthin. Aquaculture. 91:153-162.
Chatzifotis S, Pavlidis M, Jimeno CD, Vardanis G, Sterioti A, Divanach P (2005) The effect of different carotenoid sources on skin coloration of cultured red porgy (Pagrus pagrus). Aquaculture Research. 36:1517-1525.
Christiansen R, Torrissen O.J (1996) Growth and survival of Atlantic salmon (Salmo salar L.) fed different dietary levels of astaxanthin. Aquaculture Nutrition. 2:55-62.
Christiansen R, Torrissen O.J (1997) Effects of dietary astaxanthin supplementation on fertilization and egg survival in Atlantic salmon (Salmo salar L.). Aquaculture. 153:51-62.
Christiansen R, Glette J, Lie O, Torrissen O.J, Waagbo R (1995) Antioxidant status and immunity in Atlantic salmon (Salmo salar L.), fed semi-purified diets with and without astaxanthin supplementation. Journal of Fish
Diseases. 18:317-328.
Christiansen R, Lie O, Torrissen O.J (1995) Growth and survival of Atlantic salmon (Salmo salar L.), fed different dietary levels of astaxanthin: first-feeding fry. Aquaculture Nutrition. 1:189-198.
Christiansen R, Lie O, Torrissen O.J (1994) Effect of astaxanthin and vitamin A on growth and survival during first feeding of Atlantic salmon (Salmo salar L.)
Aquaculture Fish Manager. 25:903-914.
Craik J.C.A (1985) Egg quality and egg pigment content in salmonid fishes. Aquaculture. 47:6168.
Goodwin T.W (1980) The biochemistry of carotenoids, 2 nd ed., Chapman and Hall, London. 291 pp.
Gouveia L, Gomes E, Empis L (1997) Use of Chlorella vulgarisin diets for rainbow trout (Oncorhynchus mykiss) to enhance pigmentation of muscle. Journal Applied Aquaculture-Culture. 7:61-70.
Ghiasvand Z, Shapouri M (2006) The Effect of Natural and synthetic Pigments on the colour of the Albino Oscar (Astronotus ocellatus sp., Agassiz, 1831). Journal of Sea Biology. 2:75-83.
Gross J (1991) Pigments in vegetables. New York. Van Nostrand Reinhold. 8: 99-102.
Guerin M, Huntley M.E, Olsubjectaizola M (2003) Haematococcus astaxanthin: applications for human health and nutrition. Trends in Biotechnology. 21: 210-
Haley P.J, Wieser M.G (1985) Erytrocytevolum distribution in rainbow trout. Veterinary Research. 46(10):2210-2212.
Jamalzade H, Keyvan A, Jamili S.H, Oryan S.H, Saeedi A (2001) Study of some blood indices on Salmo trutta Caspius. Journal of Fisheries. 1:25-26.
Knoblich M, Anderson B, Latshaw D (2005) Analyses of tomato peel and seed byproduct and their use as a source of carotenoids. Journal of the Science of Food and Agriculture. 85:1166-1170.
Pickering A.D, Pottinger T.G, Christie P (1982) Recovery of the brown trout (Salmo trutta L.), from acute handling stress: a time-course study. Journal of Fish Biolog. 20:229-244.
Landolt M (1989) The relationship between diet and the immune response of fish. Aquaculture. 79:193-206.

Lall S.P, Olivier G (1993) Role of micronutrients in immune response and disease resistance in fish. In: INRA Ed., Fish Nutrition in Practice Les collogues. 61:101-118.
Lorenz R.T, Cysewski G.R (2000) Commercial potential for Haematococcus microalgae as a natural source of astaxanthin. Trends Biotechnology. 18:160-167.
MacCarthy D.H, Stevenson J.P, Roberts M.S (1973) Some blood parameters of Rainbow trout (Salmo gairdneri). Journal of Fish Biology. 5:1-8.
Maule A.G, Tripp R.A, Kaattari S.L, Schreck C.B (1989) Stress alters the immune function and disease resistance in Chinook salmon (Oncorhynchus tshawytscha). Journal of Endocrinology. 120:135-142.
McMahan C.D, Geheber A.D, Piller K.R (2010) Molecular systematics of the enigmatic Middle American genus Vieja (Teleostei: Cichlidae). Molecular Phylogenetics and Evolution. 57:1293-1300.
Mobli M, Piraste B (1994) Vegetable production. Isfahan University of Technology. 877 pp.
Nakano T, Kanmuri, T, Takeuchi M (1995) Improvement of biochemical features in fish health by red yeast and synthetic astaxanthin. Journal of Agriculture and Food Chemistry. 43:1570-1573.
Nico LG, Beamish W.H, Musikasinthorn P (2007) Discovery of the invasive Mayan Cichlid fish (Cichlasoma urophthalmusn) in Thailand, with comments on other introductions and potential impacts. Aquatic Invasions.
Ng H, Tan H.H (2010) An annotated checklist of the nonnative freshwater fish species in the reservoirs of Singapore. Cosmos. 6:95-116.
Ong A.SH, Tee ES (1992) Natural sources of carotenoids from plants and oils. Meth Enzymology. 213:142-167.
Raymundo A, Gouveida L, Batista A.P, Empis J, Sousa I (2005) Fat mimetic capacity of Chlorella vulgaris biomass in oil in water food emulsions stabilized by pea protein. Food Research Institute. 38:961-965. 
Rehulka J (2000) Influence of astaxanthin on growth rate, condition and some blood indices of Rainbow Trout (Oncorhynchus mykiss). Aquaculture Elsevier. 190:27-47.
Sanchez-Moreno C, Plaza L, Ancos B, Cano M.P (2006) Nutritional characterization of commercial traditional pasteurized tomato juices: carotenoids, vitamin C and radical-scavenging capacity. Food Chemical. 98:749-756.
Segner H, Aren P, Von-Poeppinghaussen K, Schmidt H (1989) The effect of feeding astaxanthin to Oreochromis niloticus and Colisa labiosa on the histology of the liver. Aquaculture. 79:381-390.
Talebi M, Khara H, Zoriehzahra J, Ghobadi Sh, Khodabandelo A, Mirrasooli E (2013) Study on effect of red bell pepper on growth, pigmentation and blood
factors of rainbow trout (Oncorhynchus mykiss). World Journal of Zoology. 8 (1):17-23.
Torrissen O.J (1984) Pigmentation of salmonids-effect of carotenoids in eggs and start-feeding diet on survival and growth rate. Aquaculture. 43:185-193.
Torrisen O.J, Hardy R.W, Shearer K.D (1989) Pigmentation of salmonids-carotenoid depositions and metabolism. Critical Reviews in Environmental Science and Technology. 1(2):209-225.
Toor R.K, Savage G.P (2005) Antioxidant activity in different fractions of tomatoes. Food Research Institute. 35:487-494.
Tsatsaronis G.C, Boskou D.G (1975) Aminoacid and mineral salt content of tomato seed and skin waste. Journal of the Science of Food and Agriculture. 29:421-423.
Torrisen O.J, Hardy R.W, Shearer, K.D (1989) Pigmentation of salmonids-carotenoid deposition and metabolism. Critical Reviews in Environmental Science and Technology. 1:209-225.
Torrissen O.J, Hardy R.W, Shearer K.D, Scott T.M, Stone F.E (1996) Effect of dietary lipid on apparent digestibility coefficients for canthaxanthin in Rainbow trout (Oncorhynchus mykiss). Aquaculture. 88:351-362.
Wedemeyer G.A (1996) Physiology of fish in intensive culture systems. Chapman & Hall, New York. 232pp. Wozniake M (1996) The role of carotenoids in fish. Protectio Aquarum ET Piscatoria. Acta Societatis
Botanicorum Poloniae. 22:65-75.